Kunyima Method Validity in Sesame Seeds Oil Extraction "Democratic Republic of Congo"

Background: The very rich vegetable patrimony of Democratic Republic of Congo (DRC) demands a local abundant production of its fruits, roots and seeds by means of appropriate technology to relieve somewhat the problem of population health because indeed those fruits, seeds, roots and leaves are reputed to have very excellent virtues in various fields such as medicine, pharmacology, cosmetic products, food and so forth. Prior to this work papers have been published on Gourd seeds oil and Moringa seeds oil extractions in order to size a continuous stirrer pilot tank of extraction. KUNYIMA method has been successfully used and is still successfully used in this present article concerning sesame (Sesamum indicum L.) seeds oil extraction in dilute medium. Aim and Objective: In this paper it can be seen the reasoning leading to KUNYIMA method is relevant and can be used to size commercial tank giving thus home rational technology. Methodology: The Soxhlet extraction of oil from sesame (Sesamum indicum L.) seeds has been performed in dilute medium using petroleum ether as solvent. Results: The obtained results are satisfactory and are perfectly in agreement with the proposed model. Commercial tank construction on rational calculation is now possible. Conclusion: KUNYIMA method once more is valid and allows to calculate the sizing factor needful to determine the reactor optimal volume.

Role of cytoskeleton in volume regulation of rabbit proximal tubule in dilute medium

When proximal tubules are immersed in hypotonic medium, they quickly swell to a peak volume. In a second, slower phase, termed volume regulatory decrease (VRD), they shrink as K, anion, and water leave the cells. We investigated the role of the cytoskeleton during this biphasic hypotonic volume regulatory response. Isolated, collapsed rabbit proximal convoluted tubules (PCT) were crimped tightly between two pipettes, and their volume was assessed optically. PCT volume increased to a peak 70-80% above baseline on sudden immersion in dilute medium (150 mosmol/kgH2O). After completing VRD, control tubules had regulated their volume 73 +/- 2% back toward baseline. Tubules exposed to the microtubule inhibitor vincristine (5 microM) regulated 75 +/- 2%. Tubules exposed to the microfilament inhibitor cytochalasin B (50 microM) regulated less (57 +/- 5%), and tubules exposed to both inhibitors regulated only 39 +/- 3% (P less than 0.01 vs. control). Hypotonic VRD was unimpaired in PCT loaded with NaCl by prior exposure to ouabain but was significantly reduced by cytochalasin B. We conclude that VRD is not cation specific and that intact microtubules and microfilaments play a synergistic role in the VRD of rabbit PCT in hyposmotic medium.

Effect of barium on cell volume regulation in rabbit proximal straight tubules

Rabbit proximal straight tubules swell abruptly when exposed to hypotonic medium but then shrink in a few minutes as they approach their base-line volume following loss of solute and water. Potassium, the major intracellular cation, as well as sodium, is lost during this process. In the present experiments, we studied hypotonic cell volume regulation in the presence of barium, an agent reported to decrease potassium permeability. Exposure to BaCl2 significantly prolonged hypotonic volume recovery in a dose-dependent manner. Tubules depleted of potassium and loaded with sodium chloride by exposure to 10(-4) M ouabain for 1 h swelled osmometrically and subsequently volume regulated in dilute medium. Volume regulation in such tubules is a consequence of transbasement membrane hydrostatic forces. By contrast, tubules similarly loaded with sodium, but also exposed to 10(-3) M BaCl2, volume regulated only minimally in dilute medium, suggesting BaCl2 might also affect sodium movement. However, hypotonic volume regulation was restored in sodium-loaded BaCl2-treated tubules when cells were more effectively depleted of potassium by incubation in 0-mM potassium medium. We conclude that barium retards hypotonic volume regulation primarily because of its effect on potassium movement.

Osmoregulation in Some Freshwater Prawns

I. A freshwater prawn, Palaemonetes antennarius, from Italy, is shown to have a different level of salt concentration in the blood and urine compared with that of the brackish water P. varians and Palaemon longirostris in Britain. 2. Although this prawn lives in fresh water the urine is isosmotic with the blood and is produced at a rate of about 2% body weight/hr. 3. An uptake of salte from a very dilute medium has been demonstrated in saltdepleted animals