Molecular and Biological Characterization of Haemaphysalis Leachi (Acari: Ixodidae) in Nigeria West Africa

Ticks constitute a serious threat to the wellbeing of humans and other animals. The accurate identification of ticks is paramount in epidemiological investigations. Genetic markers have been identified and used to overcome the limitations of phenotypic identification of ticks. In this study, the cytochrome c oxidase 1 (Cox1) gene was amplified and sequenced for the identification of Haemaphysalis leachi, the putative vector of Babesia rossi in Nigeria. Amplification was successful in 34 out of 39 (87 %) ticks collected from dogs in three Nigerian states with sequence homology of 99 % to H. leachi in Genbank (GenBank: MN663156.1). Maximum Likelihood phylogenetic analysis showed significant grouping of H. leachi sequences in independent monophyletic subclade with a bootstrap value of 100 %. Genetic distance analysis of H. leachi identified in this study indicated a very low level of intraspecific diversity (0.016 %) compared to 0.150—0.190 % interspecific distance to other Haemaphysalis species. The number of eggs laid by engorged female ticks maintained in the laboratory ranged from 885 to 2190 and was proportional to the ticks’ initial weight. The mean value of other biological parameters; female engorgement weight, pre-oviposition period, oviposition period, total egg mass, egg size, efficiency rates of female ticks in converting their food reservoir to eggs and incubation period are, 147.5 mg, 7.8 days, 13.2 days, 59.5 mg, 485.5 × 348.7 µm, 41.2 % and 26.2 days, respectively. This study reports the first molecular identification of H. leachi in Nigeria.

Ixodid ticks, fleas and lice infesting dogs and cats in Hawassa, southern Ethiopia

This study investigated the prevalence, risk factors and species composition of ticks, fleas and lice infesting dogs and cats in and around Hawassa in southern Ethiopia. In total, 200 dogs and 100 cats were examined from November 2008 to April 2009. Of the dogs and cats examined, 99.5% and 91.5%, respectively, were infested with one or more species of ticks, fleas or lice. The overall prevalence was higher in dogs than in cats. A total of six different species of ectoparasites were collected and identified from dogs, but only three species from cats. Ctenocephalides felis was the predominant species amongst the animals, with a prevalence of 82.9% on dogs and 67% on cats. Other prevalent species on dogs included Ctenocephalides canis (73.8%), Heterodoxus spiniger (4%), nymphs of Amblyomma spp. (3.5%), Pulex irritans (2.5%) and Haemaphysalis leachi (0.5%). C. canis (18%) and P. irritans (6%) were also found on cats. More female than male fleas and lice were observed. The study revealed that the prevalence of fleas, ticks and lice on dogs was not significantly different between male and female animals or between young and adult dogs. However, the prevalence of these ectoparasites was significantly higher in female than in male and in adult than in young cats. The study showed that the prevalence of ectoparasites on both dogs and cats was significantly higher on animals with flea allergy dermatitis (FAD) than those without FAD, and on animals with lesions on their skin compared with those without lesions.

Effect of Water Flooding on the Oviposition Capacity of Engorged Adult Females and Hatchability of Eggs of Dog Ticks:Rhipicephalus sanguineusandHaemaphysalis leachi leachi

Effects of water flooding on the oviposition capacity of engorged adult females and hatchability of eggs ofRhipicephalus sanguineusandHaemaphysalis leachi leachiunder laboratory conditions were investigated. The durations of time of water flooding were 1, 2, 4, 6, 12, 24, 48, 72, 96, and 120 hours. Engorged females ofR. sanguineusandH. leachi leachidid not oviposit after being flooded for more than 48 and 6 hours, respectively. The preoviposition periods of both species were longer than those of their controls. The number of eggs laid were significantly lower () and higher () than their controls, respectively, forR. sanguineusandH. leachi leachiflooded for 1–4 hours. The hatchability of eggs of both species decreased as flooding time increased. The percentage of hatchability was negatively correlated with flooding time and was highly significant (; ). It is concluded thatR. sanguineustolerated simulated water flooding more thanH. leachi leachi.

Small mammals as hosts of immature ixodid ticks

Two hundred and twenty-five small mammals belonging to 16 species were examined for ticks in Free State, Mpumalanga and Limpopo Provinces, South Africa, and 18 ixodid tick species, of which two could only be identified to genus level, were recovered. Scrub hares, Lepus saxatilis, and Cape hares, Lepus capensis, harboured the largest number of tick species. In Free State Province Namaqua rock mice, Aethomys namaquensis, and four-striped grass mice, Rhabdomys pumilio, were good hosts of the immature stages of Haemaphysalis leachi and Rhipicephalus gertrudae, while in Mpumalanga and Limpopo Provinces red veld rats, Aethomys chrysophilus, Namaqua rock mice and Natal multimammate mice, Mastomys natalensis were good hosts of H. leachi and Rhipicephalus simus. Haemaphysalis leachi was the only tick recovered from animals in all three provinces.

Efficacy of orally administered powdered aloe juice (Aloe ferox) against ticks on cattle and ticks and fleas on dogs

The efficacy of orally administered powdered aloe juice (Aloe ferox) was evaluated against ticks on cattle and against ticks and fleas on dogs. Twelve calves were each infested over a 25-day period with approximately 4000 larvae of Rhipicephalus (Boophilus) decoloratus and allocated to 3 groups of 4 calves each. Three days after the last larval infestation and daily for 22 days thereafter, the calves in 1 group were fed 5 mg / kg body weight and those in another 25 mg / kg body weight of powdered aloe juice incorporated in game maintenance pellets, while the animals in the 3rd group received only pellets. Detached female ticks were collected daily and counted and the weights and the fertility of groups of 50 engorged female ticks collected from the animals were ascertained. The powdered aloe juice in the game maintenance pellets had no effect on the tick burdens of the calves or on the fertility of the ticks. Six dogs, in each of 2 groups, were treated daily for 15 consecutive days, commencing on Day -5 before the 1st tick infestation, with either 0.39 g or 0.74 g of powdered aloe juice, administered orally in gelatin capsules, while a 3rd group of 6 dogs served as untreated controls. All the dogs were challenged with Haemaphysalis leachi on Days 0 and +7, and with Ctenocephalides felis on Days+1and +8, and efficacy assessments were made 1 day after flea and 2 days after tick challenge, respectively. Treatment was not effective against ticks or fleas on the dogs.

Ixodid ticks on dogs in southern Mozambique

The objective of this study was to determine the species and distribution of ticks infesting domestic dogs in southern Mozambique. To this end 89 collections were taken from dogs and the geographic coordinates of the localities at which they were made noted. Fifty-eight of these collections were from animals in the city of Maputo and 16 from animals at rural settlements close to Maputo. A further 15 collections were made from dogs in rural environments north of Maputo. Ten ixodid tick species, of which one was only identified to genus level, were recovered. Rhipicephalus sanguineus was the most numerous of the ten species, and its prevalence and intensity of infestation were significantly higher on city dogs than on rural dogs (P< 0.01), whereas the converse was true for Haemaphysalis leachi (P< 0.01). Including the city of Maputo, the exact localities at which nine tick species were collected, were recorded.