Dynamics of benzophenanthridine alkaloid production in suspension cultures of Eschscholtzia californica after treatment with a yeast elicitor

Macarpine is a minor benzophenanthridine alkaloid with interesting biological activities, which is produced in only a few species of the Papaveraceae family, including Eschscholzia californica. Our present study was focused on the enhancement of macarpine production in E. californica suspension cultures using three elicitation models: salicylic acid (SA) (4; 6; 8 mg/L) elicitation, and simultaneous or sequential combinations of SA and L-tyrosine (1 mmol/L). Sanguinarine production was assessed along with macarpine formation in elicited suspension cultures. Alkaloid production was evaluated after 24, 48 and 72 h of elicitation. Among the tested elicitation models, the SA (4 mg/L), supported by L-tyrosine, stimulated sanguinarine and macarpine production the most efficiently. While sequential treatment led to a peak accumulation of sanguinarine at 24 h and macarpine at 48 h, simultaneous treatment resulted in maximum sanguinarine accumulation at 48 h and macarpine at 72 h. The effect of SA elicitation and precursor supplementation was evaluated also based on the gene expression of 4′-OMT, CYP719A2, and CYP719A3. The gene expression of investigated enzymes was increased at all used elicitation models and their changes correlated with sanguinarine but not macarpine accumulation.

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Monoterpenoid Oxindole Alkaloid Production by Uncaria tomentosa (Willd) D.C. Cell Suspension Cultures in a Stirred Tank Bioreactor

Biotechnology Progress ◽

10.1021/bp049608s ◽

2008 ◽

Vol 21 (3) ◽

pp. 786-792 ◽

Cited By ~ 10

Author(s):

Gabriela Trejo-Tapia ◽

Carlos M. Cerda-García-Rojas ◽

Mario Rodríguez-Monroy ◽

Ana C. Ramos-Valdivia

Keyword(s):

Cell Suspension ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Stirred Tank ◽

Alkaloid Production ◽

Stirred Tank Bioreactor ◽

Uncaria Tomentosa

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An interchangeable system of hairy root and cell suspension cultures ofCatharanthus roseus for indole alkaloid production

Plant Cell Reports ◽

10.1007/bf01690252 ◽

1995 ◽

Vol 15 (1-2) ◽

pp. 51-54 ◽

Cited By ~ 21

Author(s):

Kyung Hee Jung ◽

Sang Soo Kwak ◽

Cha Yong Choi ◽

Jang R. Liu

Keyword(s):

Cell Suspension ◽

Hairy Root ◽

Indole Alkaloid ◽

Cell Suspension Cultures ◽

Suspension Cultures ◽

Alkaloid Production

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Phytoalexin Accumulation in Omithopus sativus as a Response to Elicitor Treatment

Zeitschrift für Naturforschung C ◽

10.1515/znc-1993-7-804 ◽

1993 ◽

Vol 48 (7-8) ◽

pp. 550-555 ◽

Cited By ~ 4

Author(s):

K. Seifert ◽

S. Härtling ◽

A. Porzel ◽

S. Johne

Keyword(s):

Cell Suspension ◽

Cell Suspension Cultures ◽

Spectroscopic Properties ◽

Suspension Cultures ◽

Spore Suspension ◽

Yeast Elicitor ◽

Colletotrichum Trifolii ◽

Intact Plants ◽

Elicitor Treatment ◽

Ornithopus Sativus

An isoflavanoid derivative has been isolated as a phytoalexin from intact plants and cell suspension cultures of Ornithopus sativus after treatment with CuSO4, yeast elicitor, or a spore suspension of Colletotrichum trifolii. Based on its physical and spectroscopic properties the substance was identified as glabridin. After induction, the transient in crease in the content of this phytoalex in was preceded by a transient increase in the activities of PAL and CHS.

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