Genome Sequence of a Novel Soil Actinomycete, Protaetiibacter sp. Strain SSC-01

ABSTRACT The family Microbacteriaceae represents a diverse and important group of soil bacteria in the phylum Actinobacteria. Here, we report the genome sequence of a soil Microbacteriaceae strain, Protaetiibacter sp. strain SSC-01, the second putative species of the genus. Iron acquisition and xylose metabolism are central pathways identified in the annotated genome.

E40, a novel microbial protease efficiently detoxifying gluten proteins, for the dietary management of gluten intolerance

Gluten proteins are the causative agent of Celiac Disease (CD), a life-long food intolerance characterized by an autoimmune enteropathy. Inadvertent gluten exposure is frequent even in celiac patients complying with a gluten-free diet, and the supplementation of exogenous gluten-digestive enzymes (glutenases) is indeed a promising approach to reduce the risk of dietary gluten boost. Here we describe Endopeptidase 40, a novel glutenase discovered as secreted protein from the soil actinomycete Actinoallomurus A8, and its recombinant active form produced by Streptomyces lividans TK24. E40 is resistant to pepsin and trypsin, and active in the acidic pH range 3 to 6. E40 efficiently degrades the most immunogenic 33-mer as well as the whole gliadin proteins, as demonstrated by SDS-PAGE, HPLC, LC-MS/MS, and ELISA. T lymphocytes from duodenal biopsies of celiac patients showed a strongly reduced or absent release of IFN-γ when exposed to gluten digested with E40. Data in gastrointestinal simulated conditions suggest that no toxic peptides are freed during gluten digestion by E40 into the stomach to enter the small intestine, thus counteracting the intestinal inflammatory cascade to occur in CD patients. E40 is proposed as a novel candidate in Oral Enzymatic Therapy for the dietary management of gluten toxicity.

A unique spiro-β-triazinedione-γ-hydantoin type alkaloid with antiviral activity against tobacco mosaic virus from Streptomyces gamaensis

Chemical investigation of a soil actinomycete strain Streptomyces gamaensis NEAU-Gz11 led to the isolation of three alkaloids 1–3 with antiviral activity.

A review of Spinosyns, a derivative of biological acting substances as a class of insecticides with a broad range of action against many insect pests

Spinosyns are a class of insecticides with a broad range of action against many insect pests belonging to different orders, noxious to a wide variety of agricultural crops; spinosyns were also used against insects of sanitary interest. Spinosyns are derivative of biological active substances produced by soil Actinomycete <em>Saccharopolyspora</em> <em>spinosa</em>; being of biological origin, they are considered to have a low environmental impact and they are not much aggressive against nontarget species. They act as allosteric activators of nicotinic acetylcholine receptors; thanks to their mode of action the resistance phenomena are uncommon, even few cases of resistance were recently reported. For all these reasons at present they are one of the most interesting product to be used in fighting against agriculture pests.