Dependence on glucose limitation of thepCO2 influences on CHO cell growth, metabolism and IgG production

2007 ◽  
Vol 97 (6) ◽  
pp. 1479-1488 ◽  
Author(s):  
Shinya Takuma ◽  
Chikashi Hirashima ◽  
James M. Piret
Keyword(s):  
Cell Growth ◽  
Cho Cell ◽  
Glucose Limitation ◽  
Growth Metabolism

Effect of surfactant pluronic F-68 on CHO cell growth, metabolism, production, and glycosylation of human recombinant IFN-γ in mild operating conditions

2010 ◽  
Vol 27 (1) ◽  
pp. 181-190 ◽  
Author(s):  
Marie-Françoise Clincke ◽  
Emmanuel Guedon ◽  
Frances T. Yen ◽  
Virginie Ogier ◽  
Olivier Roitel ◽  
...  
Keyword(s):  
Cell Growth ◽  
Operating Conditions ◽  
Cho Cell ◽  
Ifn Γ ◽  
Growth Metabolism

scholarly journals Metabolic excretion associated with nutrient-growth dysregulation promotes the rapid evolution of an overt metabolic defect

2018 ◽  
Author(s):  
Robin Green ◽  
Sonal ◽  
Lin Wang ◽  
Samuel F.M. Hart ◽  
Wenyun Lu ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Growth ◽  
Slow Growth ◽  
Rapid Evolution ◽  
Auxotrophic Mutant ◽  
Ecological Interactions ◽  
Glucose Limitation ◽  
Metabolic Defects ◽  
Fitness Advantage ◽  
Selection Of

AbstractIn eukaryotes, conserved mechanisms ensure that cell growth is coordinated with nutrient availability. Overactive growth during nutrient limitation (“nutrient-growth dysregulation”) can lead to rapid cell death. Here, we demonstrate that cells can adapt to nutrient-growth dysregulation by evolving major metabolic defects. Specifically, when yeast lysine auxotrophic mutant lys- encountered lysine limitation, an evolutionarily novel stress, cells suffered nutrient-growth dysregulation. A sub-population repeatedly evolved to lose the ability to synthesize organosulfurs (lys-orgS-). Organosulfurs, mainly glutathione and glutathione conjugates, were released by lys- cells during lysine limitation when growth was dysregulated, but not during glucose limitation when growth was regulated. Limiting organosulfurs conferred a frequency-dependent fitness advantage to lys-orgS- by eliciting a proper slow growth program including autophagy. Thus, nutrient-growth dysregulation is associated with rapid organosulfur release, which enables the selection of organosulfur auxotrophy to better tune cell growth to the metabolic environment. We speculate that evolutionarily novel stresses can trigger atypical release of certain metabolites, setting the stage for the evolution of new ecological interactions.

scholarly journals Growth Factors Can Influence Cell Growth and Survival through Effects on Glucose Metabolism

2001 ◽  
Vol 21 (17) ◽  
pp. 5899-5912 ◽  
Author(s):  
Matthew G. Vander Heiden ◽  
David R. Plas ◽  
Jeffrey C. Rathmell ◽  
Casey J. Fox ◽  
Marian H. Harris ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Cell Growth ◽  
Glucose Uptake ◽  
Fixed Dose ◽  
Glucose Limitation ◽  
Growth And Survival ◽  
Glucose Levels ◽  
Dependent Cell ◽  
High Concentrations

ABSTRACT Cells from multicellular organisms are dependent upon exogenous signals for survival, growth, and proliferation. The relationship among these three processes was examined using an interleukin-3 (IL-3)-dependent cell line. No fixed dose of IL-3 determined the threshold below which cells underwent apoptosis. Instead, increasing growth factor concentrations resulted in progressive shortening of the G1 phase of the cell cycle and more rapid proliferative expansion. Increased growth factor concentrations also resulted in proportional increases in glycolytic rates. Paradoxically, cells growing in high concentrations of growth factor had an increased susceptibility to cell death upon growth factor withdrawal. This susceptibility correlated with the magnitude of the change in the glycolytic rate following growth factor withdrawal. To investigate whether changes in the availability of glycolytic products influence mitochondrion-initiated apoptosis, we artificially limited glycolysis by manipulating the glucose levels in the medium. Like growth factor withdrawal, glucose limitation resulted in Bax translocation, a decrease in mitochondrial membrane potential, and cytochromec redistribution to the cytosol. In contrast, increasing cell autonomous glucose uptake by overexpression of Glut1 significantly delayed apoptosis following growth factor withdrawal. These data suggest that a primary function of growth factors is to regulate glucose uptake and metabolism and thus maintain mitochondrial homeostasis and enable anabolic pathways required for cell growth. Consistent with this hypothesis, expression of the three genes involved in glucose uptake and glycolytic commitment, those for Glut1, hexokinase 2, and phosphofructokinase 1, was found to rapidly decline to nearly undetectable levels following growth factor withdrawal.

scholarly journals Engineering CHO cell growth by stable manipulation of miRNA expression

2011 ◽  
Vol 5 (S8) ◽  
Author(s):  
Noëlia Sanchez ◽  
Nga Lao ◽  
Clair Gallagher ◽  
Martin Clynes ◽  
Niall Barron
Keyword(s):  
Cell Growth ◽  
Mirna Expression ◽  
Cho Cell

Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsulein vivo

2007 ◽  
Vol 84B (1) ◽  
pp. 79-88 ◽  
Author(s):  
Ying Zhang ◽  
Wei Wang ◽  
Yubing Xie ◽  
Weiting Yu ◽  
Guojun Lv ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cho Cell

Effects of dissolved oxygen on hybridoma cell growth, metabolism, and antibody production kinetics in continuous culture

1990 ◽  
Vol 6 (6) ◽  
pp. 437-446 ◽  
Author(s):  
Sadettin S. Ozturk ◽  
Bernhard O. Palsson
Keyword(s):  
Cell Growth ◽  
Dissolved Oxygen ◽  
Continuous Culture ◽  
Antibody Production ◽  
Production Kinetics ◽  
Growth Metabolism

scholarly journals Yeast cells under glucose-limitation environment need increased response cost for osmostress defense

2021 ◽  
Author(s):  
Chunxiong Luo ◽  
Wenting Shen ◽  
Ziqing Gao ◽  
Kaiyue Chen ◽  
Qi Ouyang
Keyword(s):  
Cell Growth ◽  
Environmental Changes ◽  
Yeast Cells ◽  
Protein Accumulation ◽  
Response Cost ◽  
Glucose Limitation ◽  
Dynamic Expression ◽  
Normal Glucose ◽  
Low Glucose ◽  
Glucose Condition

Cells always make responses to environmental changes, involving dynamic expression of tens to hundreds of proteins. This response system may demand substantial cost and thus affect cell growth. Here, we studied the cell's responses to osmostress under glucose-limitation environments. Through analyzed thirteen osmotic-downstream proteins and two related transcription factors, we found that the cells required stronger responses under low glucose concentrations than normal glucose condition after being stimulated by osmostress, even the cell growth rate was unchanged in these two constant conditions. We proposed and verified that under a glucose-limitation environment, the glycolysis intermediates were limited (defense reserve saving), which caused that cells needed more glycerol production enzymes to adapt to the osmostress. Further experiments proved that this 'defense reserve-saving' strategy required cells to spend more response cost when facing stress, which on the other hand, enhanced the fitness for the coming environment variations via protein accumulation reserve.

The Influence of pH on Cell Growth and Specific Productivity of Two CHO Cell Lines Producing Human Anti Rh D IgG

2001 ◽  
pp. 197-199 ◽  
Author(s):  
Maria J. De Jesus ◽  
M. Bourgeois ◽  
G. Baumgartner ◽  
P. Tromba ◽  
Martin Jordan ◽  
...  
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Specific Productivity ◽  
Cho Cell ◽  
Influence Of Ph

Influence of the rapeseed protein hydrolysis process on CHO cell growth

2008 ◽  
Vol 99 (15) ◽  
pp. 7143-7151 ◽  
Author(s):  
G. Chabanon ◽  
L. Alves da Costa ◽  
B. Farges ◽  
C. Harscoat ◽  
S. Chenu ◽  
...  
Keyword(s):  
Cell Growth ◽  
Protein Hydrolysis ◽  
Cho Cell ◽  
Rapeseed Protein ◽  
Hydrolysis Process
Sign in / Sign up

Export Citation Format

Share Document