The bioactive components of lemongrass powder have been evaluated using GC-MS. The GC-MS analysis was performed on GC-MS comprising an automatic liquid sampler and agilent gas chromatograph interfaced to mass spectrometer (GC-MS). Interpretation of the mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST). The compound bioactivity prediction is based on Dr. Duke’s phytochemical and ethnobotanical Database. GC/MS analysis of methanolic extract of lemongrass leaves revealed the existence of Pentane, 2,4-Dimethyl, Dodecanoic acid tert-butyl ester, 2,6 Bis (1,1-dimethylethyl)-4-[(4-chloro-6-(3,5, bis (1,1-dimethylethyl)-4- hydroxyanilino)-1,3,5-triazin-2-yl)amino]phenol and 3-Formyl-4,5-dimethyl-pyrrole. The presence of these compounds in the plant extract may at least be responsible for the pharmacological properties of Cymbopogon citratus and thus recommended as plant of phytopharmaceutical importance.