Nanoparticles functionalized with stem cell secretome and CXCR4-overexpressing endothelial membrane for targeted osteoporosis therapy

Background Osteoporosis is a chronic condition affecting patients’ morbidity and mortality and represents a big socioeconomic burden. Because stem cells can proliferate and differentiate into bone-forming cells, stem cell therapy for osteoporosis has been widely studied. However, cells as a live drug face multiple challenges because of their instability during preservation and transportation. In addition, cell therapy has potential adverse effects such as embolism, tumorigenicity, and immunogenicity. Results Herein, we sought to use cell-mimicking and targeted therapeutic nanoparticles to replace stem cells. We fabricated nanoparticles (NPs) using polylactic-co-glycolic acid (PLGA) loaded with the secretome (Sec) from mesenchymal stem cells (MSCs) to form MSC-Sec NPs. Furthermore, we cloaked the nanoparticles with the membranes from C–X–C chemokine receptor type 4 (CXCR4)-expressing human microvascular endothelial cells (HMECs) to generate MSC-Sec/CXCR4 NP. CXCR4 can target the nanoparticles to the bone microenvironment under osteoporosis based on the CXCR4/SDF-1 axis. Conclusions In a rat model of osteoporosis, MSC-Sec/CXCR4 NP were found to accumulate in bone, and such treatment inhibited osteoclast differentiation while promoting osteogenic proliferation. In addition, our results showed that MSC-Sec/CXCR4 NPs reduce OVX-induced bone mass attenuation in OVX rats. Graphical Abstract

Understanding Chinese Gay Men’s and Lesbians’ Experiences of Coping with the Pressure to Marry from the Lens of Suzhi Discourse

Chinese gay men and lesbians are faced with multiple challenges by living in a heteronormative society, and marriage is considered to be a major concern among this group of people. Anchoring from the lens of suzhi discourse, this research carried out a qualitative study by interviewing 21 gay men and lesbians to explore how they refer to the suzhi discourse to justify their choices when faced with the pressure to marry, so as to align themselves more with the mainstream social values. Results indicate that when faced with the pressure to marry, gay men and lesbians refer to the suzhi discourse to construct a positive same-sex identity and civilized community, pursue civilized same-sex relationships, or construct a flexible life.

Challenges of Researchers in Conducting International Study during the Eruption of COVID-19: Student and Mentor Perspectives

Conducting an international research study may bear various challenges; however, during the global COVID-19 crisis, such a study undertakes unpredictable trajectories. This paper explores the challenges experienced by researchers studying Syrian refugees’ physical and mental health and aid workers serving under humanitarian organizations in Lebanon. It includes information about the changes in the study’s goals and design with the emergence and spread of SARS-CoV-2, as necessitated by the circumstances COVID-19 imposed. It focuses on the unique perspectives of the research team of two students and their mentor who faced multiple challenges while involved in the study, and their narratives and subjective experiences that led to new opportunities for growth in the project. The research team specifically engaged in humanistic and existential psychology in order to conduct research in a manner conducive to personal and professional development, productivity and growth. To conclude, the researchers propose recommendations to the academic community on mitigating some of the challenges faced when conducting international research, and suggestions to the humanitarian sector serving vulnerable populations in conflict zones during COVID-19.

Systematic Literature Review: Causes of Rework in GSD

Global Software Development (GSD) involves multiple sites which comprise of different cultures and time zones apart from geographical locations. It is a common software development approach adopted to achieve competitiveness. However, due to multiple challenges it can result in misunderstandings and rework. Rework raises the chance of project failure by delaying the project and increasing the estimated budget. The aim of this study is to identify and categorize the rework causes to reduce its frequency in GSD. To identify the empirical literature related to causes of rework, we performed a Systematic Literature Review (SLR). A total of 23 studies are included as a result of final inclusion. The empirical literature from the year 2009 to 2020 is searched. The overall identified causes of rework in GSD are categorized into 6 major categories which are communication, Requirement Management (RM), roles of stakeholders, product development/integration issues, documentation issues, and differences among stakeholders. The most reported rework causes are related to the category of communication & coordination and RM. Moreover, an industrial survey is conducted to validate the identified rework causes and their mitigation practices from practitioners. This study will help practitioners and researchers in addressing the identified causes and therefore reduce the chances of rework.

Nations Quest for Peace and Alignment to the New Normal: Any Roles for the Marketing Profession?

Peace is one of the most desirable ingredients required for the existence of every human community, and the need for it has quadrupled as a result of the multiple challenges facing the world today. These challenges have further been exacerbated by the need to adjust to the new normal in our contemporary society which nexuses with business operations. The chances for peaceful societies have been fatally sacrificed at the altar of greed, selfish inclinations and the desires for self-aggrandizement of man; and these have made peace a scare commodity. The absence of peace has been linked with catastrophic effects, including business turbulence; which is the reason why deliberate efforts are constantly made by individuals, governments and religious institutions to foster peace. Sadly, the outcomes of these initiatives have not been much to be desired hence the need for a paradigm shift. This paper examined extant literature on the place of the marketing profession in nations’ quest for peace. Peace like every commodity can be marketed; hence the argument of this paper that the marketing mix variables of product, price, place, promotion, preach, propagate and patronize readily find application in marketing peace. This paper concluded on the note that the clamor for a paradigm shift in the quest for peace in nations is in the right direction. More so, such shift must include the involvement of marketing professionals into the army of global peace seekers. Therefore, this paper recommends that national governments should promote matters that foster unity, develop a common national value and deploy social marketing programs. Furthermore, corporate organisations should consider peace marketing as a worthy cause to embrace, and firms should be willing to finance peace building activities as well as form coalitions of mega corporations for the promotion and protection of global peace.

Operation of an alternate care facility in Seoul, Korea: Responding to the third wave of COVID-19 pandemic

The third wave of the COVID-19 outbreak, in December 2020, overwhelmed the Korean healthcare system. In particular, the medical demand of residents in long-term care facilities, homeless shelters, group homes, and detention facilities surged. In this study, we described the experience from development and operation of an alternate care facility (ACF) against the COVID-19 pandemic. In this study, we describe our implementation strategy, including the layout plan, adequate staffing, admission criteria, and patient flow. Using the context of the existing guidelines and literature in the planning and construction process, the differences from the preceding alternative treatment facilities to respond to COVID-19 were also described. Under the time pressure, the old buildings used as office spaces by the US armies were converted into a 107-bed hospital that met infection prevention and clinical care standards. Since these hospitals were dedicated to caring for the patients with COVID-19, multiple challenges were posed to the standards of infection prevention and control, and patient care and safety. The approaches described here provide a potential roadmap for decision makers, who are coping with medical surges as a result of the current and potential future pandemics by implementing an ACF.

Synthesis of Atomically Thin h-BN Layers Using BCl3 and NH3 by Sequential-Pulsed Chemical Vapor Deposition on Cu Foil

The chemical vapor deposition of hexagonal boron nitride layers from BCl3 and NH3 is highly beneficial for scalable synthesis with high controllability, yet multiple challenges such as corrosive reaction or by-product formation have hindered its successful demonstration. Here, we report the synthesis of polycrystalline hexagonal boron nitride (h-BN) layers on copper foil using BCl3 and NH3. The sequential pulse injection of precursors leads to the formation of atomically thin h-BN layers with a polycrystalline structure. The relationship between growth temperature and crystallinity of the h-BN film is investigated using transmission electron microscopy and Raman spectroscopy. Investigation on the initial growth mode achieved by the suppression of precursor supply revealed the formation of triangular domains and existence of preferred crystal orientations. The possible growth mechanism of h-BN in this sequential-pulsed CVD is discussed.

Perceptions of undergraduate medical students regarding e-learning during COVID-19 pandemic

Background: The unprecedented COVID-19 pandemic has caused a sudden shift towards the exclusive adoption of online teaching, forming the primary source of medical education and enabling students to continue to learn remotely. The role in the academic arena has gained importance furthermore considering the ongoing COVID-19 pandemic causing educational institutions around the world over to close down and thus giving rise to multiple challenges at all stages and levels of education in particular for students, due to the lockdown situation. Hence the present study was carried out to assess the perceptions of undergraduate medical students regarding e-learning during COVID-19 pandemic.Methods: A cross-sectional study was carried out from April 2021 to June 2021 among 404 undergraduate medical students of IIMSR Medical College, Badnapur, Jalna, Maharashtra. The questionnaire had eliciting information about socio-demographic profile, knowledge and perception regarding advantages and challenges of e-learning was administered to them. Data was entered in Microsoft excel and analyzed using statistical package for the social sciences (SPSS) version-13.0.Results: About 87% students were of opinion that e-learning was not useful and not motivated to use it and 38% students’ think it’s beneficial as has interactive mode, but 62% students think otherwise. Only 36% are of opinion that e-learning will improve their performance. Majority 78% students think e-learning will not help in better understanding then formal teaching methods.Conclusions: Undergraduate medical students were still more inclined towards traditional teaching rather than e-learning. Faculty members should take necessary measures for improving e-learning facility and quality to help with better learning.

Multiplex, translaminar imaging in the spinal cord of behaving mice

While the spinal cord is known to play critical roles in sensorimotor processing, including pain-related signaling, corresponding activity patterns in genetically defined cell types across spinal laminae have remained elusive. Calcium imaging has enabled cellular activity measurements in behaving rodents but is currently limited to superficial regions. Using chronically implanted microprisms, we imaged sensory and motor evoked activity in regions and at speeds inaccessible by other high-resolution imaging techniques. To enable translaminar imaging in freely behaving animals through implanted microprisms, we additionally developed wearable microscopes with custom-compound microlenses. This new integrated system addresses multiple challenges of previous wearable microscopes, including their limited working distance, resolution, contrast, and achromatic range. The combination of these innovations allowed us to uncover that dorsal horn astrocytes in behaving mice show somatosensory program-dependent and lamina-specific calcium excitation. Additionally, we show that tachykinin precursor 1 (Tac1)-expressing neurons exhibit upper laminae-restricted activity to acute mechanical pain but not locomotion.

Study and Investigation on 5G Technology: A Systematic Review

In wireless communication, Fifth Generation (5G) Technology is a recent generation of mobile networks. In this paper, evaluations in the field of mobile communication technology are presented. In each evolution, multiple challenges were faced that were captured with the help of next-generation mobile networks. Among all the previously existing mobile networks, 5G provides a high-speed internet facility, anytime, anywhere, for everyone. 5G is slightly different due to its novel features such as interconnecting people, controlling devices, objects, and machines. 5G mobile system will bring diverse levels of performance and capability, which will serve as new user experiences and connect new enterprises. Therefore, it is essential to know where the enterprise can utilize the benefits of 5G. In this research article, it was observed that extensive research and analysis unfolds different aspects, namely, millimeter wave (mmWave), massive multiple-input and multiple-output (Massive-MIMO), small cell, mobile edge computing (MEC), beamforming, different antenna technology, etc. This article’s main aim is to highlight some of the most recent enhancements made towards the 5G mobile system and discuss its future research objectives.