Abstract
The objective of this study was to find a biopolymer producing potential actinobacteria candidate from polluted soil. A biopolymer fabricating Streptomyces toxytricini D2 was isolated and characterized by 16S rRNA sequencing. The S. toxytricini D2 produced polymeric granules were categorized by FTIR, 1H NMR, and 13C NMR analyses and confirmed that the produced granules were Polyhydroxyalkanoates (PHAs). The S. toxytricini D2 has the competence to fabricate 86.56% of PHAs (23.64 g L-1 of PHAs from 27.31 g L-1 of biomass) under the optimized growth conditions: 8% of tapioca molasses, 4% of (NH4)2SO4, 8% of inoculum, pH 6.5, 30 ºC and 72 h of incubation. These results concluded that this is the novel report about the S. toxytricini D2 as could be used as an efficient candidate for the mass production of PHAs polymer under optimized conditions with low-cost carbon source and in a short duration of the production process.