Phyllantus Emblica Mediated Silica Nanomaterials: Biosynthesis, Structural and Stability Analysis

The current investigation reports on a green route, simple and eco-friendly method for synthesis of silica nanoparticles from Phyllantus emblica. Appropriate characterization techniques were employed to assess the crystalline nature, microstructure, size, purity, elemental composition and stability of as-biosynthesized silica nanoparticles. The XRD analysis showed a wide-ranging peak at 22∘ of 2θ value and proved that the nanoparticles were crystalline nature with 32 nm average size of particles. FT-IR studies confirmed the occurrence of metal oxide group and presence of phyto-molecules namely hydroxyl, amide, and carboxyl functional groups, which were responsible for formation and stabilization of silica nanomaterials. TGA and Zeta potential analysis determined that silica nanoparticles are highly thermostable. EDX analysis revealed the purity of nanomaterials and spectra confirmed that formation of silica nanomaterials (72.97 weight percentage of SiO2 content) with low impurities. SEM analysis shows that the particles are spherical in shape with low agglomeration. This research work concluded that the P. emblica was an excellent and reliable green resource for production of highly stable and potential silica nanoparticles.

Nanomaterials and Aging

As the proportion of the elderly population increases, more and more people suffer from aging-related diseases. Even if aging is inevitable, prolonging the time of healthy aging, delaying the progression of aging-related diseases, and the incidence of morbidity can greatly alleviate the pressure on individuals and society. Current research and exploration in the field of materials related to aging are expanding tremendously. Here, we present a summary of recent research in the field of nanomaterials relevant to aging. Some nanomaterials, such as silica nanomaterials (NMs) and carbon nanotubes, cause damage to the cells similar to aging processes. Other nanomaterials such as fullerenes and metalbased nanomaterials can protect the body from endogenous and exogenous harmful substances such as ROS by virtue of their excellent reducing properties. Another new type of nucleic acid nanomaterial, tetrahedral framework nucleic acids, works effectively against cell damage. This material selectively clears existing senescent cells in the tissue and thus prevents the development of the chronic inflammatory environment caused by senescent cells secreting senescence-associated secretory phenotype to the surroundings. We believe that nanomaterials have tremendous potential to advance the understanding and treatment of aging-related disorders, and today's research only represents the beginning stages.

Effect of Silica Based Nanoparticles against Plasmodium falciparum and Leishmania infantum parasites

Malaria and Leishmaniasis are two major parasitic diseases, endemic in large areas of tropical countries with high morbidity and mortality across the world. Nanoparticles in small sizes are specifically considered in medicine due to their ability to enter the cells, control the distribution of the administered drug and carry the drug specifically to the place of action. The present study aims to introduce the application of silica nanoparticles as new promising nanotools in malaria and leishmaniasis treatment. Ion doped silica nanomaterials revealed antileishmanial activities indicating the positive role of calcium, magnesium and copper to the surface of the particles against Leishmania parasites. Artemisinin-loaded nanoparticles presented the most promising antiparasitic properties with a sustained release able to overcome the parasite invasion. The sustainable release of artemisinin guarantee both the maintenance of its potential efficacy and also introduce an administration of drug to avoid subsequent drug resistance.

Wettability Changes Due to Nanomaterials and Alkali—A Proposed Formulation for EOR

We investigated the usage of two silica nanomaterials (surface-modified) and alkali in enhanced oil recovery through Amott spontaneous imbibition tests, interfacial tension (IFT) measurements, and phase behavior. We evaluated the wettability alteration induced by the synergy between nanomaterials and alkali. Moreover, numerical analysis of the results was carried out using inverse Bond number and capillary diffusion coefficient. Evaluations included the use of Berea and Keuper outcrop material, crude oil with different total acid numbers (TAN), and Na2CO3 as alkaline agent. Data showed that nanomaterials can reduce the IFT, with surface charge playing an important role in this process. In synergy with alkali, the use of nanomaterials led to low-stable IFT values. This effect was also seen in the phase behavior tests, where brine/oil systems with lower IFT exhibited better emulsification. Nanomaterials’ contribution to the phase behavior was mainly the stabilization of the emulsion middle phase. The influence of TAN number on the IFT and phase behavior was prominent especially when combined with alkali. Amott spontaneous imbibition resulted in additional oil recovery ranging from 4% to 50% above the baseline, which was confirmed by inverse Bond number analysis. High recoveries were achieved using alkali and nanomaterials; these values were attributed to wettability alteration that accelerated the imbibition kinetics as seen in capillary diffusion coefficient analysis.

Skin Sensitization Potential and Cellular ROS-Induced Cytotoxicity of Silica Nanoparticles

Nowadays, various industries using nanomaterials are growing rapidly, and in particular, as the commercialization and use of nanomaterials increase in the cosmetic field, the possibility of exposure of nanomaterials to the skin of product producers and consumers is increasing. Due to the unique properties of nanomaterials with a very small size, they can act as hapten and induce immune responses and skin sensitization, so accurate identification of toxicity is required. Therefore, we selected silica nanomaterials used in various fields such as cosmetics and biomaterials and evaluated the skin sensitization potential step-by-step according to in-vitro and in-vivo alternative test methods. KeratinoSensTM cells of modified keratinocyte and THP-1 cells mimicking dendritic-cells were treated with silica nanoparticles, and their potential for skin sensitization and cytotoxicity were evaluated, respectively. We also confirmed the sensitizing ability of silica nanoparticles in the auricle-lymph nodes of BALB/C mice by in-vivo analysis. As a result, silica nanoparticles showed high protein binding and reactive oxygen species (ROS) mediated cytotoxicity, but no significant observation of skin sensitization indicators was observed. Although more studies are needed to elucidate the mechanism of skin sensitization by nanomaterials, the results of this study showed that silica nanoparticles did not induce skin sensitization.

Size and surface modification of silica nanoparticles affect the severity of lung toxicity by modulating endosomal ROS generation in macrophages

Background As the application of silica nanomaterials continues to expand, increasing chances of its exposure to the human body and potential harm are anticipated. Although the toxicity of silica nanomaterials is assumed to be affected by their physio-chemical properties, including size and surface functionalization, its molecular mechanisms remain unclear. We hypothesized that analysis of intracellular localization of the particles and subsequent intracellular signaling could reveal a novel determinant of inflammatory response against silica particles with different physico-chemical properties. Results We employed a murine intratracheal instillation model of amorphous silica nanoparticles (NPs) exposure to compare their in vivo toxicities in the respiratory system. Pristine silica-NPs of 50 nm diameters (50 nm-plain) induced airway-centered lung injury with marked neutrophilic infiltration. By contrast, instillation of pristine silica particles of a larger diameter (3 μm; 3 μm-plain) significantly reduced the severity of lung injury and neutrophilic infiltration, possibly through attenuated induction of neutrophil chemotactic chemokines including MIP2. Ex vivo analysis of alveolar macrophages as well as in vitro assessment using RAW264.7 cells revealed a remarkably lower cellular uptake of 3 μm-plain particles compared with 50 nm-plain, which is assumed to be the underlying mechanism of attenuated immune response. The severity of lung injury and neutrophilic infiltration was also significantly reduced after intratracheal instillation of silica NPs with an amine surface modification (50 nm-NH2) when compared with 50 nm-plain. Despite unchanged efficacy in cellular uptake, treatment with 50 nm-NH2 induced a significantly attenuated immune response in RAW264.7 cells. Assessment of intracellular redox signaling revealed increased reactive oxygen species (ROS) in endosomal compartments of RAW264.7 cells treated with 50 nm-plain when compared with vehicle-treated control. In contrast, augmentation of endosomal ROS signals in cells treated with 50 nm-NH2 was significantly lower. Moreover, selective inhibition of NADPH oxidase 2 (NOX2) was sufficient to inhibit endosomal ROS bursts and induction of chemokine expressions in cells treated with silica NPs, suggesting the central role of endosomal ROS generated by NOX2 in the regulation of the inflammatory response in macrophages that endocytosed silica NPs. Conclusions Our murine model suggested that the pulmonary toxicity of silica NPs depended on their physico-chemical properties through distinct mechanisms. Cellular uptake of larger particles by macrophages decreased, while surface amine modification modulated endosomal ROS signaling via NOX2, both of which are assumed to be involved in mitigating immune response in macrophages and resulting lung injury.