Enhancement of artemisinin content in tetraploid Artemisia annua plants by modulating the expression of genes in artemisinin biosynthetic pathway

2011 ◽  
Vol 58 (1) ◽  
pp. 50-57 ◽  
Author(s):  
Xiuyan Lin ◽  
Yin Zhou ◽  
Jianjun Zhang ◽  
Xu Lu ◽  
Fangyuan Zhang ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Artemisia Annua ◽  
Expression Of Genes ◽  
Artemisinin Content

Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway

Biologia ◽  
2009 ◽  
Vol 64 (2) ◽  
Author(s):  
Fuyuan Jing ◽  
Ling Zhang ◽  
Meiya Li ◽  
Yueli Tang ◽  
Yuliang Wang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Cell Suspension ◽  
Biosynthetic Pathway ◽  
Gene Expression Analysis ◽  
Artemisia Annua ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Suspension Cultures ◽  
Expression Of Genes ◽  
Artemisinin Content

AbstractArtemisinin, a sesquiterpene lactone endoperoxide derived from Artemisia annua L., is the most effective antimalarial drug. In an effort to increase the artemisinin production, abscisic acid (ABA) with different concentrations (1, 10 and 100 µM) was tested by treating A. annua plants. As a result, the artemisinin content in ABA-treated plants was significantly increased. Especially, artemisinin content in plants treated by 10 µM ABA was 65% higher than that in the control plants, up to an average of 1.84% dry weight. Gene expression analysis showed that in both the ABA-treated plants and cell suspension cultures, HMGR, FPS, CYP71AV1 and CPR, the important genes in the artemisinin biosynthetic pathway, were significantly induced. While only a slight increase of ADS expression was observed in ABA-treated plants, no expression of ADS was detected in cell suspension cultures. This study suggests that there is probably a crosstalk between the ABA signaling pathway and artemisinin biosynthetic pathway and that CYP71AV1, which was induced most significantly, may play a key regulatory role in the artemisinin biosynthetic pathway.

Promotion of artemisinin content in Artemisia annua by overexpression of multiple artemisinin biosynthetic pathway genes

2017 ◽  
Vol 129 (2) ◽  
pp. 251-259 ◽  
Author(s):  
Pu Shi ◽  
Xueqing Fu ◽  
Meng Liu ◽  
Qian Shen ◽  
Weimin Jiang ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Artemisia Annua ◽  
Artemisinin Content

scholarly journals Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Planta ◽  
2021 ◽  
Vol 254 (2) ◽  
Author(s):  
Kaiser Iqbal Wani ◽  
Sadaf Choudhary ◽  
Andleeb Zehra ◽  
M. Naeem ◽  
Pamela Weathers ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Artemisinin Content

scholarly journals Biology of Heme in Mammalian Erythroid Cells and Related Disorders

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Tohru Fujiwara ◽  
Hideo Harigae
Keyword(s):  
Biosynthetic Pathway ◽  
Iron Acquisition ◽  
Protoporphyrin Ix ◽  
Erythroid Differentiation ◽  
Heme Biosynthesis ◽  
Erythroid Cells ◽  
Prosthetic Group ◽  
Expression Of Genes ◽  
Human Disorders ◽  
Pathway Regulation

Heme is a prosthetic group comprising ferrous iron (Fe2+) and protoporphyrin IX and is an essential cofactor in various biological processes such as oxygen transport (hemoglobin) and storage (myoglobin) and electron transfer (respiratory cytochromes) in addition to its role as a structural component of hemoproteins. Heme biosynthesis is induced during erythroid differentiation and is coordinated with the expression of genes involved in globin formation and iron acquisition/transport. However, erythroid and nonerythroid cells exhibit distinct differences in the heme biosynthetic pathway regulation. Defects of heme biosynthesis in developing erythroblasts can have profound medical implications, as represented by sideroblastic anemia. This review will focus on the biology of heme in mammalian erythroid cells, including the heme biosynthetic pathway as well as the regulatory role of heme and human disorders that arise from defective heme synthesis.

scholarly journals Expression Characterization of Flavonoid Biosynthetic Pathway Genes and Transcription Factors in Peanut Under Water Deficit Conditions

2021 ◽  
Vol 12 ◽  
Author(s):  
Ghulam Kubra ◽  
Maryam Khan ◽  
Faiza Munir ◽  
Alvina Gul ◽  
Tariq Shah ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Water Deficit ◽  
Biosynthetic Pathway ◽  
Correlational Analysis ◽  
Accumulation Pattern ◽  
Yield Production ◽  
Expression Of Genes ◽  
Flavonoid Biosynthetic Pathway ◽  
Wide Range

Drought is one of the hostile environmental stresses that limit the yield production of crop plants by modulating their growth and development. Peanut (Arachis hypogaea) has a wide range of adaptations to arid and semi-arid climates, but its yield is prone to loss due to drought. Other than beneficial fatty acids and micronutrients, peanut harbors various bioactive compounds including flavonoids that hold a prominent position as antioxidants in plants and protect them from oxidative stress. In this study, understanding of the biosynthesis of flavonoids in peanut under water deficit conditions was developed through expression analysis and correlational analysis and determining the accumulation pattern of phenols, flavonols, and anthocyanins. Six peanut varieties (BARD479, BARI2011, BARI2000, GOLDEN, PG1102, and PG1265) having variable responses against drought stress have been selected. Higher water retention and flavonoid accumulation have been observed in BARI2011 but downregulation has been observed in the expression of genes and transcription factors (TFs) which indicated the maintenance of normal homeostasis. ANOVA revealed that the expression of flavonoid genes and TFs is highly dependent upon the genotype of peanut in a spatiotemporal manner. Correlation analysis between expression of flavonoid biosynthetic genes and TFs indicated the role of AhMYB111 and AhMYB7 as an inhibitor for AhF3H and AhFLS, respectively, and AhMYB7, AhTTG1, and AhCSU2 as a positive regulator for the expression of Ah4CL, AhCHS, and AhF3H, respectively. However, AhbHLH and AhGL3 revealed nil-to-little relation with the expression of flavonoid biosynthetic pathway genes. Correlational analysis between the expression of TFs related to the biosynthesis of flavonoids and the accumulation of phenolics, flavonols, and anthocyanins indicated coregulation of flavonoid synthesis by TFs under water deficit conditions in peanut. This study would provide insight into the role of flavonoid biosynthetic pathway in drought response in peanut and would aid to develop drought-tolerant varieties of peanut.

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