Regulation of meiosis: From DNA binding protein to protein kinase

BioEssays ◽  
1989 ◽  
Vol 11 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Maureen McLeod
Keyword(s):  
Protein Kinase ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein

scholarly journals Synergistic release from glucose repression by mig1 and ssn mutations in Saccharomyces cerevisiae.

Genetics ◽  
1994 ◽  
Vol 137 (1) ◽  
pp. 49-54 ◽  
Author(s):  
L G Vallier ◽  
M Carlson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Kinase ◽  
Dna Binding ◽  
Glucose Repression ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Yeast Saccharomyces Cerevisiae ◽  
Suc2 Promoter ◽  
Repressor Complex ◽  
Glucose Availability

Abstract In the yeast Saccharomyces cerevisiae, glucose repression of SUC2 transcription requires the SSN6-TUP1 repressor complex. It has been proposed that the DNA-binding protein MIG1 secures SSN6-TUP1 to the SUC2 promoter. Here we show that a mig1 deletion does not cause nearly as dramatic a loss of repression as ssn6: glucose-grown mig1 mutants display 20-fold lower SUC2 expression than ssn6 mutants. Thus, repression by SSN6-TUP1 is not mediated solely by MIG1, but also involves MIG1-independent mechanisms. We report that mig1 partially restores SUC2 expression in mutants lacking the SNF1 protein kinase and show that mig1 is allelic to ssn1, a mutation selected as a suppressor of snf1. Other SSN genes identified in this selection were therefore candidates for a role in repression of SUC2. We show that mig1 acts synergistically with ssn2 through ssn5, ssn7, and ssn8 to relieve glucose repression of SUC2 and to suppress the requirement for SNF1. These findings indicate that the SSN proteins contribute to repression of SUC2, and the pleiotropic phenotypes of the ssn mutants suggest global roles in repression. Finally, the regulated SUC2 expression observed in snf1 mig1 mutants indicates that signals regarding glucose availability can be transmitted independently of the SNF1 protein kinase.

scholarly journals High salt- and SDS-stable DNA binding protein complexes with ATPase and protein kinase activity retained in chromatin-depleted nuclei

1995 ◽  
Vol 23 (8) ◽  
pp. 1359-1366 ◽  
Author(s):  
Benediktas Juodka ◽  
Eberhard Spiess ◽  
Antonella Angiolillo ◽  
Gaby Joswig ◽  
Karsten Rothbarth ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Dna Binding ◽  
Kinase Activity ◽  
Protein Complexes ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
High Salt ◽  
Protein Kinase Activity

Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis.

1991 ◽  
Vol 10 (6) ◽  
pp. 1555-1564 ◽  
Author(s):  
H. Nishitani ◽  
M. Ohtsubo ◽  
K. Yamashita ◽  
H. Iida ◽  
J. Pines ◽  
...  
Keyword(s):  
Dna Replication ◽  
Protein Kinase ◽  
Dna Binding ◽  
Nuclear Dna ◽  
Binding Protein ◽  
Dna Binding Protein

The DNA-binding protein YB-1 is a direct MYCN target and influences repair and resistance in neuroblastoma cell lines

2010 ◽  
Vol 222 (03) ◽  
Author(s):  
S Degen ◽  
S Kuhfittig-Kulle ◽  
JH Schulte ◽  
F Westermann ◽  
A Schramm ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Binding Protein ◽  
Neuroblastoma Cell ◽  
Dna Binding Protein ◽  
Neuroblastoma Cell Lines
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