Background: The current version of the Brazilian Montreal Cognitive Assessment (MoCA-BR) did not have a reliable cross-cultural adaptation to Brazil-ian Portuguese. In previous stages of this study, the Alternative Version of the MoCA-BR was developed, with changes in the sections: Memory and Delayed Recall, Language and Naming. Objectives: to verify the influence of crosscultural adaptation on the performance of cognitive tools, and the accuracy of the Alternative Version of the MoCA-BR. Methods: a pilot, prospective, longitudinal and analytical study. Both versions of the test were applied in a randomized and cognitively healthy population, between 18 and 60 years, within a medium interval of 54,56 days between the questionnaires. Results: out of 104 participants, 70 were included (64.3% female, 40.2 years). The alternative version obtained superior performances in the naming domain (p < 0.001), and in the adapted sentence in the language domain (p = 0.003). There was no significant difference in the delayed recall domain. The alternative version showed good internal consistency, with a Cronbach’s alpha of 0.75. The cutoff point suggested by the study is 27 points, with sensitivity and specificity of 91.3% and 79.2%, respectively. Conclusions: Cultural factors affect the accuracy of cognitive tests, and adaptation is essential for their use in different countries.