Insights into the In Vitro Formation of Apatite from Mg‐Stabilized Amorphous Calcium Carbonate

2020 ◽  
pp. 2007830
Author(s):  
Phil Opitz ◽  
Laura Besch ◽  
Martin Panthöfer ◽  
Anke Kabelitz ◽  
Ronald E. Unger ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Amorphous Calcium Carbonate

Structural characterization of amorphous calcium carbonate-binding protein: an insight into the mechanism of amorphous calcium carbonate formation

2013 ◽  
Vol 453 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Jingtan Su ◽  
Xiao Liang ◽  
Qiang Zhou ◽  
Guiyou Zhang ◽  
Hongzhong Wang ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Binding Sites ◽  
Binding Protein ◽  
Homology Modelling ◽  
Size Exclusion ◽  
Structural Basis ◽  
Amorphous Calcium Carbonate ◽  
Carbonate Formation

ACC (amorphous calcium carbonate) plays an important role in biomineralization process for its function as a precursor for calcium carbonate biominerals. However, it is unclear how biomacromolecules regulate the formation of ACC precursor in vivo. In the present study, we used biochemical experiments coupled with bioinformatics approaches to explore the mechanisms of ACC formation controlled by ACCBP (ACC-binding protein). Size-exclusion chromatography, chemical cross-linking experiments and negative staining electron microscopy reveal that ACCBP is a decamer composed of two adjacent pentamers. Sequence analyses and fluorescence quenching results indicate that ACCBP contains two Ca2+-binding sites. The results of in vitro crystallization experiments suggest that one Ca2+-binding site is critical for ACC formation and the other site affects the ACC induction efficiency. Homology modelling demonstrates that the Ca2+-binding sites of pentameric ACCBP are arranged in a 5-fold symmetry, which is the structural basis for ACC formation. To the best of our knowledge, this is the first report on the structural basis for protein-induced ACC formation and it will significantly improve our understanding of the amorphous precursor pathway.

In vitro preparation and characterization of amorphous calcium carbonate nanoparticles for applications in curcumin delivery

2019 ◽  
Vol 54 (16) ◽  
pp. 11243-11253 ◽  
Author(s):  
Chaohui Rao ◽  
Xia Guo ◽  
Min Li ◽  
Xiaoqing Sun ◽  
Xiaojie Lian ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Amorphous Calcium Carbonate

Formation of Transient Amorphous Calcium Carbonate Precursor in Quail Eggshell Mineralization:  An In Vitro Study

2006 ◽  
Vol 7 (11) ◽  
pp. 3202-3209 ◽  
Author(s):  
Rajamani Lakshminarayanan ◽  
Xian Jun Loh ◽  
Subramanyam Gayathri ◽  
Swaminathan Sindhu ◽  
Yajnavalka Banerjee ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
In Vitro Study ◽  
Vitro Study ◽  
Amorphous Calcium Carbonate ◽  
Carbonate Precursor

scholarly journals Nano-Hydroxyapatite Derived from Biogenic and Bioinspired Calcium Carbonates: Synthesis and In Vitro Bioactivity

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 264
Author(s):  
Francesca Cestari ◽  
Francesca Agostinacchio ◽  
Anna Galotta ◽  
Giovanni Chemello ◽  
Antonella Motta ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Calcium Carbonate ◽  
Inductively Coupled Plasma ◽  
Calcium Phosphates ◽  
Optical Emission ◽  
Molar Ratio ◽  
In Vitro Tests ◽  
Amorphous Calcium Carbonate ◽  
Calcium Carbonates

Biogenic calcium carbonates naturally contain ions that can be beneficial for bone regeneration and therefore are attractive resources for the production of bioactive calcium phosphates. In the present work, cuttlefish bones, mussel shells, chicken eggshells and bioinspired amorphous calcium carbonate were used to synthesize hydroxyapatite nano-powders which were consolidated into cylindrical pellets by uniaxial pressing and sintering 800–1100 °C. Mineralogical, structural and chemical composition were studied by SEM, XRD, inductively coupled plasma/optical emission spectroscopy (ICP/OES). The results show that the phase composition of the sintered materials depends on the Ca/P molar ratio and on the specific CaCO3 source, very likely associated with the presence of some doping elements like Mg2+ in eggshell and Sr2+ in cuttlebone. Different CaCO3 sources also resulted in variable densification and sintering temperature. Preliminary in vitro tests were carried out (by the LDH assay) and they did not reveal any cytotoxic effects, while good cell adhesion and proliferation was observed at day 1, 3 and 5 after seeding through confocal microscopy. Among the different tested materials, those derived from eggshells and sintered at 900 °C promoted the best cell adhesion pattern, while those from cuttlebone and amorphous calcium carbonate showed round-shaped cells and poorer cell-to-cell interconnection.

In vitro synthesis and stabilization of amorphous calcium carbonate (ACC) nanoparticles within liposomes

CrystEngComm ◽  
2011 ◽  
Vol 13 (12) ◽  
pp. 3975 ◽  
Author(s):  
Chantel C. Tester ◽  
Ryan E. Brock ◽  
Ching-Hsuan Wu ◽  
Minna R. Krejci ◽  
Steven Weigand ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Amorphous Calcium Carbonate ◽  
In Vitro Synthesis

scholarly journals Stabilized Amorphous Calcium Carbonate as a Precursor of Microcoating on Calcite

Materials ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 3762
Author(s):  
Taeyoung Jeon ◽  
Ye-Eun Na ◽  
Dongchan Jang ◽  
Il Won Kim
Keyword(s):  
Calcium Carbonate ◽  
Crystal Morphology ◽  
In Vitro Assay ◽  
Single Crystal Substrate ◽  
Amorphous Calcium Carbonate ◽  
Ethylene Imine ◽  
Vitro Assay ◽  
Crystal Substrate ◽  
Poly Ethylene

Highly controlled biomineralization of calcium carbonate is via non-classical mesocrystallization of amorphous precursors. In the present study, a simple in vitro assay was developed to mimic the biological process, which involved stabilized amorphous calcium carbonate and a single crystal substrate of calcite. The microcoating layer formed on the calcite substrate displayed mesocrystalline characteristics, and the layers near the substrate were strongly influenced by the epitaxy to the substrate. This behavior was preserved even when the morphology of the coating layer was modified with poly(acrylic acid), a model anionic macromolecule. Interestingly, the extent of the epitaxy increased substantially with poly(ethylene imine), which barely affected the crystal morphology. The in vitro assay in the present study will be useful in the investigations of the biomineralization and bioinspired crystallization of calcium carbonate in general.

scholarly journals Gelatinous Siphon Sheath Templates the Starfruit-Shaped Aragonite Aggregate Growth

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Zeng-Qiong Huang ◽  
Gang-Sheng Zhang ◽  
Yuan Tan
Keyword(s):  
Calcium Carbonate ◽  
Growth Rates ◽  
Biomimetic Synthesis ◽  
Surface Adhesion ◽  
Amorphous Calcium Carbonate ◽  
Lattice Matching ◽  
Biomineralization Process ◽  
Aggregate Growth ◽  
Crystallographic Facets

Biomimetic synthesis of aragonite with various templates in vitro is an important way to understand the biomineralization process and synthesize nacre-like materials. Herein, we used the siphon sheath from the bivalve Lutraria sieboldii as the substrate for the formation of calcium carbonate. We found that the inner layer of the sheath, which is composed of approximately 40% protein and 60% β-chitin, induced the formation of nearly pure aragonite by the transformation of amorphous calcium carbonate (ACC). More surprisingly, unique starfruit-shaped aragonite aggregates were observed on the substrate and were constructed from many adhered, oriented aragonite tablets. We consider that the acid-rich protein from the inner layer of the siphon sheath triggers the formation of ACC, and the swollen β-chitin regulates the transformation of ACC into aragonite by lattice matching and stereochemical recognition. The various surface adhesion energies of the crystal, the change in growth rates on different crystallographic facets, and the hexagonal features of the aragonite tablets led to the formation of starfruit-shaped aragonite aggregates.

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