Auxiliary function-based algorithm for blind extraction of a moving speaker

In this paper, we propose a novel algorithm for blind source extraction (BSE) of a moving acoustic source recorded by multiple microphones. The algorithm is based on independent vector extraction (IVE) where the contrast function is optimized using the auxiliary function-based technique and where the recently proposed constant separating vector (CSV) mixing model is assumed. CSV allows for movements of the extracted source within the analyzed batch of recordings. We provide a practical explanation of how the CSV model works when extracting a moving acoustic source. Then, the proposed algorithm is experimentally verified on the task of blind extraction of a moving speaker. The algorithm is compared with state-of-the-art blind methods and with an adaptive BSE algorithm which processes data in a sequential manner. The results confirm that the proposed algorithm can extract the moving speaker better than the BSE methods based on the conventional mixing model and that it achieves improved extraction accuracy than the adaptive method.

An international, inter-laboratory ring trial confirms the feasibility of an open source, extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples

RT-qPCR is used world-wide to test and trace the spread of SARS-CoV-2. Extraction-less or direct RT-PCR is an open-access qualitative method for SARS-CoV-2 detection from nasopharyngeal (NP) or oral pharyngeal (OP) samples with the potential to generate actionable data more quickly, at a lower cost, and with fewer experimental resources than full RT-qPCR. This study engaged ten global testing sites, including laboratories currently experiencing testing limitations due to reagent or equipment shortages, in an international inter-laboratory ring trial. Participating labs were provided a common protocol, common reagents, aliquots of identical pooled clinical samples and purified nucleic acids, and used their existing in-house equipment. We observed 100% concordance across labs in the correct identification of all positive and negative samples, with highly similar Ct values observed. The test also performed well when applied to locally collected patient NP samples, provided the viral transport media did not contain charcoal or guanidine, both of which appeared to potently inhibit the RT-PCR reaction. Our results suggest that open access, direct RT-PCR assays are a feasible option for more efficient COVID-19 testing as demanded by the continuing pandemic.

Cost-effective and sensitive anthocyanin-based paper sensors for rapid ammonia detection in aqueous solutions

In this work, we studied the effect of different factors such as anthocyanin source, extraction technique, and extracting solvent on the sensitivity and optical visibility of anthocyanin-based paper sensors.