scholarly journals GC-MS Analysis of Bioactive Compounds in Lemongrass (Cymbopogon citratus) Powder

2021 ◽  
Vol 10 (10) ◽  
pp. 283-290
Author(s):  
Pradeep P. Thorat Nikhil D. Solanke ◽  
Jayashri D. Ughade
Keyword(s):  
Methanolic Extract ◽  
Butyl Ester ◽  
Dodecanoic Acid ◽  
Pharmacological Properties ◽  
Bioactive Components ◽  
Gas Chromatograph ◽  
Cymbopogon Citratus ◽  
Bioactivity Prediction ◽  
Ms Analysis ◽  
Amino Phenol

The bioactive components of lemongrass powder have been evaluated using GC-MS. The GC-MS analysis was performed on GC-MS comprising an automatic liquid sampler and agilent gas chromatograph interfaced to mass spectrometer (GC-MS). Interpretation of the mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST). The compound bioactivity prediction is based on Dr. Duke’s phytochemical and ethnobotanical Database. GC/MS analysis of methanolic extract of lemongrass leaves revealed the existence of Pentane, 2,4-Dimethyl, Dodecanoic acid tert-butyl ester, 2,6 Bis (1,1-dimethylethyl)-4-[(4-chloro-6-(3,5, bis (1,1-dimethylethyl)-4- hydroxyanilino)-1,3,5-triazin-2-yl)amino]phenol and 3-Formyl-4,5-dimethyl-pyrrole. The presence of these compounds in the plant extract may at least be responsible for the pharmacological properties of Cymbopogon citratus and thus recommended as plant of phytopharmaceutical importance.

scholarly journals GC-MS, Molecular Docking and In Vitro Studies of Methanolic Extract of Leaves of Jatropha gossypiifolia as Potent Anti-alzheimer’s

2021 ◽  
Vol 68 (1) ◽  
Author(s):  
S. Babyvanitha ◽  
Jaykar B.
Keyword(s):  
Molecular Docking ◽  
Methyl Ester ◽  
Methanolic Extract ◽  
Acetylcholinesterase Inhibitor ◽  
Research Work ◽  
Acid Methyl Ester ◽  
Phytochemical Analysis ◽  
Dodecanoic Acid ◽  
Ms Analysis ◽  
Jatropha Gossypiifolia

The aim of this study is to explore anti-alzheimer’s effect of phytocompounds identified from methanolic extract leaves of Jatropha gossypiifolia using GC-MS and Molecular docking studies. Acetylcholinesterase inhibitors improved cholinergic deficit in the brain which are beneficial in the treatment of Alzheimer’s disease. In this research work we studied the AchE antagonistic effect of phytocompounds identified from Jatropha gossypiifolia using an in silico approach. This work reported the phytochemical analysis of the methanolic extract of the leaves of Jatropha gossypiifolia. Eleven compounds were revealed through GC-MS analysis and screened using GC-MS QP2010PLUS, Shimadzu, Japan. Interpretation on mass spectrum of GC-MS was done using the database of National Institute Standard and Technology (NIST). 1,1,5-Trimethyl-1, 2-dihydro naphthalene, Methyl 10,12-pentacosadiynoate, Dodecanoic acid, methyl ester, Methyl tetradecanoate, 3,7,11,15-Tetramethyl-2-hexadecen-ol, Cyclopropanepentanoic acid 2-undecyl, methyl ester-trans, N-hexadecanoic acid, 9,12,15- Octadecatrienoic acid, Heptadecanoic acid, 16-methyl methyl ester, 7,8 Epoxylanostan 11-ol, 3-acetoxy, Cis,Cis, Cis – 7,10,13 – Hexadecatrienal compounds identified from methanolic leaf extract of Jatropha gossypiifolia using GC-MS analysis. Molecular docking of Jatropha gossypiifolia on human AchE protein was determined by using AutoDock Vina software and compared with Donepezil a known reversible acetylcholinesterase inhibitor. The interactions of Human Acetylcholinesterase-ligand conformations, including hydrogen bonds and various other bonds were analyzed using BIOVIA Discovery Studio 2016. Cis, Cis, Cis – 7,10,13-Hexadecatrienal existing phytochemicals from the leaves of Jatropha gossypiifolia had the highest fitness score of -7.6 kcal/mol comparable with donepezil having -9.2 kcal/mol and hence could be a potent antialzheimer’s drugs.

GC-MS ANALYSIS OF PHYTOCHEMICALS IN THE METHANOLIC EXTRACT OF Caesalpinia coriaria (Jacq) Willd.

2016 ◽  
Vol 4 (7) ◽  
pp. 1417-1422 ◽  
Author(s):  
P.T. Srinivasan ◽  
◽  
D. Anandhi ◽  
S. Kanimozhi ◽  
M. Anbarasan. ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Ms Analysis

scholarly journals UPLC-HRESI-MS and GC-MS analysis of the leaves of Nicotiana glauca

2021 ◽  
Vol 72 (1) ◽  
pp. 97-108
Author(s):  
Rafeef K. Massadeh ◽  
Tamam El-Elimat ◽  
Mohammad Al-Gharaibeh ◽  
Khaled Tawaha ◽  
Feras Q. Alali
Keyword(s):  
High Resolution ◽  
Plant Material ◽  
Methanolic Extract ◽  
Volatile Oil ◽  
Ethyl Linoleate ◽  
Major Constituent ◽  
Hexadecanoic Acid ◽  
Nicotiana Glauca ◽  
Plant Oil ◽  
Ms Analysis

Abstract The alkaloid-rich fraction obtained by fractionation of the crude methanolic extract of the leaves of wild tobacco tree Nicotiana glauca Graham (Solanaceae) was analyzed using UPLC-MS and GC-MS. Anabasine, a piperidine alkaloid, was identified as the major constituent with approximately 60 % (m/m) of the alkaloid-rich fraction. In addition to anabasine, six secondary metabolites were identified using high-resolution UPLC-MS. Anabasine was quantified in the leaves to be 1 mg g−1 dry plant material. The GC-MS analysis revealed five compounds with anabasine as the major component, while nicotine was not detected. Moreover, GC-MS was used for the analysis of the volatile oil that was obtained by hydro-distillation from the leaves of N. glauca. The volatile plant oil was found to be rich in oxygenated sesquiterpenes (e.g., β-bisabolol) and carboxylic acids and esters (e.g., ethyl linoleate and hexadecanoic acid), whereas anabasine was not detected.

scholarly journals Potential insecticidal activity of four essential oils against the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7767-7783
Author(s):  
Mohamed E. Tawfeek ◽  
Hayssam M. Ali ◽  
Mohammad Akrami ◽  
Mohamed Z. M. Salem
Keyword(s):  
Mass Spectrometry ◽  
Alkaline Phosphatase ◽  
Sitophilus Oryzae ◽  
Imperata Cylindrica ◽  
Rice Weevil ◽  
Gas Chromatograph ◽  
Cymbopogon Citratus ◽  
Phytochemical Composition ◽  
Germacrene D ◽  
Gas Chromatograph Mass Spectrometry

Oils extracted from Cymbopogon citratus, Lantana camara, Artemisia camphorata, and Imperata cylindrica plants were used as potential insecticides against the rice weevil, Sitophilus oryzae (L.) (Coleoptera: Curculionidae). The phytochemical composition of the isolated oils was identified by gas chromatograph-mass spectrometry (GC-MS). Oil contact toxicities were evaluated against the adults of S. oryzae. The activities of acetylcholinesterase (AChE), alkaline phosphatase (ALP), and transaminases enzymes (AST) were measured. L. camara oil (LC50 = 9.81 mg/cm2) demonstrated the highest effect, followed by C. citratus oil (LC50 = 10.89 mg/cm2), A. camphorata EO (LC50 = 16.12 mg/cm2), and I. cylindrica oil (LC50= 36.85 mg/cm2) against the adults of S. oryzae. The inhibition percentages of AChE were 38.8, 41.7, 35.0, and 27.2%; ALP were 42.4, 49.3, 28.1, and 18.7%; AST were 33.9, 38.7, 20.8, and 11.8%; and ALT were 22.7, 30.5, 14.6, and 9.6% after treated S. oryzae with oils from C. citratus, L. camara, A. camphorata and I. cylindrica, respectively. The highest abundant compounds in C. citratus were geranial (25.95%), nerylacetal (8.85%), and neral (8.45%), in L. camara were caryophyllene (12.2%), and 3-elemene (8.89%), in A. camphorata were germacrene D-4-ol (20.83%), and borneol (19.47%), and in I. cylindrica were 5-phenylundecane (10.68%), and 6-phenyldodecane (8.70%).

Eugenol Found by GC-MS Analysis of The Methanolic Extract of The Fruit Pulp of Indigenous Mango (Mangifera indica L.) Variety, Champa from Murshidabad District of West Bengal, India

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Mitu De ◽  
Susanta Ray ◽  
Subhasree Dutta ◽  
Santi Ranjan Dey
Keyword(s):  
Volatile Compounds ◽  
West Bengal ◽  
Methanolic Extract ◽  
Mangifera Indica ◽  
Fruit Pulp ◽  
Ms Analysis ◽  
Bioactive Component ◽  
Herbal Plants ◽  
Michelia Champaca

Indigenous mango (Mangifera indica L.) varieties have unique aroma, taste, flavour, texture and size. The interactions among volatile compounds and with sugars and acids have a role in the distinct mango flavor. Murshidabad district of West Bengal has several indigenous mango varieties that have unique attributes like taste and aroma. The indigenous Champa mango variety of Murshidabad district has a distinct aroma very similar to that of the champaka flower (Michelia champaca L.). The present investigation was to determine if there was any phyto-chemical which could be responsible for the distinct and unique aroma present in the mango variety Champa. Eugenol, a phytogenic bioactive component frequently found in diversified herbal plants was found by GC-MS analysis of the methanolic extract of the fruit pulp of indigenous mango variety, Champa.

Py-GC-MS Analysis on Benzene-Alcohol Extractives of Eucalyptus urophydis Wood for Fine Indoor Environment

2012 ◽  
Vol 178-181 ◽  
pp. 288-291
Author(s):  
Wan Xi Peng ◽  
Lan Sheng Wang ◽  
Zhi Lin ◽  
Zhen Zhen Zheng
Keyword(s):  
Health Care ◽  
Indoor Environment ◽  
Decanoic Acid ◽  
Alcohol Solution ◽  
Dodecanoic Acid ◽  
Pyrolysis Products ◽  
Ms Analysis ◽  
Eucalyptus Wood ◽  
Biological Medicine

In order to evaluate the safety of Eucalyptus wood interior, Eucalyptus urophydis wood was used and extracted in benzene-alcohol solution, and its extractives were identified by Py-GC/MS. The main and abundant constituents of 350°С pyrolysis products were 1-phenanthrenecarboxylic acid, 1,2,3,4,4a,9,10,10a-octahydro-1,4a-dimethyl-7-(1-methylethyl)-(5.38%), stigmasterol,22,23- dihydro- (4.67%), 3'-chlorooxanilic acid n'-(3-ethox y-4-hydroxybenzylidene)hydrazide (4.13%), stigmast-5-en-3-ol, oleate(3.83%), 2-ethylacridine(3.32%), etc. The main and abundant constituents of 600°Сpyrolysis products were decanoic acid, 1,2,3-propanetriyl ester(33.8%), dodecanoic acid, 1,2,3-propanetriyl ester(8.03%), decanoic acid, 1,2,3-propanetriyl ester(3.99%), 1-chloromethyl-1-(2-propenyloxy)-1-silacyclohexane(3.98%), etc. So there was much biological medicine and health care composition in Eucalyptus urophydis wood. And the wood was safety.

scholarly journals IN VITRO CALLUS INDUCTION AND COMPARATIVE GC-MS ANALYSIS OF METHANOLIC EXTRACTS OF CALLUS AND LEAF SAMPLES OF AMPELOCISSUS LATIFOLIA (ROXB.) PLANCH

2018 ◽  
Vol 10 (9) ◽  
pp. 68
Author(s):  
Deep Chhavi Anand ◽  
Rishikesh Meena ◽  
Vidya Patni
Keyword(s):  
Callus Induction ◽  
Culture Media ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Dodecanoic Acid ◽  
Stem Explants ◽  
Ms Analysis ◽  
Wide Range

Objective: The aim of the present study was to develop a callus induction protocol and comparative study of therapeutic phytochemicals present in in vivo leaf and in vitro callus extracts through Gas Chromatography-Mass Spectrometry analysis.Methods: Murashige and Skoog media was used as culture media for callus induction. In vitro callus induction protocol was developed by studying the effects of various plant growth regulators like auxin, 2, 4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalic acetic acid), alone and in combination with cytokinin BAP (benzyl aminopurine), on leaf and stem explants. The GC-MS analysis of Ampelocissus latifolia was carried out on Shimadzu QP-2010 plus with thermal desorption system TD 20 to study the phytochemical profile.Results: In vitro callus induction protocol was developed for the plant and callusing was done from leaf and stem explants of Ampelocissus latifolia. The best result for callus induction was obtained using leaf explant, and callus production were maximum in Murashige and Skoog medium fortified with BAP (0.5 mg/l) and NAA (1.0 mg/l). Major compounds identified in the GC-MS analysis were Campesterol, Stigmasterol, Beta-Sitosterol, Docosanol, Dodecanoic acid, etc., in in vitro extract and Beta Sitosterol, Tocopherol, Squalene, Bergamot oil, Margarinic acid, Hexadecanoic acid, etc., in in vivo extract. The different active phytochemicals identified have been found to possess a wide range of biological activities, thus this analysis forms a basis for the biological characterization and importance of the compounds identified for human benefits.Conclusion: This is the first report on callus induction in Ampelocissus latifolia. From the results obtained through the in vitro callus induction and its comparative GCMS analysis with in vivo extract, it is revealed that Ampelocissus latifolia contains various bioactive compounds that are of importance for phytopharmaceutical uses. The GCMS analysis revealed that the amount of Beta-sitosterol and 5-Hydroxymethylfurfural (HMF) was very high in in vitro extract as compared to in vivo extract.

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