total turnover number

2020 ◽  
Author(s):  
B. Cornils
Keyword(s):  
Turnover Number ◽  
Total Turnover

scholarly journals Supported Tris-Triazole Ligands for Batch and Continuous-Flow Copper-Catalyzed Huisgen 1,3-Dipolar Cycloaddition Reactions

Catalysts ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 434 ◽  
Author(s):  
Alessandra Pucci ◽  
Gianluigi Albano ◽  
Matteo Pollastrini ◽  
Antonio Lucci ◽  
Marialuigia Colalillo ◽  
...  
Keyword(s):  
Cycloaddition Reaction ◽  
Supported Catalyst ◽  
Flow Chemistry ◽  
High Yield ◽  
Dipolar Cycloaddition ◽  
Turnover Number ◽  
Synthetic Sequence ◽  
Total Turnover ◽  
Porous Morphology ◽  
Highly Porous

The lack of supported versions of the tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) ligand, suitable for flow-chemistry applications at scale, prompted us to develop a new route for the immobilization of such tris-triazole chelating units on highly cross-linked polystyrene resins. With this aim, the preparation of the known TBTA-type monomer 3 was optimized to develop a high-yield synthetic sequence, devoid of chromatographic purifications at any stage. Then, bead-type (P7) and monolithic (M7) functional resins were obtained by the easy and scalable suspension- or mold-copolymerization of 3 with divinylbenzene. Both types of materials were found to possess a highly porous morphology and specific surface area in the dry state and could be charged with substantial amounts of Cu(I) or Cu(II) salts. After treatment of the latter with a proper reducing agent, the corresponding supported Cu(I) complexes were tested in the copper-catalyzed alkyne-azide cycloaddition reaction (CuAAC). The immobilized catalysts proved active at room temperature and, in batch and with catalyst loadings as low as 0.6 mol%, afforded quantitative conversions within 20 h. Independent of the alkyne structure, extended use of the supported catalyst in flow was also possible. In the reaction of benzylazide and propargyl alcohol, this allowed a total turnover number larger than 400 to be reached.

scholarly journals E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme

2020 ◽  
Author(s):  
Shiny Joseph Srinivasan ◽  
Sarah Cleary ◽  
Caroline Paul ◽  
Miguel A. Ramirez ◽  
Kylie Vincent
Keyword(s):  
Escherichia Coli ◽  
Turnover Number ◽  
Old Yellow Enzyme ◽  
Nickel Iron Hydrogenase ◽  
Nickel Iron ◽  
E Coli ◽  
Dependent Activity ◽  
Iron Hydrogenase ◽  
Alkene Hydrogenation ◽  
Total Turnover

<p>Robust [NiFe] hydrogenase 1 (Hyd1) from <i>Escherichia coli</i> is shown to have non-native, H<sub>2</sub>-dependent activity for FMN and FAD reduction, and to function as a promising recycling system for FMNH<sub>2</sub> supply to flavoenzymes for chemical synthesis, giving a total turnover number over 10 million when coupled with an Old Yellow Enzyme ene reductase. </p>

Improvement of the total turnover number and space-time yield for chloroperoxidase catalyzed oxidation

1997 ◽  
Vol 55 (2) ◽  
pp. 283-288 ◽  
Author(s):  
K. Seelbach ◽  
M. P. J. van Deurzen ◽  
F. van Rantwijk ◽  
R. A. Sheldon ◽  
U. Kragl
Keyword(s):  
Space Time ◽  
Turnover Number ◽  
Space Time Yield ◽  
Total Turnover ◽  
Catalyzed Oxidation

scholarly journals E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme

2020 ◽  
Author(s):  
Shiny Joseph Srinivasan ◽  
Sarah Cleary ◽  
Caroline Paul ◽  
Miguel A. Ramirez ◽  
Kylie Vincent
Keyword(s):  
Escherichia Coli ◽  
Turnover Number ◽  
Old Yellow Enzyme ◽  
Nickel Iron Hydrogenase ◽  
Nickel Iron ◽  
E Coli ◽  
Dependent Activity ◽  
Iron Hydrogenase ◽  
Alkene Hydrogenation ◽  
Total Turnover

<p>Robust [NiFe] hydrogenase 1 (Hyd1) from <i>Escherichia coli</i> is shown to have non-native, H<sub>2</sub>-dependent activity for FMN and FAD reduction, and to function as a promising recycling system for FMNH<sub>2</sub> supply to flavoenzymes for chemical synthesis, giving a total turnover number over 10 thousand when coupled with an Old Yellow Enzyme ene reductase. </p>

scholarly journals Considerations when Measuring Biocatalyst Performance

Molecules ◽  
2019 ◽  
Vol 24 (19) ◽  
pp. 3573 ◽  
Author(s):  
Mafalda Dias Gomes ◽  
John M. Woodley
Keyword(s):  
Enzyme Stability ◽  
Measurement Techniques ◽  
Accurate Assessment ◽  
Product Concentration ◽  
Turnover Number ◽  
Operating Modes ◽  
Performance Metric ◽  
Total Turnover

As biocatalysis matures, it becomes increasingly important to establish methods with which to measure biocatalyst performance. Such measurements are important to assess immobilization strategies, different operating modes, and reactor configurations, aside from comparing protein engineered variants and benchmarking against economic targets. While conventional measurement techniques focus on a single performance metric (such as the total turnover number), here, it is argued that three metrics (achievable product concentration, productivity, and enzyme stability) are required for an accurate assessment of scalability.

E. coli Nickel-Iron Hydrogenase 1 Catalyses Non-native Reduction of Flavins: Demonstration for Alkene Hydrogenation by Old Yellow Enzyme

2020 ◽  
Author(s):  
Shiny Joseph Srinivasan ◽  
Sarah Cleary ◽  
Caroline Paul ◽  
Miguel A. Ramirez ◽  
Kylie Vincent
Keyword(s):  
Escherichia Coli ◽  
Turnover Number ◽  
Old Yellow Enzyme ◽  
Nickel Iron Hydrogenase ◽  
Nickel Iron ◽  
E Coli ◽  
Dependent Activity ◽  
Iron Hydrogenase ◽  
Alkene Hydrogenation ◽  
Total Turnover

<p>Robust [NiFe] hydrogenase 1 (Hyd1) from <i>Escherichia coli</i> is shown to have non-native, H<sub>2</sub>-dependent activity for FMN and FAD reduction, and to function as a promising recycling system for FMNH<sub>2</sub> supply to flavoenzymes for chemical synthesis, giving a total turnover number over 10 thousand when coupled with an Old Yellow Enzyme ene reductase. </p>

scholarly journals Evaluation of Virtual Water and Water Sustainability of Dairy Production in Trentino Alto Adige (North-Eastern Italy)

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1047
Author(s):  
Pier Paolo Miglietta ◽  
Federica De Leo ◽  
Benedetta Coluccia ◽  
Yari Vecchio ◽  
Fabian Capitanio
Keyword(s):  
Water Availability ◽  
Dairy Products ◽  
Virtual Water ◽  
Resource Depletion ◽  
Small Scale ◽  
Dairy Production ◽  
Water Sustainability ◽  
Freshwater Resource ◽  
Total Turnover ◽  
Protected Designations Of Origin

Dairy products play a significant role in the human diet, but they are often associated with high freshwater resource depletion. In Italy, the dairy sector represents more than 12% of the total turnover of the agri-food sector. Trentino Alto Adige is the first Italian region in terms of number of dairy farms, but it does not register a quantitatively consistent dairy production. Notwithstanding, it is characterized mostly by small-scale farms whose strengths are the Protected Designations of Origin and typical mountain productions. The present study aims at: (i) accounting for the virtual water VW of the main dairy products (milk, butter and cheese) produced in Trentino Alto Adige; (ii) estimating the renewable water resources based on the water flow assessment of the study area; (iii) assessing water sustainability comparing the virtual water consumption of the dairy sector at a regional level to the water availability. The findings show that the consumptive virtual water related to dairy production represents about 1% of the water availability in Trentino Alto Adige. Italy’s domestic dairy production is expanding to meet the growing demand, but the expansion of dairy farming in water-stressed regions should be avoided, preferring instead suitable mountain regions where small-scale farms represent a lively entrepreneurial substrate.

scholarly journals Metalloenzyme mechanisms correlated to their turnover number and metal lability

2021 ◽  
pp. 100004
Author(s):  
Caio B. Castro ◽  
Millena P. Ferreira ◽  
G.C. Marques Netto Caterina
Keyword(s):  
Turnover Number
Sign in / Sign up

Export Citation Format

Share Document