Dielectrophoresis (DEP) is a label-free, accurate, fast, and low-cost diagnostic technique that uses the principles of polarization and the motion of bioparticles in applied electric fields. DEP occurs when uncharged particles in the solution are subject to a spatially non-uniform alternating-current (AC) electric field, resulting in the motion of particles by creating a polarizability gradient between the particles and the suspending medium. The movement of particles in DEP is based on the difference in polarizability between the particles and the surrounding medium. If the particles move toward the electrode edge, the region of high electric field gradient, the response is called positive DEP (p-DEP). At the same time, if the particles move away from the electrode edge, the response is called negative DEP (n-DEP). This phenomenon provides a powerful and versatile tool for the non-destructive manipulation of nanoscale materials, allowing for the control of the resistance and the type of the assembly. This technique has been proven to be beneficial in various fields, including environmental research, polymer research, sensors, biosensors, microfluidics, medicine, and diagnostics. This paper reviews the fundamentals of DEP and its specific application in the incorporation of graphene, graphene oxide(GO), and reduced graphene oxide(RGO), enabling the assembly of individual two-dimensional nanostructures at predefined locations in microdevices for gas sensor applications. The review provides an essential framework for parallel fabrication approaches of graphene-based devices.