Effect of pulsed electromagnetic field on the proliferation and differentiation potential of human bone marrow mesenchymal stem cells

2009 ◽  
Vol 30 (4) ◽  
pp. 251-260 ◽  
Author(s):  
Li-Yi Sun ◽  
Dean-Kuo Hsieh ◽  
Tzai-Chiu Yu ◽  
Hsien-Tai Chiu ◽  
Sheng-Fen Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Electromagnetic Field ◽  
Human Bone ◽  
Pulsed Electromagnetic Field ◽  
Differentiation Potential ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells ◽  
Pulsed Electromagnetic

scholarly journals A Novel Single Pulsed Electromagnetic Field Stimulates Osteogenesis of Bone Marrow Mesenchymal Stem Cells and Bone Repair

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e91581 ◽  
Author(s):  
Yin-Chih Fu ◽  
Chih-Chun Lin ◽  
Je-Ken Chang ◽  
Chung-Hwan Chen ◽  
I-Chun Tai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Electromagnetic Field ◽  
Bone Repair ◽  
Pulsed Electromagnetic Field ◽  
Marrow Mesenchymal Stem Cells ◽  
Pulsed Electromagnetic

scholarly journals The Effect of Culture on Human Bone Marrow Mesenchymal Stem Cells: Focus on DNA Methylation Profiles

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Angela Bentivegna ◽  
Gaia Roversi ◽  
Gabriele Riva ◽  
Laura Paoletta ◽  
Serena Redaelli ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Marrow Mesenchymal Stem Cells

Human bone marrow mesenchymal stem cells (hBM-MSCs) are the best characterized multipotent adult stem cells. Their self-renewal capacity, multilineage differentiation potential, and immunomodulatory properties have indicated that they can be used in many clinical therapies. In a previous work we studied the DNA methylation levels of hBM-MSC genomic DNA in order to delineate a kind of methylation signature specific for early and late passages of culture. In the present work we focused on the modification of the methylation profiles of the X chromosome and imprinted loci, as sites expected to be more stable than whole genome. We propose a model where cultured hBM-MSCs undergo random modifications at the methylation level of most CGIs, nevertheless reflecting the original methylation status. We also pointed out global genome-wide demethylation connected to the long-term culture and senescence. Modification at CGIs promoters of specific genes could be related to the decrease in adipogenic differentiation potential. In conclusion, we showed important changes in CGIs methylation due to long-termin vitroculture that may affect the differentiation potential of hBM-MSCs. Therefore it is necessary to optimize the experimental conditions forin vitroexpansion in order to minimize these epigenetic changes and to standardize safer procedures.

Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells into Motorneuron-like Cells on Electrospun Gelatin Membrane

2014 ◽  
Vol 55 (4) ◽  
pp. 845-853 ◽  
Author(s):  
Faezeh Faghihi ◽  
Esmaeil Mirzaei ◽  
Arash Sarveazad ◽  
Jafar Ai ◽  
Somayeh Ebrahimi Barough ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Differentiation Potential ◽  
Marrow Mesenchymal Stem Cells ◽  
Electrospun Gelatin
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