Preparation of a cellulose acetate/organic montmorillonite composite porous ultrafine fiber membrane for enzyme immobilization

Cellulose acetate hollow fiber membrane from banana stem fibers coated by TiO2 (CAHFMT) was prepared and characterized as an alternative material for degradation of waste textile dye. Its applicability was demonstrated by mechanical properties, FTIR, SEM, thermal resistance, performance, and degradation efficiency. Cellulose acetate (CA) was synthesized from banana stem fibers by swelling stage, acetylation reaction, and hydrolysis reaction. CA was modified using TiO2 of various concentrations. CAHFMT with 22 % w/v dope concentration has the optimum mechanical properties (stress, strain and Young’s modulus), as well as hydrophilic properties. The performances of CAHFMT with Congo red were determined. The SEM results showed that the membrane had rigid pores. Moreover, this research stated that CAHFMT could be a solution to overcome economical and effective problems.

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Development of Janus Cellulose Acetate Fiber (CA) Membranes for Highly Efficient Oil–Water Separation

Materials ◽

10.3390/ma14205916 ◽

2021 ◽

Vol 14 (20) ◽

pp. 5916

Author(s):

Xiaotian Yu ◽

Xian Zhang ◽

Yajie Xing ◽

Hongjing Zhang ◽

Wuwei Jiang ◽

...

Keyword(s):

Cellulose Acetate ◽

Water Mixture ◽

Permeate Flux ◽

Fiber Membrane ◽

Water Separation ◽

Water Emulsion ◽

X Ray ◽

Centrifugal Spinning ◽

Oil Water Separation ◽

Oil Water

A new type of Janus cellulose acetate (CA) fiber membrane was used to separate oil–water emulsions, which was prepared with plasma gas phase grafting by polymerizing octamethylcyclotetrasiloxane (D4) onto a CA fiber membrane prepared by centrifugal spinning. The Janus–CA fiber membrane was described in terms of chemical structure using Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) analysis, energy dispersive X-ray spectroscopy (EDX) analysis and morphology by field emission scanning electron microscopy (FESEM). In this contribution, we examine the influence of spinning solution concentration, spinning speed and nozzle aperture on the centrifugal spinning process and the fiber morphology. Superhydrophobic/hydrophilic Janus–CA fiber membrane was used to separate water and 1,2-dibromoethane mixture and Toluene-in-water emulsion. Unidirectional water transfer Janus–CA fiber membrane was used to separate n-hexane and water mixture. The separation for the first-time interception rate was about 98.81%, 98.76% and 98.73%, respectively. Experimental results revealed that the Janus cellulose acetate (CA) fiber membrane gave a permeate flux of about 43.32, 331.72 and 275.27 L/(m2·h), respectively. The novel Janus–CA fiber membrane can potentially be used for sustainable W/O emulsion separation. We believe that this is a facile strategy for construction of filtration materials for practical oil–water separation.

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EFFECT OF REACTION CONDITIONS ON THE SYNTHESIS OF CYCLODEXTRIN (CD) BY USING IMMOBILIZED ENZYME

Jurnal Teknologi ◽

10.11113/jt.v80.11707 ◽

2018 ◽

Vol 80 (3) ◽

Author(s):

Suhaily Suhaimi ◽

Rohaida Che Man ◽

Natassha Jamil ◽

Zatul Iffah Mohd Arshad ◽

Shalyda Md Shaarani ◽

...

Keyword(s):

Enzyme Immobilization ◽

Hollow Fiber ◽

Immobilized Enzyme ◽

Hollow Fiber Membrane ◽

Potato Starch ◽

Enzymatic Reaction ◽

Cyclodextrin Glucanotransferase ◽

Fiber Membrane ◽

Reaction Conditions ◽

Agricultural Industries

The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD.

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Formation and use in enzyme immobilization of cellulose acetate-metal alkoxide gels

Carbohydrate Polymers ◽

10.1016/0144-8617(94)90083-3 ◽

1994 ◽

Vol 23 (1) ◽

pp. 1-4 ◽

Cited By ~ 18

Author(s):

Y. Kurokawa ◽

H. Ohta ◽

M. Okubo ◽

M. Takahashi

Keyword(s):

Cellulose Acetate ◽

Enzyme Immobilization ◽

Metal Alkoxide

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Novel PAN/PVP Janus ultrafine fiber membrane and its application for biphasic drug release

Journal of Materials Chemistry B ◽

10.1039/c7tb00929a ◽

2017 ◽

Vol 5 (27) ◽

pp. 5390-5396 ◽

Cited By ~ 40

Author(s):

Yuting Geng ◽

Pan Zhang ◽

Qiutong Wang ◽

Yangxiu Liu ◽

Kai Pan

Keyword(s):

Drug Release ◽

Fiber Membrane ◽

Ultrafine Fiber ◽

Significant Attention

Materials with Janus structures have attracted significant attention in recent years.

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EFFECT OF IMMOBILIZATION PARAMETERS ON THE IMMOBILIZATION OF CYCLODEXTRIN GLUCANOTRANFERASE ON HOLLOW FIBER MEMBRANE

Jurnal Teknologi ◽

10.11113/jt.v82.13881 ◽

2019 ◽

Vol 82 (1) ◽

Author(s):

N. Jamil ◽

R. C. Man ◽

S. Suhaimi ◽

S. M. Shaarani ◽

Z. I. M. Arshad ◽

...

Keyword(s):

Enzyme Immobilization ◽

Hollow Fiber ◽

Contact Time ◽

Hollow Fiber Membrane ◽

Enzymatic Reaction ◽

Chemical Properties ◽

Agitation Rate ◽

Cyclodextrin Glucanotransferase ◽

Fiber Membrane ◽

High Production

Cyclodextrin (CD) is a non-reducing maltooligosaccharides which able to form inclusion complexes with many hydrophobic molecules, changing their physical and chemical properties. With these properties, CD has been discovered to have numerous applications in food industries, pharmaceutical, agricultural and environmental engineering. CD is produced by the enzymatic reaction between cyclodextrin glucanotransferase (CGTase) and starch. Various enzyme immobilization techniques such as adsorption, entrapment, encapsulation and cross-linking have been applied to improve the production of CD. Some of the immobilization parameters such as contact time, agitation rate and pH of the immobilization solution play a vital role in enzyme immobilization process, in order to achieve high production of CD. In the present study, the CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane via adsorption technique. The efficiency of enzyme immobilization appears to be affected by various factors (immobilization parameters) such as contact time, agitation rate and pH. Therefore, the effect of contact time (6-72 h), agitation rate (50-250 rpm) and pH (3-10) on the immobilization of CGTase on PVDF hollow fiber membrane were investigated in this study. The immobilized CGTase exhibited the highest immobilization yield of 69.37% under the conditions of 24 h contact time, 100 rpm and pH 7.0. Therefore, the influence of the immobilization parameters during the enzyme immobilization process is vital in order to achieve the high production of CD. Hence, high immobilization yield contributed to the high production of CD which in turn may be beneficial for the industrial purposes.

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