scholarly journals Regional distribution of opiate alkaloids in experimental animals' brain tissue and blood

2012 ◽  
Vol 62 (2-3) ◽  
pp. 137-149 ◽  
Author(s):  
Maja Djurendic-Brenesel ◽  
V. Pilija ◽  
S. Cvjeticanin ◽  
Vesna Ivetic ◽  
Neda Mimica-Dukic
Keyword(s):  
Brain Tissue ◽  
Regional Distribution ◽  
Experimental Animals

scholarly journals Distribution of opiate alkaloids in brain tissue of experimental animals

2012 ◽  
Vol 5 (4) ◽  
pp. 173-178 ◽  
Author(s):  
Maja Djurendic-Brenesel ◽  
Vladimir Pilija ◽  
Neda Mimica-Dukic ◽  
Branislav Budakov ◽  
Stanko Cvjeticanin
Keyword(s):  
Basal Ganglia ◽  
Brain Tissue ◽  
Regional Distribution ◽  
Opiate Receptors ◽  
Brain Regions ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Experimental Animals ◽  
Female Wistar Rats ◽  
The Brain

ABSTRACT The present study examined regional distribution of opiate alkaloids from seized heroin in brain regions of experimental animals in order to select parts with the highest content of opiates. Their analysis should contribute to resolve causes of death due to heroin intake. The tests were performed at different time periods (5, 15, 45 and 120 min) after male and female Wistar rats were treated with seized heroin. Opiate alkaloids (codeine, morphine, acetylcodeine, 6-acetylmorphine and 3,6-diacetylmorphine) were quantitatively determined in brain regions known for their high concentration of μ-opiate receptors: cortex, brainstem, amygdala and basal ganglia, by using gas chromatography-mass spectrometry (GC-MS). The highest content of opiate alkaloids in the brain tissue of female animals was found 15 min and in male animals 45 min after treatment. The highest content of opiates was determined in the basal ganglia of the animals of both genders, indicating that this part of brain tissue presents a reliable sample for identifying and assessing contents of opiates after heroin intake.

scholarly journals Reliable and durable Golgi staining of brain tissue from human autopsies and experimental animals

2014 ◽  
Vol 230 ◽  
pp. 20-29 ◽  
Author(s):  
Gorazd B. Rosoklija ◽  
Vladimir M. Petrushevski ◽  
Aleksandar Stankov ◽  
Ani Dika ◽  
Zlatko Jakovski ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Experimental Animals ◽  
Golgi Staining

Ouabain-Induced Seizures in Rats: Modification by Melatonin and Melanocyte-Stimulating Hormone

1973 ◽  
Vol 51 (8) ◽  
pp. 572-578 ◽  
Author(s):  
K. Izumi ◽  
J. Donaldson ◽  
J. Minnich ◽  
A. Barbeau
Keyword(s):  
Seizure Activity ◽  
Regional Distribution ◽  
Suppressive Effect ◽  
Young Female ◽  
Female Rats ◽  
High Accumulation ◽  
Experimental Animals ◽  
Melanocyte Stimulating Hormone ◽  
The Brain ◽  
Stimulating Hormone

The effects of melatonin and synthetic alpha-melanocyte-stimulating hormone (MSH) on ouabain-induced seizures in experimental animals were determined. Fifteen micrograms of melatonin or ten micrograms of α-MSH (dissolved in 2% ethanol – 0.85% saline) were administered intraventricularly to young female rats. The administration of melatonin was found to provide a protective effect against the seizures. The maximum suppressive effect against ouabain seizures was obtained when melatonin was given 1 min prior to the injection of ouabain. In contrast, the injection of α-MSH aggravated seizure activity elicited by 3 μg of ouabain.During experiments designed to determine the regional distribution in the brain of intraventricularly administered 3H-melatonin, high accumulation of the radioactive compound was found in the medulla oblongata – pons, the hypothalamus, and the hippocampus. The lowest activity was in the cerebral cortex.

scholarly journals Developmental and regional distribution of aspartoacylase in rat brain tissue

2008 ◽  
Vol 79 (1) ◽  
pp. 211-220 ◽  
Author(s):  
Kishore K. Bhakoo ◽  
Tim J. Craig ◽  
Peter Styles
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Regional Distribution ◽  
Rat Brain Tissue

scholarly journals Quantification of Endogenous Brain Tissue Displacement Imaging by Radiofrequency Ultrasound

Diagnostics ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 57 ◽  
Author(s):  
Rytis Jurkonis ◽  
Monika Makūnaitė ◽  
Mindaugas Baranauskas ◽  
Arūnas Lukoševičius ◽  
Andrius Sakalauskas ◽  
...  
Keyword(s):  
Brain Tissue ◽  
Tissue Characterization ◽  
Pearson Correlation ◽  
Ultrasonic Transducer ◽  
Regional Distribution ◽  
Diagnostic System ◽  
Preclinical Study ◽  
Elderly Subjects ◽  
Healthy Elderly

The purpose of this paper is a quantification of displacement parameters used in the imaging of brain tissue endogenous motion using ultrasonic radiofrequency (RF) signals. In a preclinical study, an ultrasonic diagnostic system with RF output was equipped with dedicated signal processing software and subject head–ultrasonic transducer stabilization. This allowed the use of RF scanning frames for the calculation of micrometer-range displacements, excluding sonographer-induced motions. Analysis of quantitative displacement estimates in dynamical phantom experiments showed that displacements of 55 µm down to 2 µm were quantified as confident according to Pearson correlation between signal fragments (minimum p ≤ 0.001). The same algorithm and scanning hardware were used in experiments and clinical imaging which allows translating phantom results to Alzheimer’s disease patients and healthy elderly subjects as examples. The confident quantitative displacement waveforms of six in vivo heart-cycle episodes ranged from 8 µm up to 263 µm (Pearson correlation p ≤ 0.01). Displacement time sequences showed promising possibilities to evaluate the morphology of endogenous displacement signals at each point of the scanning plane, while displacement maps—regional distribution of displacement parameters—were essential for tissue characterization.

Analytical Electron Microscopy (AEM) of Meningeal Cells Exposed to Particulate Cobalt During Studies of Experimental Epilepsy

1982 ◽  
Vol 40 ◽  
pp. 186-187
Author(s):  
R.G. Frederickson ◽  
R.G. Ulrich ◽  
J.L. Culberson
Keyword(s):  
Electron Microscopy ◽  
Analytical Electron Microscopy ◽  
Experimental Epilepsy ◽  
Metallic Cobalt ◽  
Experimental Animals ◽  
X Ray ◽  
Brain Surface ◽  
Meningeal Cells ◽  
Analytical Electron ◽  
The Brain

Metallic cobalt acts as an epileptogenic agent when placed on the brain surface of some experimental animals. The mechanism by which this substance produces abnormal neuronal discharge is unknown. One potentially useful approach to this problem is to study the cellular and extracellular distribution of elemental cobalt in the meninges and adjacent cerebral cortex. Since it is possible to demonstrate the morphological localization and distribution of heavy metals, such as cobalt, by correlative x-ray analysis and electron microscopy (i.e., by AEM), we are using AEM to locate and identify elemental cobalt in phagocytic meningeal cells of young 80-day postnatal opossums following a subdural injection of cobalt particles.

Ultrastructure and analytical microprobe analysis of simian lung mite pigments

1986 ◽  
Vol 44 ◽  
pp. 324-325
Author(s):  
R. W. Cole ◽  
J. C. Kim
Keyword(s):  
Biomedical Research ◽  
Tissue Damage ◽  
Microprobe Analysis ◽  
Old World Monkeys ◽  
Old World ◽  
Experimental Animals ◽  
The Past ◽  
Lung Mite ◽  
Mite Infestations ◽  
Cardiopulmonary System

In recent years, non-human primates have become indispensable as experimental animals in many fields of biomedical research. Pharmaceutical and related industries alone use about 2000,000 primates a year. Respiratory mite infestations in lungs of old world monkeys are of particular concern because the resulting tissue damage can directly effect experimental results, especially in those studies involving the cardiopulmonary system. There has been increasing documentation of primate parasitology in the past twenty years.

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