Demonstration of interleukin 8 in serum samples of patients with localized scleroderma

Background. Currently available biomarkers are not specific and sensitive enough for early detection of bacterial infection in patients with cancer who have febrile neutropenia. The objective of this study was to assess diagnostic accuracy of interleukin-6 (IL-6), interleukin-8 (IL-8) and procalcitonin (PCT) in the identification of Gram-negative bacteremia at the beginning of a febrile episode in pediatric oncology patients with acute lymphoblastic leukemia (ALL). Methods. A total of 40 episodes of febrile neutropenia in 27 childhood cancer patients were enrolled in this study. Serum samples were collected at presentation after confirmation of febrile neutropenia and analyzed according to the recommendations of manufacturers. Patients were classified into Gram-negative bacteremia (GNB) and fever of unknown origin (FUO) groups. Results. The median concentration of IL-6, IL-8 and PCT were higher in the GNB group compared to the FUO group (65.4 vs. 409.0 pg/ml, P = 0.0025; 166.0 vs. 883.0 pg/ml, P = 0.0002; 0.27 vs. 0.44 ng/ml, P = 0.0169, respectively). The areas under the curves (AUCs) for both IL-6 and IL-8 were 0.94 and 0.93, respectively, indicating that these cytokines discriminated patients with Gram-negative bacteremia with excellent accuracy, whereas PCT had lower diagnostic accuracy (AUC = 0.76). Conclusions. IL-6 and IL-8 evaluation might be used as an additional diagnostic tool for the prediction of Gram-negative bacteremia in pediatric patients with ALL during febrile neutropenia episodes.

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Intraperitoneal Interleukin-8 and Neutrophil Influx in the Initial Phase of a Capd Peritonitis

Peritoneal Dialysis International ◽

10.1177/089686089601600412 ◽

1996 ◽

Vol 16 (4) ◽

pp. 385-392 ◽

Cited By ~ 18

Author(s):

Michiel G.H. Betjes ◽

Carolien E. Visser ◽

Desiree Zemel ◽

Cornelis W. Tuk ◽

Dirk G. Struijk ◽

...

Keyword(s):

Initial Phase ◽

Interleukin 8 ◽

Neutrophil Chemotaxis ◽

Serum Samples ◽

Differential Cell Count ◽

Neutrophil Influx ◽

Differential Cell ◽

Control Study ◽

Number Of Cells

Objective To investigate whether or not a change in dialysate interleukin-8 (IL-8) concentration precedes the onset of clinically overt peritonitis and is significant in the recruitment of granulocytes during continuous ambulatory peritoneal dialysis (CAPD)related peritonitis. Design CAPD patients stored their overnight effluent at 4°C, which was routinely thrown away after 2 days. If peritonitis developed, patients delivered their effluent of the preceding two nights and the peritonitis effluent for analysis. A control study was performed 1 to 3 months after recovery. Dialysate samples were analyzed for number of cells, differential cell count, IL-8 and elastase concentrations, and their neutrophil chemoattractive capacity. In addition, serum samples during peritonitis were analyzed for IL-8 concentrations. Results Ten peritonitis episodes in 7 patients were analyzed. Numbers of neutrophils and levels of dialysate IL-8 and elastase started to increase 4 to 12 hours before the first peritonitis effluent. The dialysate/serum IL-8 ratio was 423.5 during peritonitis and 7.0 in the postperitonitis controls. There was a significant correlation between the number of neutrophils and IL-8 concentration in the dialysate. The in vitro neutrophil chemotaxis was increased toward the peritonitis effluents, as compared to control effluents. Incubation of the peritonitis effluents with anti-IL-8 monoclonal antibody blocked the increase in neutrophil chemotaxis above controllevels by an average of 26.7%. Conclusion IL-8 is produced in the peritoneal cavity during CAPD treatment and may mediate part of the neutrophil recruitment and degranulation in the initial phase of a CAPD peritonitis.

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