Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Artemisinin is a sesquiterpene lactone found in Artemisia annua having many medicinal properties. Therefore the factors affecting trichome initiation are important. In the present study, several strategies have been adapted to increase artemisinin production by treating them with nutrients, hormones and elicitors. Abscisic acid, Gibberellic acid (GA3), a combination of GA3+IAA (Gibberellic acid+indole acetic acid), Chitosan, Methyl jasmonate, Acetyl salicylic acid, Lead acetate, sodium chloride, and Nanozime were sprayed on plant at regular intervals. The glandular trichomes of leaves from Artemisia annua (Asteraceae) were examined by light and scanning electron microscopy and the percentage artemisnin content was analyzed by HPTLC. Maximum artemisinin content % and trichome index (0.128) was found when methy jasmonate was applied and minimum artemisinin content % and trichome index was reported in NaCl & acetyl salicylic acid.

Download Full-text

Increase in Artemisia annua Plant Biomass Artemisinin Content and Guaiacol Peroxidase Activity Using the Arbuscular Mycorrhizal Fungus Rhizophagus irregularis

Frontiers in Plant Science ◽

10.3389/fpls.2018.00478 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 9

Author(s):

Erzsébet Domokos ◽

László Jakab-Farkas ◽

Béla Darkó ◽

Béla Bíró-Janka ◽

Gyöngyvér Mara ◽

...

Keyword(s):

Peroxidase Activity ◽

Plant Biomass ◽

Artemisia Annua ◽

Arbuscular Mycorrhizal Fungus ◽

Mycorrhizal Fungus ◽

Arbuscular Mycorrhizal ◽

Rhizophagus Irregularis ◽

Guaiacol Peroxidase ◽

Artemisinin Content

Download Full-text

Correlation among agronomic, physiological and phytochemical traits in Artemisia annua L. varieties

Revista Brasileira de Plantas Medicinais ◽

10.1590/s1516-05722013000100019 ◽

2013 ◽

Vol 15 (1) ◽

pp. 135-141

Author(s):

C.O. Bolina ◽

J.A. Marchese ◽

D. Capelin ◽

D.J. Bertoncelli ◽

C. Pinnow ◽

...

Keyword(s):

Essential Oil ◽

Artemisia Annua ◽

Significant Negative Correlation ◽

Oil Yield ◽

Significance Level ◽

Canopy Volume ◽

The Relationship ◽

Essential Oil Yield ◽

Artemisinin Content ◽

Selection Of

The aim of this study was to estimate the correlations among agronomic, physiological and phytochemical traits of two varieties of Artemisia annua and to identify correlations that can be used in selection and breeding processes for this species. The design was completely randomized and the treatments were for Artemisia annua varieties 2/39x5x3M and 2/39x1V, which were subjected to agronomic, physiological and phytochemical evaluations. The relationship among the studied traits was estimated with Pearson's correlation coefficient, and the significance level of correlations was established by the t-test using Genes software. A significant positive correlation was found between the traits canopy volume and essential oil yield for 2/39x5x3M variety, showing that the selection of plants with a larger canopy volume would facilitate indirect selection of the trait essential oil yield. This correlation was not found for the 2/39x1V variety. In both varieties, there was a significant negative correlation between canopy volume and artemisinin content, which suggested that the selection of plants with a large canopy volume and a large number of branches should be avoided if the goal is to increase artemisinin content.

Download Full-text