Development and Characterization of Monoclonal Antibodies for the Detection of Fish Protein

This study developed and characterized anti-fish monoclonal antibodies (mAbs) capable of detecting fish, a major allergenic food, in processed food products to protect fish sensitized individuals. Of the three mAbs raised against crude protein extract of cooked fish muscle, mAb 8F5 exhibited a positive reaction to all 50 common food fish species tested with no cross-reactions to shellfish, land animals, or food additives. Although the ELISA results were negative against swordfish and yellowfin tuna, western blot clearly detected both after cooking. The ~36 kDa antigenic protein of mAb 8F5, which was found in all fish species, was detectable by mAb 8F5 in all of the fish samples even after prolonged heat treatment (100 °C, up to 60 min). These findings suggest that mAb 8F5 has great potential utility as a probe for the immunochemical detection of fish tissue in cooked food.

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Bioaccumulation of Polychlorinated Biphenyls and Organochlorine Pesticides in Selected Fish Species as an Indicator of the Pollution of Aquatic Resources in Vojvodina, Yugoslavia

Water Science & Technology ◽

10.2166/wst.1992.0737 ◽

1992 ◽

Vol 26 (9-11) ◽

pp. 2361-2364 ◽

Cited By ~ 5

Author(s):

M. Vojinovic-Miloradov ◽

P. Marjanovic ◽

D. Buzarov ◽

S. Pavkov ◽

Lj. Dimitrijevic ◽

...

Keyword(s):

Polychlorinated Biphenyls ◽

Organochlorine Pesticides ◽

Fish Species ◽

Food Chain ◽

Limit Of Detection ◽

Fish Tissue ◽

Aquatic Resources ◽

Fish Samples ◽

High Concentrations ◽

High Degree

Long term pollution of selected aquatic resources of Vojvodina with polychlorinated biphenyls (PCB's) and organochlorine pesticides (OCP's) has led to high degree of bioaccumulation of the above chemicals along the food chain. This paper documents the bioaccumulation in two fish species commonly found in rivers and canals of Vojvodina, common carp (Cyprinus Carpio) and pike (Exos lucinus). Samples offish, sediment and water were collected from the Danube, Sava and Tisa rivers and a fish farm “Ečka” which is supplied with water from the river Tisa and river Begej. All samples were analysed by highly sensitive capillary column GC using EC detectors. Samples were analysedfor aspectrum of compounds and are presented here as total PCB's and OCP's. The results show that the studied compounds are present in water samples at very low concentrations (PCB's in concentrations at or below the limit of detection, OCP's in concentrations below the legally allowable concentrations by Yugoslav norms). The sediment samples contain the studied compounds at significantly higher concentrations but still within the expectable range. Fish tissue contained elevated concentrations, and especially so the tissue of the top predator in the food chain, the Pike, showing that bioaccumulation has occurred. Of special concern are the extremely high concentrations of PCB's in the tissue of fish samples collected from river Sava indicating the still existing effects of pollution by PCB's in the watershed of the river Sava.

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Tissue quality of formalin adulterated nile tilapia Oreochromis Niloticus fish: A histopathological perspective

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v47i4.14074 ◽

2013 ◽

Vol 47 (4) ◽

pp. 441-444

Author(s):

SM Nomanuzzaman ◽

MN Naser ◽

M Begum ◽

ATM Abdullah ◽

TA Khan ◽

...

Keyword(s):

Muscle Tissue ◽

Oreochromis Niloticus ◽

Fish Tissue ◽

Fish Muscle ◽

Tissue Quality ◽

Muscle Sample ◽

Retail Markets ◽

Nile Tilapia Oreochromis Niloticus ◽

Fish Samples

NOTE: on 29th July 2013 the word 'aile' was changed to 'nile' in the title of this paper.In view of recent adulteration of fish in retail markets with formalin, a mock-up effort was made at laboratory condition to assess the tissue quality of formalin contaminated fish. Tilapia fish samples were intentionally despoiled with 1% formalin for 1 hour and kept exposed in open shelves for spoilage. Fish tissue quality was histopathologically tested at 0, 6, 12, 16, 20 and 24 hours intervals. From the histopathological perspective the tissue quality were found to be deteriorating gradually with time. The rate of desolation of tissue was maximum after 20 hours of sampling. The fresh (0 hour) muscle sample was found to be fairly intact. Observation of the fish samples at 6, 12 and 16 hours showed minimum degradation of muscle tissue within acceptable level. One hour exposure to 1% formalin was not found sufficient to prevent deterioration and preserve fish muscle for longer period. But this concentration of formalin and the exposure time retained the tissue quality and delayed desolation of muscle tissue for 15 to 16 hours. Bangladesh J. Sci. Ind. Res. 47(4), 441-444, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14074

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Characterization of Monoclonal Anti-human Factor VII Antibody (B101/B1) that Recognized Three-dimensional Structures near Arg at Position 79 in the First EGF-like Domain

Thrombosis and Haemostasis ◽

10.1055/s-0037-1614229 ◽

1998 ◽

Vol 79 (01) ◽

pp. 104-109 ◽

Cited By ~ 6

Author(s):

Osamu Takamiya

Keyword(s):

Monoclonal Antibodies ◽

Tissue Factor ◽

Human Factor ◽

Solid Phase ◽

Three Dimensional ◽

Coagulation Factors ◽

Factor Vii ◽

Dimensional Structure ◽

Epidermal Growth

SummaryMurine monoclonal antibodies (designated hVII-B101/B1, hVIIDC2/D4 and hVII-DC6/3D8) directed against human factor VII (FVII) were prepared and characterized, with more extensive characterization of hVII-B101/B1 that did not bind reduced FVIIa. The immunoglobulin of the three monoclonal antibodies consisted of IgG1. These antibodies did not inhibit procoagulant activities of other vitamin K-dependent coagulation factors except FVII and did not cross-react with proteins in the immunoblotting test. hVII-DC2/D4 recognized the light chain after reduction of FVIIa with 2-mercaptoethanol, and hVIIDC6/3D8 the heavy chain. hVII-B101/B1 bound FVII without Ca2+, and possessed stronger affinity for FVII in the presence of Ca2+. The Kd for hVII-B101/B1 to FVII was 1.75 x 10–10 M in the presence of 5 mM CaCl2. The antibody inhibited the binding of FVII to tissue factor in the presence of Ca2+. hVII-B101/B1 also inhibited the activation of FX by the complex of FVIIa and tissue factor in the presence of Ca2+. Furthermore, immunoblotting revealed that hVII-B101/B1 reacted with non-reduced γ-carboxyglutaminic acid (Gla)-domainless-FVII and/or FVIIa. hVII-B101/B1 showed a similar pattern to that of non-reduced proteolytic fragments of FVII by trypsin with hVII-DC2/D4 on immunoblotting test. hVII-B101/B1 reacted differently with the FVII from the dysfunctional FVII variant, FVII Shinjo, which has a substitution of Gln for Arg at residue 79 in the first epidermal growth factor (1st EGF)-like domain (Takamiya O, et al. Haemosta 25, 89-97,1995) compared with normal FVII, when used as a solid phase-antibody for ELISA by the sandwich method. hVII-B101/B1 did not react with a series of short peptide sequences near position 79 in the first EGF-like domain on the solid-phase support for epitope scanning. These results suggested that the specific epitope of the antibody, hVII-B101/B1, was located in the three-dimensional structure near position 79 in the first EGF-like domain of human FVII.

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Effects of Drying Methods on the Biochemical Composition and Organoleptic Properties of Two Freshwater Fish Species (Mormyrus rume and Labeo coubie) of Cross River Obubra, Nigeria

Journal of Marine Science Research and Oceanography ◽

10.33140/jmsro.02.01.01 ◽

2019 ◽

Vol 2 (1) ◽

Keyword(s):

Moisture Content ◽

Fish Species ◽

Nutrition Status ◽

Drying Methods ◽

Organoleptic Properties ◽

Nutrient Quality ◽

Oven Drying ◽

Fish Samples ◽

Fish And Fishery Products ◽

Mormyrus Rume

Consumers all over the world are increasingly becoming aware of the health and nutrition status of fish and fishery products. There have develop some preference for fish species and even their processing methods. This study aims to investigate the effects of two drying methods (smoking and oven drying) on the biochemical components and organoleptic properties of two less preferred food fishes abundant in the study area. The fish samples Mormyrus rume and Labeo coubie were purchased from fresh landings of fishermen at Ahaha beach. The biochemical assay of the moisture, protein, fat, carbohydrate, ash, calcium (Ca), magnesium (Mg), phosphorus (P), iron (Fe), sodium (Na) and potassium (K) were conducted in the labouratory using standard methods. The proximate composition of raw M. rume was determined as moisture 70.38%, protein 17.43%, carbohydrate 1.13%, fat 5.93% and ash 2.77% while oven dried were 18.78, 63.85, 3.37, 8.73 and 7.58% respectively. All the proximate parameters and gross energy level investigated were higher (p < 0.05) in M. rume except the moisture content of dried samples of L. coubie. The mineral content were in the order Ca < Mg < Fe < Na < P and < K in both species and were higher in M. rume except Na. The drying methods showed increase in the proximate and mineral compositions in the order raw < smoking and < oven drying except moisture content that decreased respectively is both species. Organoleptic properties revealed that dried samples (smoked and oven) were not significantly different (p < 0.05) in taste, flavour, texture and overall acceptance, however while oven dried had better colour (4.33), smoked dried taste (4.63) better. The drying methods shows concentration of the required nutrients in human diets and were also found to be most palatable by the panellist. To improve fish nutrient quality smoke and oven drying should be encourage.

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