Chain terminator sequencing procedure

This paper examines the feasibility of allowing the texts that are used in a course to sequence the target vocabulary of a course, in this paper the vocabulary of academic study as represented by the University Word List. It was found that such an approach would only allow the learners to meet a little over half of the 836 word vocabulary and that a three-step sequencing procedure would be needed to effectively meet all of the wanted vocabulary. The three steps would involve (1) using adapted texts to gradually introduce the very common academic words, (2) using unsimplified texts to meet the next 200-300 words, and (3) relying on extensive reading and planned decontextualised learning to meet the remaining lower frequency items.

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Using texts to sequence the introduction of new vocabulary in an eap course

10.26686/wgtn.12560384 ◽

2020 ◽

Author(s):

D Worthington ◽

Paul Nation

Keyword(s):

Word List ◽

Extensive Reading ◽

The University ◽

Sequencing Procedure ◽

Academic Study

This paper examines the feasibility of allowing the texts that are used in a course to sequence the target vocabulary of a course, in this paper the vocabulary of academic study as represented by the University Word List. It was found that such an approach would only allow the learners to meet a little over half of the 836 word vocabulary and that a three-step sequencing procedure would be needed to effectively meet all of the wanted vocabulary. The three steps would involve (1) using adapted texts to gradually introduce the very common academic words, (2) using unsimplified texts to meet the next 200-300 words, and (3) relying on extensive reading and planned decontextualised learning to meet the remaining lower frequency items.

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Detection of DNA sites damaged by 1-(4-amino-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU) using a DNA sequencing procedure

Cell Biology International Reports ◽

10.1016/0309-1651(87)90158-5 ◽

1987 ◽

Vol 11 (11) ◽

pp. 797-802 ◽

Cited By ~ 2

Author(s):

K MINEURA ◽

S FUSHIMI ◽

Y ITOH ◽

M KOWADA

Keyword(s):

Dna Sequencing ◽

Sequencing Procedure

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Molecular characterization of glucose-6-phosphate dehydrogenase (G6PD) deficiency in patients of Chinese descent and identification of new base substitutions in the human G6PD gene

Blood ◽

10.1182/blood.v81.8.2150.2150 ◽

1993 ◽

Vol 81 (8) ◽

pp. 2150-2154 ◽

Cited By ~ 40

Author(s):

DT Chiu ◽

L Zuo ◽

L Chao ◽

E Chen ◽

E Louie ◽

...

Keyword(s):

G6pd Deficiency ◽

Point Mutations ◽

Nucleotide Substitutions ◽

New Findings ◽

G6pd Gene ◽

High Prevalence ◽

Chinese Descent ◽

Glucose 6 Phosphate Dehydrogenase ◽

Sequencing Procedure

Abstract The underlying DNA changes associated with glucose-6-phosphate dehydrogenase (G6PD)-deficient Asians have not been extensively investigated. To fill this gap, we sequenced the G6PD gene of 43 G6PD- deficient Chinese whose G6PD was well characterized biochemically. DNA samples were obtained from peripheral blood of these individuals for sequencing using a direct polymerase chain reaction (PCR) sequencing procedure. From these 43 samples, we have identified five different types of nucleotide substitutions in the G6PD gene: at cDNA 1388 from G to A (Arg to His); at cDNA 1376 from G to T (Arg to Leu); at cDNA 1024 from C to T (Leu to Phe); at cDNA 392 from G to T (Gly to Val); at cDNA 95 from A to G (His to Arg). These five nucleotide substitutions account for over 83% of our 43 G6PD-deficient samples and these substitutions have not been reported in non-Asians. The substitutions found at cDNA 392 and cDNA 1024 are new findings. The substitutions at cDNA 1376 and 1388 account for over 50% of the 43 samples examined indicating a high prevalence of these two alleles among G6PD-deficient Chinese. Our findings add support to the notion that diverse point mutations may account largely for much of the phenotypic heterogeneity of G6PD deficiency.

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A Rapid DNA Sequencing Procedure: Unidirectional Deletion of DNA Fragments and Use of Reverse Transcriptase in Sequencing Reactions

Agricultural and Biological Chemistry ◽

10.1080/00021369.1988.10868627 ◽

1988 ◽

Vol 52 (1) ◽

pp. 277-279

Author(s):

Hirokazu Kobayashi

Keyword(s):

Dna Sequencing ◽

Reverse Transcriptase ◽

Dna Fragments ◽

Sequencing Procedure

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Optimization of Polydimethylsiloxane Synthesized Parameters as Vitreous Humour Substitutes

Materials Science Forum ◽

10.4028/www.scientific.net/msf.966.189 ◽

2019 ◽

Vol 966 ◽

pp. 189-193 ◽

Cited By ~ 1

Author(s):

Soni Setiadji ◽

Fitrilawati ◽

Anna Niska Fauza ◽

Atikah Ardi ◽

Ressa Muhrifah Novianti ◽

...

Keyword(s):

Reaction Time ◽

Ring Opening ◽

Vitreoretinal Surgery ◽

Ring Opening Polymerization ◽

Vitreous Humour ◽

Commercial Product ◽

Chain Terminator

Polydimethylsiloxane (PDMS) with two type viscosity that commonly used as vitreous humour substitutes in vitreoretinal surgery has been successfully synthesized by ring-opening polymerization. Optimization of synthesized parameters such as ratio of monomer and chain terminator, the amount of initiator, mixing temperature and reaction time was carried out to obtain PDMS materials having similar properties with commercial product. PDMS with viscosity of 1.17 Pas that very close to viscosity of commercial PDMS of 1.08 Pas, was successfully synthesized with a ratio of 26:10 monomer and terminator chain, 0.58 M of initiator KOH, 170 oC of mixing temperature and 35 minutes of reaction time. By changing reaction time to 40 minutes, PDMS was successfully synthesized with viscosity of 3.42 that similar to that of commercial one.

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