A Productive Expression Platform Derived from Host-Restricted Eilat Virus: Its Extensive Validation and Novel Strategy

Most alphaviruses are transmitted by mosquitoes and infect a wide range of insects and vertebrates. However, Eilat virus (EILV) is defective for infecting vertebrate cells at multiple levels of the viral life cycle. This host-restriction property renders EILV an attractive expression platform since it is not infectious for vertebrates and therefore provides a highly advantageous safety profile. Here, we investigated the feasibility of versatile EILV-based expression vectors. By replacing the structural genes of EILV with those of other alphaviruses, we generated seven different chimeras. These chimeras were readily rescued in the original mosquito cells and were able to reach high titers, suggesting that EILV is capable of packaging the structural proteins of different lineages. We also explored the ability of EILV to express authentic antigens via double subgenomic (SG) RNA vectors. Four foreign genetic materials of varied length were introduced into the EILV genome, and the expressed heterologous genetic materials were readily detected in the infected cells. By inserting an additional SG promoter into the chimera genome containing the structural genes of Chikungunya virus (CHIKV), we developed a bivalent vaccine candidate against CHIKV and Zika virus. These data demonstrate the outstanding compatibility of the EILV genome. The produced recombinants can be applied to vaccine and diagnostic tool development, but more investigations are required.

Estimador de canal basado en sensado compresivo y LDPC para OFDM usando SDR

Este trabajo propone la aplicación de un estimador de canal basado en sensado compresivo (CS, del inglés Compressive Sensing) sobre un sistema que usa multiplexación por división de frecuencias ortogonales (OFDM, del inglés Orthogonal Frequency Division Multiplexing) usando dispositivos de radio definido por \emph{software} (SDR, del inglés Software Defined Radio). La aplicación de la teoría de CS se da a través del uso de algoritmos de reconstrucción dispersa como Orthogonal Matching Pursuit (OMP) y Compressive Sampling Matching Pursuit (CoSaMP) con el fin de aprovechar la naturaleza dispersa de las subportadoras piloto usadas en OFDM optimizando el ancho de banda del sistema. Además, para mejorar el rendimiento de estos algoritmos, se utiliza el concepto de la matriz de comprobación de paridad dispersa que se implementa en el despliegue de códigos de comprobación de paridad de baja densidad (LDPC, del inglés Low Density Parity Check) para obtener una matriz de sensado que mejore la propiedad de restricción isométrica (RIP, del inglés Isometric Restriction Property) perteneciente al paradigma de CS. El documento muestra el modelo implementado en los equipos SDR analizando la tasa de error de bit y la cantidad de símbolos piloto usados.

Sets of p-restriction and p-spectral synthesis

In this paper we investigate the restriction problem. More precisely, we give sufficient conditions for the failure of a set E in ℝn to have the p-restriction property. We also extend the concept of spectral synthesis to Lp(ℝn) for sets of p-restriction when p > 1. We use our results to show that there are p-values for which the unit sphere is a set of p-spectral synthesis in ℝn when n ⩾ 3.