Assembled Cell‐Decorated Collagen (AC‐DC) Fiber Bioprinted Implants with Musculoskeletal Tissue Properties Promote Functional Recovery in Volumetric Muscle Loss

2021 ◽  
pp. 2101357
Author(s):  
Kyle W. Christensen ◽  
Jonathan Turner ◽  
Kelly Coughenour ◽  
Yas Maghdouri‐White ◽  
Anna A. Bulysheva ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Muscle Loss ◽  
Tissue Properties ◽  
Musculoskeletal Tissue ◽  
Volumetric Muscle Loss

scholarly journals Tissue Engineering in Musculoskeletal Tissue: A Review of the Literature

Surgeries ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 58-82
Author(s):  
Mary Bove ◽  
Annalisa Carlucci ◽  
Giovanni Natale ◽  
Chiara Freda ◽  
Antonio Noro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Living Cells ◽  
Physical Factors ◽  
Clinical Use ◽  
Muscle Loss ◽  
Review Of The Literature ◽  
Musculoskeletal Tissue ◽  
Volumetric Muscle Loss ◽  
Structural And Functional Properties ◽  
Near Future

Tissue engineering refers to the attempt to create functional human tissue from cells in a laboratory. This is a field that uses living cells, biocompatible materials, suitable biochemical and physical factors, and their combinations to create tissue-like structures. To date, no tissue engineered skeletal muscle implants have been developed for clinical use, but they may represent a valid alternative for the treatment of volumetric muscle loss in the near future. Herein, we reviewed the literature and showed different techniques to produce synthetic tissues with the same architectural, structural and functional properties as native tissues.

Muscle functional recovery is driven by extracellular vesicles combined with muscle extracellular matrix in a volumetric muscle loss murine model

Biomaterials ◽  
2021 ◽  
pp. 120653
Author(s):  
Fabio Magarotto ◽  
Alberto Sgro ◽  
Agner Henrique Dorigo Hochuli ◽  
Marina Andreetta ◽  
Michele Grassi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Vesicles ◽  
Functional Recovery ◽  
Murine Model ◽  
Muscle Loss ◽  
Volumetric Muscle Loss

scholarly journals Pre-Innervated Tissue Engineered Muscle Promotes a Pro-Regenerative Microenvironment Following Volumetric Muscle Loss

2019 ◽  
Author(s):  
Suradip Das ◽  
Kevin D. Browne ◽  
Franco A. Laimo ◽  
Joseph C. Maggiore ◽  
Halimulati Kaisaier ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Functional Recovery ◽  
The Body ◽  
Muscle Loss ◽  
Skeletal Myocytes ◽  
Volumetric Muscle Loss ◽  
Nanofibrous Scaffolds ◽  
Multiple Challenges

AbstractVolumetric Muscle Loss (VML) is defined as traumatic or surgical loss of skeletal muscle tissue beyond the inherent regenerative capacity of the body, generally leading to a severe functional deficit. Autologous muscle grafts remain the prevalent method of treatment whereas recent muscle repair techniques using biomaterials and tissue engineering are still at a nascent stage and have multiple challenges to address to ensure functional recovery of the injured muscle. Indeed, appropriate somato-motor innervations remain one of the biggest challenges for both autologous muscle grafts as well as tissue engineered muscle constructs. We aim to address this challenge by developing Pre-Innervated Tissue Engineered Muscle comprised of long aligned networks of spinal motor neurons and skeletal myocytes. Here, we developed methodology to biofabricate long fibrils of pre-innervated tissue engineered muscle using a co-culture of myocytes and motor neurons on aligned nanofibrous scaffolds. Motor neurons lead to enhanced differentiation and maturation of skeletal myocytes in vitro. These pre-innervated tissue engineered muscle constructs when implanted in vivo in a rat VML model significantly increase satellite cell migration, micro-vessel formation, and neuromuscular junction density in the host muscle near the injury area at an acute time point as compared to non-pre-innervated myocyte constructs and nanofiber scaffolds alone. These pro-regenerative effects can potentially lead to enhanced functional neuromuscular regeneration following VML, thereby improving the levels of functional recovery following these devastating injuries.

scholarly journals Further Development of a Tissue Engineered Muscle Repair Construct In Vitro for Enhanced Functional Recovery Following Implantation In Vivo in a Murine Model of Volumetric Muscle Loss Injury

2012 ◽  
Vol 18 (11-12) ◽  
pp. 1213-1228 ◽  
Author(s):  
Benjamin T. Corona ◽  
Masood A. Machingal ◽  
Tracy Criswell ◽  
Manasi Vadhavkar ◽  
Ashley C. Dannahower ◽  
...  
Keyword(s):  
Functional Recovery ◽  
Murine Model ◽  
Muscle Loss ◽  
Muscle Repair ◽  
Volumetric Muscle Loss ◽  
Further Development

scholarly journals Tissue Engineering in Musculoskeletal Tissue: Review of Literature

2020 ◽  
Author(s):  
Mary Bove ◽  
Annalisa Carlucci ◽  
Giovanni Natale ◽  
Chiara Freda ◽  
Antonio Noro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Living Cells ◽  
Physical Factors ◽  
Clinical Use ◽  
Muscle Loss ◽  
Review Of Literature ◽  
Musculoskeletal Tissue ◽  
Volumetric Muscle Loss ◽  
Structural And Functional Properties ◽  
Near Future

Tissue engineering, also called “regenerative medicine”, refers to attempt to create functional human tissue from cells in laboratory. This is a field that uses living cells, biocompatible materials, suitable biochemical and physical factors and their combinations, to create tissue-like structures.. To date, no tissue engineered skeletal muscle implants have been developed for clinical use, but it may represent a valid alternative to treat volumetric muscle loss in the near future. Herein, we reviewed the literature and showed different techniques to produce synthetic tissues with the same architectural, structural and functional properties of native tissues.

scholarly journals Assembled Cell-Decorated Collagen (AC-DC) bioprinted implants mimic musculoskeletal tissue properties and promote functional recovery

2021 ◽  
Author(s):  
Kyle W Christensen ◽  
Jonathan Turner ◽  
Kelly Coughenour ◽  
Yas Maghdouri-White ◽  
Anna A Bulysheva ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Functional Recovery ◽  
Cross Sections ◽  
Soft Tissues ◽  
Biomechanical Properties ◽  
Permanent Disability ◽  
Tissue Properties ◽  
Musculoskeletal Tissue ◽  
Strength And Stiffness

Musculoskeletal tissue injuries, including the damage and rupture of ligaments and tendons, and volumetric muscle loss (VML), are exceptionally commonplace and often lead to permanent disability and deformation. We developed an advanced biomanufacturing platform producing cellularized collagen microfiber implants to facilitate functional repair and regeneration of musculoskeletal soft tissues. This Assembled Cell-Decorated Collagen (AC-DC) bioprinting process rapidly and reproducibly forms 3D implants using clinically relevant cells and strong, microfluidic extruded collagen fibers. Quantitative analysis showed that the directionality and distribution of cells throughout AC-DC implants mimic the cellular properties of native musculoskeletal tissue. AC-DC bioprinted implants further approximate or exceed the strength and stiffness of human tendons and ligaments and exceeded the properties of commonplace collagen hydrogels by orders of magnitude. The regenerative potential of AC-DC implants was also assessed in vivo in a rodent VML model. A critically sized muscle injury in the hindlimb was created and repaired, and limb torque generation potential was measured over 12 weeks. Both acellular and cellular implants were found to promote functional recovery compared to the unrepaired group, with AC-DC implants containing therapeutic muscle progenitor cells promoting the highest degree of recovery. Histological analysis and automated image processing of explanted muscle cross-sections revealed increased total muscle fiber count, median muscle fiber size, and increased cellularization for injuries repaired with cellularized implants. These studies introduce the tremendous potential of an advanced bioprinting method for generating tissue analogs with near-native biological and biomechanical properties with the potential to repair numerous challenging musculoskeletal injuries.

Temporal changes in the muscle extracellular matrix due to volumetric muscle loss injury

2021 ◽  
pp. 1-14
Author(s):  
Daniel B. Hoffman ◽  
Christiana J. Raymond-Pope ◽  
Jacob R. Sorensen ◽  
Benjamin T. Corona ◽  
Sarah M. Greising
Keyword(s):  
Extracellular Matrix ◽  
Temporal Changes ◽  
Muscle Loss ◽  
Volumetric Muscle Loss

Evaluation of licofelone as an adjunct anti-inflammatory therapy to biologic scaffolds in the treatment of volumetric muscle loss

2021 ◽  
Author(s):  
Stephen M. Goldman ◽  
Naveena Basa Janakiram ◽  
Michael S. Valerio ◽  
Christopher L. Dearth
Keyword(s):  
Muscle Loss ◽  
Volumetric Muscle Loss ◽  
Anti Inflammatory

scholarly journals Cardiosphere-derived cells, with and without a biological scaffold, stimulate myogenesis and recovery of muscle function in mice with volumetric muscle loss

Biomaterials ◽  
2021 ◽  
Vol 274 ◽  
pp. 120852
Author(s):  
Russell G. Rogers ◽  
Liang Li ◽  
Kiel Peck ◽  
Lizbeth Sanchez ◽  
Weixin Liu ◽  
...  
Keyword(s):  
Muscle Function ◽  
Muscle Loss ◽  
Volumetric Muscle Loss
Sign in / Sign up

Export Citation Format

Share Document