Siderophores are produced by microorganisms in iron-deficient environments. They are classified by structure as hydroxamate, catecholate, carboxylate or mixed type siderophores. These differences are also reflected in the selectivity for other valuable elements than iron, which allows designating them as “metallophores”, and makes them of interest for several industrial and medical applications. Thus, it is essential to understand the biosynthesis of these molecules to increase the set of available metallophores that are stable and suited for the respective applications. The probable structure of the metallophore from T. agreste DSM 44070 was predicted by similarity search and gene annotation. An N-hydroxylating monooxygenase (NMO: TheA) of T. agreste DSM 44070 that catalyzes an initial step was synthesized and characterized in detail. The respective metallophore was synthesized, purified and studied. The structure prediction suggested a hydroxamate-type (Erythrochelin-like) metallophore that contains L-N5-hydroxyornithine. This precursor is synthesized by TheA. The siderophore designated as “Thermochelin” is produced, extracted and purified successfully. Complexation was confirmed by CAS-assay. In this study, we expanded the scope of siderophores and the knowledge towards their biosynthetic pathways. Thermochelin is the second siderophore, which was purified from a thermophilic organism, and TheA is the first NMO, which was characterized from an extremophile.