Synthesis, Structural Studies, and Anticancer Properties of [CuBr(PPh3)2(4,6-Dimethyl-2-Thiopyrimidine-κS]

CuBr(PPh3)2(4,6-dimethylpyrimidine-2-thione) (Cu-L) was synthesized by stirring CuBr(PPh3)3 and 4,6-dimethylpyrimidine-2-thione in dichloromethane. The crystal structure of Cu-L was obtained, and indicated that the complex adopts a distorted tetrahedral structure with several intramolecular hydrogen bonds. Moreover, a centrosymmetric dimer is formed by the intermolecular hydrogen bonding of the bromine acceptor created by symmetry operation 1−x, 1−y, 1−z to the methyl group (D3 = C42) of the pyrimidine–thione ligand. HSA-binding of Cu-L and its ligand were evaluated, revealing that Cu-L binds to HSA differently than its ligand. The HSA-bindings were modeled by molecular docking, which suggested that Cu-L binds to the II A domain while L binds between the I B and II A domains. Anticancer activities toward OVCAR-3 and HeLa cell lines were tested and indicated the significance of the copper center in enhancing the cytotoxic effect; negligible toxicities for L and Cu-L were observed towards a non-cancer cell line. The current study highlights the potential of copper(I)-phosphine complexes containing thione ligands as therapeutic agents.

Origins of Internal Regioselectivity in Copper-Catalyzed Borylation of Terminal Alkynes

<p>Installation of a boron functionality into a more substituted carbon of terminal alkynes has been a challenging issue in chemical synthesis, since inherently Lewis acidic boron moieties, in principle, favor their attachment to a terminal carbon. Herein, we report on the highly internal-selective borylation of terminal alkynes under copper catalysis, wherein diminishment of boron-Lewis acidity and ligand-derived steric bulk around a copper center are the key to the success. In particular, the use of an anthranilamide-substituted boron moiety [B(aam)] is of high synthetic significance, because its properly diminished Lewis acidity enabled the internal regioselectivity and the Suzuki–Miyaura cross-coupling activity to be compatibly achieved. This method provided direct and universal approach to variously substituted branched alkenylboron compounds, regardless of electronic and steric properties of a substituent on terminal alkynes.</p>

Origins of Internal Regioselectivity in Copper-Catalyzed Borylation of Terminal Alkynes

<p>Installation of a boron functionality into a more substituted carbon of terminal alkynes has been a challenging issue in chemical synthesis, since inherently Lewis acidic boron moieties, in principle, favor their attachment to a terminal carbon. Herein, we report on the highly internal-selective borylation of terminal alkynes under copper catalysis, wherein diminishment of boron-Lewis acidity and ligand-derived steric bulk around a copper center are the key to the success. In particular, the use of an anthranilamide-substituted boron moiety [B(aam)] is of high synthetic significance, because its properly diminished Lewis acidity enabled the internal regioselectivity and the Suzuki–Miyaura cross-coupling activity to be compatibly achieved. This method provided direct and universal approach to variously substituted branched alkenylboron compounds, regardless of electronic and steric properties of a substituent on terminal alkynes.</p>

Reactions of a pincer proligand with copper iodide: bridging instead of C–H metalation

Various precursors of divalent copper have been treated with the meta-disubstituted phenylene-based proligand POC(H)OP (1,3-(i-Pr2PO)2C6H4) with the objective of preparing classical pincer complexes (POCOP)CuX. However, in no case was such species obtained, presumably owing to the difficult C–H metallation step. Analogous reactions of monovalent precursors were also unsuccessful, whereas reaction of POC(H)OP with CuI under different conditions gave the non-metallated adducts {(μ, κP, [Formula: see text]-POC(H)OP)Cu(μ-Ι)}2, 1, {(μ, κP, [Formula: see text]-POC(H)OP)Cu2(μ-Ι)2(DMAP)2}, 2 (DMAP = 4-dimethylaminopyridine), and {(μ, κP, [Formula: see text]-POC(H)OP)Cu2(μ3-Ι)2}2, 3. Treating 1 with DMAP gave the adduct 2, whereas 3 could be obtained by treating 1 with BuLi or by sublimation of 1. The solid state structures of these complexes revealed the tetrahedral geometry that might be anticipated for the d10 Cu(I) centers, in addition to fairly close I–H distances; on the other hand, no C–H interaction (agostic or otherwise) was observed with the Cu centers in any of these structures. The unsuccessful metallation of the C(2)–H moiety is thought to be a result of the strong preference of monovalent copper center to form bridging interactions with iodide and the POC(H)OP ligand; this appears to prevent the approach of the central carbon of the ligand to the Cu centers.

Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase

Biocatalytic copper centers are generally involved in the activation and reduction of dioxygen, with only few exceptions known. Here we report the discovery and characterization of a previously undescribed copper center that forms the active site of a copper-containing enzyme thiocyanate dehydrogenase (suggested EC 1.8.2.7) that was purified from the haloalkaliphilic sulfur-oxidizing bacterium of the genus Thioalkalivibrio ubiquitous in saline alkaline soda lakes. The copper cluster is formed by three copper ions located at the corners of a near-isosceles triangle and facilitates a direct thiocyanate conversion into cyanate, elemental sulfur, and two reducing equivalents without involvement of molecular oxygen. A molecular mechanism of catalysis is suggested based on high-resolution three-dimensional structures, electron paramagnetic resonance (EPR) spectroscopy, quantum mechanics/molecular mechanics (QM/MM) simulations, kinetic studies, and the results of site-directed mutagenesis.

Highly enantioselective carbene insertion into N–H bonds of aliphatic amines

Aliphatic amines strongly coordinate, and therefore easily inhibit, the activity of transition-metal catalysts, posing a marked challenge to nitrogen-hydrogen (N–H) insertion reactions. Here, we report highly enantioselective carbene insertion into N–H bonds of aliphatic amines using two catalysts in tandem: an achiral copper complex and chiral amino-thiourea. Coordination by a homoscorpionate ligand protects the copper center that activates the carbene precursor. The chiral amino-thiourea catalyst then promotes enantioselective proton transfer to generate the stereocenter of the insertion product. This reaction couples a wide variety of diazo esters and amines to produce chiral α-alkyl α–amino acid derivatives.