Reliable and durable Golgi staining of brain tissue from human autopsies and experimental animals

Classical and modern neuroanatomical techniques are applied to an analysis of the neuroanatomical basis of schizophrenia using postmortem brain tissue from the Maryland Brain Collection. Techniques for Golgi staining of cell bodies and dendritic arbors, axonal tract tracing with the carbocyanine dye, DiI and ultrastructural analyses will be used to study brain tissue with postmortem times of less than 6 hours. Although these techniques are routinely used in animal models, they are rarely used for studying human brain tissue. These techniques will allow us to characterize neuronal architecture in normal and diseased brains.

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Intracerebral Inoculation of Experimental Animals With Brain Tissue From Patients With Schizophrenia

Archives of General Psychiatry ◽

10.1001/archpsyc.1988.01800310056007 ◽

1988 ◽

Vol 45 (7) ◽

pp. 648 ◽

Cited By ~ 8

Author(s):

Charles A. Kaufmann

Keyword(s):

Brain Tissue ◽

Experimental Animals ◽

Intracerebral Inoculation

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Regional distribution of opiate alkaloids in experimental animals' brain tissue and blood

Acta veterinaria ◽

10.2298/avb1203137d ◽

2012 ◽

Vol 62 (2-3) ◽

pp. 137-149 ◽

Cited By ~ 1

Author(s):

Maja Djurendic-Brenesel ◽

V. Pilija ◽

S. Cvjeticanin ◽

Vesna Ivetic ◽

Neda Mimica-Dukic

Keyword(s):

Brain Tissue ◽

Regional Distribution ◽

Experimental Animals

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Distribution of opiate alkaloids in brain tissue of experimental animals

Interdisciplinary Toxicology ◽

10.2478/v10102-012-0029-y ◽

2012 ◽

Vol 5 (4) ◽

pp. 173-178 ◽

Cited By ~ 4

Author(s):

Maja Djurendic-Brenesel ◽

Vladimir Pilija ◽

Neda Mimica-Dukic ◽

Branislav Budakov ◽

Stanko Cvjeticanin

Keyword(s):

Basal Ganglia ◽

Brain Tissue ◽

Regional Distribution ◽

Opiate Receptors ◽

Brain Regions ◽

Gas Chromatography Mass Spectrometry ◽

High Concentration ◽

Experimental Animals ◽

Female Wistar Rats ◽

The Brain

ABSTRACT The present study examined regional distribution of opiate alkaloids from seized heroin in brain regions of experimental animals in order to select parts with the highest content of opiates. Their analysis should contribute to resolve causes of death due to heroin intake. The tests were performed at different time periods (5, 15, 45 and 120 min) after male and female Wistar rats were treated with seized heroin. Opiate alkaloids (codeine, morphine, acetylcodeine, 6-acetylmorphine and 3,6-diacetylmorphine) were quantitatively determined in brain regions known for their high concentration of μ-opiate receptors: cortex, brainstem, amygdala and basal ganglia, by using gas chromatography-mass spectrometry (GC-MS). The highest content of opiate alkaloids in the brain tissue of female animals was found 15 min and in male animals 45 min after treatment. The highest content of opiates was determined in the basal ganglia of the animals of both genders, indicating that this part of brain tissue presents a reliable sample for identifying and assessing contents of opiates after heroin intake.

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Analytical Electron Microscopy (AEM) of Meningeal Cells Exposed to Particulate Cobalt During Studies of Experimental Epilepsy

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053590 ◽

1982 ◽

Vol 40 ◽

pp. 186-187

Author(s):

R.G. Frederickson ◽

R.G. Ulrich ◽

J.L. Culberson

Keyword(s):

Electron Microscopy ◽

Analytical Electron Microscopy ◽

Experimental Epilepsy ◽

Metallic Cobalt ◽

Experimental Animals ◽

X Ray ◽

Brain Surface ◽

Meningeal Cells ◽

Analytical Electron ◽

The Brain

Metallic cobalt acts as an epileptogenic agent when placed on the brain surface of some experimental animals. The mechanism by which this substance produces abnormal neuronal discharge is unknown. One potentially useful approach to this problem is to study the cellular and extracellular distribution of elemental cobalt in the meninges and adjacent cerebral cortex. Since it is possible to demonstrate the morphological localization and distribution of heavy metals, such as cobalt, by correlative x-ray analysis and electron microscopy (i.e., by AEM), we are using AEM to locate and identify elemental cobalt in phagocytic meningeal cells of young 80-day postnatal opossums following a subdural injection of cobalt particles.

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Ultrastructure and analytical microprobe analysis of simian lung mite pigments

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100143249 ◽

1986 ◽

Vol 44 ◽

pp. 324-325

Author(s):

R. W. Cole ◽

J. C. Kim

Keyword(s):

Biomedical Research ◽

Tissue Damage ◽

Microprobe Analysis ◽

Old World Monkeys ◽

Old World ◽

Experimental Animals ◽

The Past ◽

Lung Mite ◽

Mite Infestations ◽

Cardiopulmonary System

In recent years, non-human primates have become indispensable as experimental animals in many fields of biomedical research. Pharmaceutical and related industries alone use about 2000,000 primates a year. Respiratory mite infestations in lungs of old world monkeys are of particular concern because the resulting tissue damage can directly effect experimental results, especially in those studies involving the cardiopulmonary system. There has been increasing documentation of primate parasitology in the past twenty years.

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