In situ extraction strategy affects benzophenanthridine alkaloid production fluxes in suspension cultures ofEschscholtzia californica

2005 ◽  
Vol 89 (3) ◽  
pp. 280-289 ◽  
Author(s):  
M. Klvana ◽  
R. Legros ◽  
M. Jolicoeur
Keyword(s):  
Suspension Cultures ◽  
Alkaloid Production ◽  
In Situ Extraction ◽  
Benzophenanthridine Alkaloid

scholarly journals Liquid Perfluorodecalin Application for In Situ Extraction and Enhanced Naphthoquinones Production in Arnebia euchroma Cell Suspension Cultures

2014 ◽  
Vol 172 (5) ◽  
pp. 2618-2627 ◽  
Author(s):  
Katarzyna Sykłowska-Baranek ◽  
Maciej Pilarek ◽  
Michał Cichosz ◽  
Agnieszka Pietrosiuk
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
In Situ Extraction ◽  
Arnebia Euchroma

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures

2007 ◽  
Vol 29 (12) ◽  
pp. 2001-2005 ◽  
Author(s):  
Hwa-Young Cho ◽  
Carolyn W. T. Lee-Parsons ◽  
Sung-Yong H. Yoon ◽  
Hong Soon Rhee ◽  
Jong Moon Park
Keyword(s):  
Protein Expression ◽  
Suspension Cultures ◽  
Alkaloid Production ◽  
Eschscholtzia Californica ◽  
Benzophenanthridine Alkaloid

scholarly journals Enhancement of Macarpine Production in Eschscholzia Californica Suspension Cultures under Salicylic Acid Elicitation and Precursor Supplementation

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1261
Author(s):  
Andrea Balažová ◽  
Júlia Urdová ◽  
Vladimír Forman ◽  
Pavel Mučaji
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Biological Activities ◽  
Suspension Cultures ◽  
Sequential Treatment ◽  
Alkaloid Production ◽  
Simultaneous Treatment ◽  
Eschscholzia Californica ◽  
A Minor ◽  
Benzophenanthridine Alkaloid

Macarpine is a minor benzophenanthridine alkaloid with interesting biological activities, which is produced in only a few species of the Papaveraceae family, including Eschscholzia californica. Our present study was focused on the enhancement of macarpine production in E. californica suspension cultures using three elicitation models: salicylic acid (SA) (4; 6; 8 mg/L) elicitation, and simultaneous or sequential combinations of SA and L-tyrosine (1 mmol/L). Sanguinarine production was assessed along with macarpine formation in elicited suspension cultures. Alkaloid production was evaluated after 24, 48 and 72 h of elicitation. Among the tested elicitation models, the SA (4 mg/L), supported by L-tyrosine, stimulated sanguinarine and macarpine production the most efficiently. While sequential treatment led to a peak accumulation of sanguinarine at 24 h and macarpine at 48 h, simultaneous treatment resulted in maximum sanguinarine accumulation at 48 h and macarpine at 72 h. The effect of SA elicitation and precursor supplementation was evaluated also based on the gene expression of 4′-OMT, CYP719A2, and CYP719A3. The gene expression of investigated enzymes was increased at all used elicitation models and their changes correlated with sanguinarine but not macarpine accumulation.

Monoterpenoid Oxindole Alkaloid Production by Uncaria tomentosa (Willd) D.C. Cell Suspension Cultures in a Stirred Tank Bioreactor

2008 ◽  
Vol 21 (3) ◽  
pp. 786-792 ◽  
Author(s):  
Gabriela Trejo-Tapia ◽  
Carlos M. Cerda-García-Rojas ◽  
Mario Rodríguez-Monroy ◽  
Ana C. Ramos-Valdivia
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Stirred Tank ◽  
Alkaloid Production ◽  
Stirred Tank Bioreactor ◽  
Uncaria Tomentosa

scholarly journals Uric Acid Is a Genuine Metabolite of Penicillium cyclopium and Stimulates the Expression of Alkaloid Biosynthesis in This Fungus

2002 ◽  
Vol 68 (4) ◽  
pp. 1524-1533 ◽  
Author(s):  
Florian Helbig ◽  
Jörg Steighardt ◽  
Werner Roos
Keyword(s):  
Uric Acid ◽  
Low Molecular Weight ◽  
Alkaloid Production ◽  
Alkaloid Biosynthesis ◽  
Penicillium Cyclopium ◽  
Measurable Activity ◽  
Reduced Rate ◽  
Rate Limiting

ABSTRACT On searching for endogenous, low-molecular-weight effectors of benzodiazepine alkaloid biosynthesis in Penicillium cyclopium uric acid was isolated from ethanolic or autoclaved mycelial extracts of this fungus. The isolation was based on a three-step high-pressure liquid chromatography procedure guided by a microplate bioassay, and uric acid was identified by mass spectrometry and the uricase reaction. Conidiospore suspensions that were treated with this compound during the early phase of outgrowth developed emerged cultures with an enhanced rate of alkaloid production. Uric acid treatment did not increase the in vitro measurable activity of the rate-limiting biosynthetic enzyme, cyclopeptine synthetase. However, these cultures displayed a reduced rate of uptake of the alkaloid precursor l-phenylalanine into the vacuoles of the hyphal cells as assayed in situ. It is suggested that the depressed capacity of vacuolar uptake caused by the contact of outgrowing spores with uric acid liberated from hyphal cells results in an enhanced availability of the precursor l-phenylalanine in the cytoplasm and thus accounts at least in part for the increase in alkaloid production.

An interchangeable system of hairy root and cell suspension cultures ofCatharanthus roseus for indole alkaloid production

1995 ◽  
Vol 15 (1-2) ◽  
pp. 51-54 ◽  
Author(s):  
Kyung Hee Jung ◽  
Sang Soo Kwak ◽  
Cha Yong Choi ◽  
Jang R. Liu
Keyword(s):  
Cell Suspension ◽  
Hairy Root ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Alkaloid Production

An in-situ extraction–preconcentration method using ionic liquid-based surfactants for the determination of organic contaminants contained in marine sediments

Talanta ◽  
2012 ◽  
Vol 99 ◽  
pp. 972-983 ◽  
Author(s):  
Bárbara Delgado ◽  
Verónica Pino ◽  
Jared L. Anderson ◽  
Juan H. Ayala ◽  
Ana M. Afonso ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Marine Sediments ◽  
Organic Contaminants ◽  
In Situ Extraction ◽  
Preconcentration Method ◽  
Extraction Preconcentration
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