In situ extraction strategy affects benzophenanthridine alkaloid production fluxes in suspension cultures ofEschscholtzia californica

Macarpine is a minor benzophenanthridine alkaloid with interesting biological activities, which is produced in only a few species of the Papaveraceae family, including Eschscholzia californica. Our present study was focused on the enhancement of macarpine production in E. californica suspension cultures using three elicitation models: salicylic acid (SA) (4; 6; 8 mg/L) elicitation, and simultaneous or sequential combinations of SA and L-tyrosine (1 mmol/L). Sanguinarine production was assessed along with macarpine formation in elicited suspension cultures. Alkaloid production was evaluated after 24, 48 and 72 h of elicitation. Among the tested elicitation models, the SA (4 mg/L), supported by L-tyrosine, stimulated sanguinarine and macarpine production the most efficiently. While sequential treatment led to a peak accumulation of sanguinarine at 24 h and macarpine at 48 h, simultaneous treatment resulted in maximum sanguinarine accumulation at 48 h and macarpine at 72 h. The effect of SA elicitation and precursor supplementation was evaluated also based on the gene expression of 4′-OMT, CYP719A2, and CYP719A3. The gene expression of investigated enzymes was increased at all used elicitation models and their changes correlated with sanguinarine but not macarpine accumulation.

Download Full-text

Stimulation of shikonin production by combined fungal elicitation and in situ extraction in suspension cultures of Arnebia euchroma

Enzyme and Microbial Technology ◽

10.1016/s0141-0229(98)00104-5 ◽

1999 ◽

Vol 24 (5-6) ◽

pp. 243-246 ◽

Cited By ~ 25

Author(s):

Xu-Qing Fu ◽

De-Wei Lu

Keyword(s):

Suspension Cultures ◽

In Situ Extraction ◽

Arnebia Euchroma ◽

Fungal Elicitation ◽

Stimulation Of

Download Full-text

Hexanol biosynthesis from syngas by Clostridium carboxidivorans P7 – product toxicity, temperature dependence and in situ extraction

Heliyon ◽

10.1016/j.heliyon.2021.e07732 ◽

2021 ◽

pp. e07732

Author(s):

Patrick Kottenhahn ◽

Gabriele Philipps ◽

Stefan Jennewein

Keyword(s):

Temperature Dependence ◽

In Situ Extraction ◽

Clostridium Carboxidivorans

Download Full-text

Monoterpenoid Oxindole Alkaloid Production by Uncaria tomentosa (Willd) D.C. Cell Suspension Cultures in a Stirred Tank Bioreactor

Biotechnology Progress ◽

10.1021/bp049608s ◽

2008 ◽

Vol 21 (3) ◽

pp. 786-792 ◽

Cited By ~ 10

Author(s):

Gabriela Trejo-Tapia ◽

Carlos M. Cerda-García-Rojas ◽

Mario Rodríguez-Monroy ◽

Ana C. Ramos-Valdivia

Keyword(s):

Cell Suspension ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Stirred Tank ◽

Alkaloid Production ◽

Stirred Tank Bioreactor ◽

Uncaria Tomentosa

Download Full-text

Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus

Applied and Environmental Microbiology ◽

10.1128/aem.68.4.1524-1533.2002 ◽

2002 ◽

Vol 68 (4) ◽

pp. 1524-1533 ◽

Cited By ~ 3

Author(s):

Florian Helbig ◽

Jörg Steighardt ◽

Werner Roos

Keyword(s):

Uric Acid ◽

Low Molecular Weight ◽

Alkaloid Production ◽

Alkaloid Biosynthesis ◽

Penicillium Cyclopium ◽

Measurable Activity ◽

Reduced Rate ◽

Rate Limiting

ABSTRACT On searching for endogenous, low-molecular-weight effectors of benzodiazepine alkaloid biosynthesis in Penicillium cyclopium uric acid was isolated from ethanolic or autoclaved mycelial extracts of this fungus. The isolation was based on a three-step high-pressure liquid chromatography procedure guided by a microplate bioassay, and uric acid was identified by mass spectrometry and the uricase reaction. Conidiospore suspensions that were treated with this compound during the early phase of outgrowth developed emerged cultures with an enhanced rate of alkaloid production. Uric acid treatment did not increase the in vitro measurable activity of the rate-limiting biosynthetic enzyme, cyclopeptine synthetase. However, these cultures displayed a reduced rate of uptake of the alkaloid precursor l-phenylalanine into the vacuoles of the hyphal cells as assayed in situ. It is suggested that the depressed capacity of vacuolar uptake caused by the contact of outgrowing spores with uric acid liberated from hyphal cells results in an enhanced availability of the precursor l-phenylalanine in the cytoplasm and thus accounts at least in part for the increase in alkaloid production.

Download Full-text