Highly sensitive in vitro bioassay for Luteinizing Hormone and Chorionic Gonadotropin

IIn previous studies, we had shown the synergistic effect of 10-5 M forskolin (FSK) on the detection threshold of the cyclic AMP response to Luteinizing Hormones (LH) and Chorionic Gonadotropins (CG) from various species in the mouse Leydig Tumor cell (mLTC) cell line. Indepedently, we had started to study the effect of 10-12 – 10-6 M OXT also on the cyclic AMP response to LH and CG preparations on these same cells and found an amplifying effect on the luminescence response caused by gonadotropins. The aim was then to explore the effects of 10-12 – 10-6 M OXT on the gonadotropin-induced cAMP response, in the presence or absence of 10 µM FSK to optimize the assay down to a sensitivity compatible with the detection of the circulating concentrations of these hormones in various species. Finally the optimization relies on three independent phenomena: 1/ the inhibition of nucleotide phosphodiesterase by IBMX to avoid cAMP degradation, 2/ the strong synergy of 10 µM forskolin with low concentrations of LH or CG during the 1-hour luminescence measurement and, 3/ the stimulatory effect of 10-8M oxytocin on the amplitude of transfected cAMP-sensitive luciferase response. By doing this, the detectable concentrations are at the 1-10 pg/well (pM range) for the LHs and CGs from various species. The bioactivities of circulating LHs and CGs in blood or urine are therefore expected to be measurable in 10µL-plasma samples from mammalian species and maybe others.

A ultrastable cucurbit[6]uril-based multifunctional supramolecular assembly for efficient detection of nitroaromatic compounds and antibiotics

A CB[6]-based supramolecular assembly: [Zn(QDS)2]·(CB[6])2 (1) (CB[6] = cucurbit[6]uril, HQDS = 8-Hydroxy-5-quinolinesulfonic acid hydrate) has been synthesized under solvothermal conditions. Luminescence measurement reveals that 1 can serve as a fast...

New Avenues in Radical Trifluoromethylselenylation with ­Trifluoromethyl Tolueneselenosulfonate

We demonstrated that the shelf-stable reagent trifluoromethyl tolueneselenosulfonate can be involved in radical trifluoromethylselenylation. Upon visible-light irradiation, the homolysis of the reagent could take place at room temperature. This finding is explored for unprecedented C(sp2)–SeCF3 and C(sp3)–SeCF3 processes under transition-metal-free conditions. Mechanistic investigations, including cyclic voltammetry, luminescence measurement, and EPR studies, allowed the proposal of plausible mechanisms.1 Introduction.2 Reactivity of Reagent I with Diazonium Salts3 Reactivity of Reagent I with Alkenes and Alkynes4 Conclusion

3D Instantaneous Reconstruction of Turbulent Industrial Flames Using Computed Tomography of Chemiluminescence (CTC)

Computed Tomography of Chemiluminescence (CTC) was used to reconstruct the instantaneous three-dimensional (3D) chemiluminescence field of a high-power industrial flame, which was made optically accessible, for the first time. The reconstruction used 24 projections that were measured simultaneously, in one plane and equiangularly spaced within a total fan angle of 172.5°. The 3D results were examined by plotting both vertical and horizontal slices, revealing highly wrinkled structures with good clarity. The results presented are one of a series of experimental demonstrations of CTC applications to turbulent gaseous flames. The work reveals the potential to use any kind of luminescence measurement, such as emission from heated particles in coal-fired flames, for analysis of the flame shape directly in 3D.