Sequence-Based Prediction of Residue-Level Properties in Proteins

2009 ◽  
pp. 157-187
Author(s):  
Shandar Ahmad ◽  
Yemlembam Hemjit Singh ◽  
Marcos J. Arazo-Bravo ◽  
Akinori Sarai
Keyword(s):  
2017 ◽  
Vol 2 (2) ◽  
pp. 173
Author(s):  
Henny Nurhasnawati ◽  
Siti Jubaidah ◽  
Novita Elfia

Tetracycline HCl is one of the most commonly used antibiotics in fish farming that aims to control diseases caused by bacteria. The purpose of this study was to determine the presence and the level of tetracycline HCl antibiotic residue in freshwater fishes sold at the Segiri traditional market. The method used in this research was a standard addition ultraviolet spectrophotometry. The results showed that residue level of tetracycline HCl in freshwater fish is 192,067 μg/g – 257,409 μg/g. These result was far exceeded the maximum residue level of tetracycline class antibiotics in meat and dairy based on SNI 01-6366-2000 that limit the residue not higher than 0.1 μg/g


2021 ◽  
Author(s):  
David Gloriam ◽  
Alexander Hauser ◽  
Albert Kooistra ◽  
Christian Munk ◽  
M. Madan Babu

Abstract Two-thirds of human hormones and one-third of clinical drugs activate ~350 G protein-coupled receptors belonging to four classes: A, B1, C and F. Whereas a model of activation has been described for class A, very little is known about the activation of the other classes which differ by being activated by endogenous ligands bound mainly or entirely extracellularly. Here, we show that although they use the same structural scaffold and share several helix macroswitches, the GPCR classes differ in their microswitch residue positions and contacts. We present molecular mechanistic maps of activation for each GPCR class and new methods for contact analysis applicable for any functional determinants. This is the first superfamily residue-level rationale for conformational selection and allosteric communication by ligands and G proteins laying the foundation for receptor-function studies and drugs with the desired modality.


EFSA Journal ◽  
2017 ◽  
Vol 15 (3) ◽  
Author(s):  
◽  
Alba Brancato ◽  
Daniela Brocca ◽  
Chloé De Lentdecker ◽  
Zoltan Erdos ◽  
...  

2008 ◽  
Vol 26 (3) ◽  
pp. 321-324 ◽  
Author(s):  
Gilberto C de Baptista ◽  
Luiz Roberto P Trevizan ◽  
André A Franco ◽  
Renato A da Silva

Pesticide residues, especially in vegetables and fruits, are a reason for concern with respect to the health of consumers and workers that need to return to areas that have been treated with these agrochemicals. The objectives of this experiment were to (a) study the behavior of deltamethrin residues from different formulations on cucumber fruits; and (b) correlate insecticide contents in the fruits with pickleworm control. Treatments were as follows: (a) control; (b) three 30 mL applications of deltamethrin 25 EC 100 L-1 water (emulsifiable concentrate formulation); (c) three 7.5 mL applications of deltamethrin 100 EC 100 L-1 water (emulsifiable concentrate); and (d) three 3.75 mL applications of deltamethrin 200 CS 100 L-1 water (concentrate suspension). Samples were taken at (-1), zero, 1; 3; 5; 7, and 14 days after the last application. Residues were determined by the gas chromatography technique, using an electron capture detector. In order to evaluate insecticide effectiveness, six infestation surveys were conducted during the same sampling times. Although at low levels, the deltamethrin residues in the fruits were above the maximum residue level (MRL = 0.03 mg kg-1), even one day after the end of the safety interval (2 days), and were higher for the CS formulation. The insecticide was effective to control the pest during the entire evaluation period.


1980 ◽  
Vol 20 (105) ◽  
pp. 504
Author(s):  
JR Hargreaves ◽  
KJ Melksham

Analysis of diazinon residues in cabbages up to 14 days after the final spray application, showed an initial period of rapid decay followed by a period of slower decay, which approximates to first order kinetics. The Australian maximum residue level of 0.7 mg kg-1 diazinon for the whole cabbage was reached in 2-3 days in summer and 7-8 days in winter. The recommended Australia 14-day withholding period, combined with marketing of hearts alone, conformed to Japanese marketing requirements of 0.1 mg kg-1.


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