Intracerebral Inoculation of Experimental Animals With Brain Tissue From Patients With Schizophrenia

The minute microörganism cultivated from poliomyelitic tissues survives and maintains its pathogenicity in cultures for more than one year. Upon inoculation into monkeys poliomyelitis may fail to appear upon the first injection and yet follow from the effects of successive injections of the culture. Inoculations of cultures into monkeys which fail to produce paralysis may fail also to induce resistance or immunity. In this respect the action of the cultures resembles that of the virus as contained in infected nervous tissues. The lesions occurring in the spinal cord, medulla, and intervertebral ganglia of the monkeys which respond to the several inoculations of the cultures are identical with those present in the nervous organs of the animals responding to injection of the ordinary virus. Glycerinated nervous tissues derived from the monkeys responding to several injections of the cultures transmit experimental poliomyelitis to monkeys upon intracerebral inoculation. The microörganism inoculated may be recovered in cultures from the monkeys which develop poliomyelitis; but cultivation from the brain tissue is attended with the usual difficulties surrounding the obtaining of the initial growth. The microörganism cultivated from poliomyelitic tissues is adapted with difficulty to saprophytic conditions of multiplication, but once adapted growth readily takes place upon suitable media. When, however, as a result of inoculation into monkeys, the parasitic propensities of the microörganism are restored, it again displays the marked fastidiousness to artificial conditions of multiplication present at the original isolation. The experiments reported in this paper afford additional strong evidence in support of the view already expressed, that this microorganism bears an etiological relationship to epidemic poliomyelitis in the human subject and to experimental poliomyelitis in the monkey.

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Scrapie in a Dorset Down ram. A confirmation of the histological diagnosis by means of intracerebral inoculation of mice with formol fixed brain tissue

Veterinary Record ◽

10.1136/vr.77.40.1178 ◽

1965 ◽

Vol 77 (40) ◽

pp. 1178-1179 ◽

Cited By ~ 6

Author(s):

I. Zlotnik ◽

J. Stamp

Keyword(s):

Brain Tissue ◽

Histological Diagnosis ◽

Intracerebral Inoculation

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AN IMPROVED INSTRUMENT FOR THE INTRACEREBRAL INOCULATION OF EXPERIMENTAL ANIMALS

Science ◽

10.1126/science.86.2242.567 ◽

1937 ◽

Vol 86 (2242) ◽

pp. 567-568

Author(s):

J. T. Syverton ◽

G. P. Berry

Keyword(s):

Experimental Animals ◽

Intracerebral Inoculation

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Regional distribution of opiate alkaloids in experimental animals' brain tissue and blood

Acta veterinaria ◽

10.2298/avb1203137d ◽

2012 ◽

Vol 62 (2-3) ◽

pp. 137-149 ◽

Cited By ~ 1

Author(s):

Maja Djurendic-Brenesel ◽

V. Pilija ◽

S. Cvjeticanin ◽

Vesna Ivetic ◽

Neda Mimica-Dukic

Keyword(s):

Brain Tissue ◽

Regional Distribution ◽

Experimental Animals

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Distribution of opiate alkaloids in brain tissue of experimental animals

Interdisciplinary Toxicology ◽

10.2478/v10102-012-0029-y ◽

2012 ◽

Vol 5 (4) ◽

pp. 173-178 ◽

Cited By ~ 4

Author(s):

Maja Djurendic-Brenesel ◽

Vladimir Pilija ◽

Neda Mimica-Dukic ◽

Branislav Budakov ◽

Stanko Cvjeticanin

Keyword(s):

Basal Ganglia ◽

Brain Tissue ◽

Regional Distribution ◽

Opiate Receptors ◽

Brain Regions ◽

Gas Chromatography Mass Spectrometry ◽

High Concentration ◽

Experimental Animals ◽

Female Wistar Rats ◽

The Brain

ABSTRACT The present study examined regional distribution of opiate alkaloids from seized heroin in brain regions of experimental animals in order to select parts with the highest content of opiates. Their analysis should contribute to resolve causes of death due to heroin intake. The tests were performed at different time periods (5, 15, 45 and 120 min) after male and female Wistar rats were treated with seized heroin. Opiate alkaloids (codeine, morphine, acetylcodeine, 6-acetylmorphine and 3,6-diacetylmorphine) were quantitatively determined in brain regions known for their high concentration of μ-opiate receptors: cortex, brainstem, amygdala and basal ganglia, by using gas chromatography-mass spectrometry (GC-MS). The highest content of opiate alkaloids in the brain tissue of female animals was found 15 min and in male animals 45 min after treatment. The highest content of opiates was determined in the basal ganglia of the animals of both genders, indicating that this part of brain tissue presents a reliable sample for identifying and assessing contents of opiates after heroin intake.

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Analytical Electron Microscopy (AEM) of Meningeal Cells Exposed to Particulate Cobalt During Studies of Experimental Epilepsy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053590 ◽

1982 ◽

Vol 40 ◽

pp. 186-187

Author(s):

R.G. Frederickson ◽

R.G. Ulrich ◽

J.L. Culberson

Keyword(s):

Electron Microscopy ◽

Analytical Electron Microscopy ◽

Experimental Epilepsy ◽

Metallic Cobalt ◽

Experimental Animals ◽

X Ray ◽

Brain Surface ◽

Meningeal Cells ◽

Analytical Electron ◽

The Brain

Metallic cobalt acts as an epileptogenic agent when placed on the brain surface of some experimental animals. The mechanism by which this substance produces abnormal neuronal discharge is unknown. One potentially useful approach to this problem is to study the cellular and extracellular distribution of elemental cobalt in the meninges and adjacent cerebral cortex. Since it is possible to demonstrate the morphological localization and distribution of heavy metals, such as cobalt, by correlative x-ray analysis and electron microscopy (i.e., by AEM), we are using AEM to locate and identify elemental cobalt in phagocytic meningeal cells of young 80-day postnatal opossums following a subdural injection of cobalt particles.

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Ultrastructure and analytical microprobe analysis of simian lung mite pigments

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143249 ◽

1986 ◽

Vol 44 ◽

pp. 324-325

Author(s):

R. W. Cole ◽

J. C. Kim

Keyword(s):

Biomedical Research ◽

Tissue Damage ◽

Microprobe Analysis ◽

Old World Monkeys ◽

Old World ◽

Experimental Animals ◽

The Past ◽

Lung Mite ◽

Mite Infestations ◽

Cardiopulmonary System

In recent years, non-human primates have become indispensable as experimental animals in many fields of biomedical research. Pharmaceutical and related industries alone use about 2000,000 primates a year. Respiratory mite infestations in lungs of old world monkeys are of particular concern because the resulting tissue damage can directly effect experimental results, especially in those studies involving the cardiopulmonary system. There has been increasing documentation of primate parasitology in the past twenty years.

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