scholarly journals Comparative lipid production by oleaginous yeasts in hydrolyzates of lignocellulosic biomass and process strategy for high titers

2016 ◽  
Vol 113 (8) ◽  
pp. 1676-1690 ◽  
Author(s):  
Patricia J. Slininger ◽  
Bruce S. Dien ◽  
Cletus P. Kurtzman ◽  
Bryan R. Moser ◽  
Erica L. Bakota ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Lipid Production ◽  
Oleaginous Yeasts ◽  
Process Strategy

scholarly journals High‐yield lipid production from lignocellulosic biomass using engineered xylose‐utilizing Yarrowia lipolytica

GCB Bioenergy ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 670-679 ◽  
Author(s):  
Sang Do Yook ◽  
Jiwon Kim ◽  
Gyeongtack Gong ◽  
Ja Kyong Ko ◽  
Youngsoon Um ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
High Yield

scholarly journals Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia

2018 ◽  
Vol 19 (4) ◽  
pp. 1266-1272 ◽  
Author(s):  
MICKY VINCENT ◽  
HUANG CHAI HUNG ◽  
PATRICIA ROWENA MARK BARAN ◽  
AFIZUL SAFWAN AZAHARI ◽  
DAYANG SALWANI AWANG ADENI
Keyword(s):  
Candida Parapsilosis ◽  
Yeast Species ◽  
Lipid Production ◽  
Industrial Applications ◽  
Oleaginous Yeasts ◽  
Intracellular Lipids ◽  
Pichia Manshurica ◽  
Sudan Iv ◽  
Scientific Technical ◽  
Polymerase Chain

Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeastsfrom Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine thediversity of oleaginous yeasts from various sources in Kuching, Sarawak (Malaysia). Microscopic observations via light and scanningelectron microscope (SEM) indicated that the yeast isolates were in sizes ranging from 2-3 μm in width and 4-8 μm in length, typical ofmost unicellular ascomycotic fungi. Polymerase Chain Reaction (PCR) and molecular identification performed on the yeast isolates,targeting the D1/D2 region of the 26S rDNA, identified 6 yeast species from the 21 isolates, namely Pichia manshurica (5/21), Candidakrusei (8/21), Candida parapsilosis (1/21), Pichia guilliermondii (2/21), Clavispora lusitaniae (1/21) and Kluyveromyces marxianus(4/21). All 21 yeast isolates accumulated intracellular lipids when grown in nitrogen-limited medium, as tested via Sudan IV staining.The present study is the first to document the production of lipids bodies in C. krusei, C. parapsilosis, and C. lusitaniae. Furtherinvestigations to assess the growth kinetics, lipid production efficiencies and lipids profiles of these oleaginous yeasts may provideinsights into the possible utilization of these isolates for a variety of scientific, technical and industrial applications.

scholarly journals Engineering the Oleaginous Yeast Rhodosporidium toruloides for Improved Resistance Against Inhibitors in Biomass Hydrolysates

2021 ◽  
Vol 9 ◽  
Author(s):  
Liting Lyu ◽  
Yadong Chu ◽  
Sufang Zhang ◽  
Yue Zhang ◽  
Qitian Huang ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cell Mass ◽  
Peroxidase Gene ◽  
The Past ◽  
Yeast Strains ◽  
Biomass Hydrolysates ◽  
Improved Performance ◽  
Inhibitor Resistance

Conversion of lignocellulosic biomass into lipids and related chemicals has attracted much attention in the past two decades, and the oleaginous yeast Rhodosporidiumtoruloides has been widely used in this area. While R. toruloides species naturally have physiological advantages in terms of substrate utilization, lipid accumulation, and inhibitor resistance, reduced lipid production and cell growth are noticed when biomass hydrolysates are used as feedstocks. To improve the robustness of R. toruloides, here, we devised engineered strains by overexpressing genes responsible for phenolic compound degradation. Specifically, gene expression cassettes of the manganese peroxidase gene (MNP) and versatile peroxidase gene (VP) were constructed and integrated into the genome of R. toruloides NP11. A series of engineered strains were evaluated for lipid production in the presence of typical phenolic inhibitors. The results showed that R. toruloides strains with proper expression of MNP or VP indeed grew faster in the presence of vanillin and 5-hydroxymethylfurfural than the parental strain. When cultivated in concentrated mode biomass hydrolysates, the strain VP18 had improved performance as the cell mass and lipid content increased by 30% and 25%, respectively. This study provides more robust oleaginous yeast strains for microbial lipid production from lignocellulosic biomass, and similar efforts may be used to devise more advanced lipid producers.

Engineering the filamentous fungusNeurospora crassafor lipid production from lignocellulosic biomass

2014 ◽  
Vol 111 (6) ◽  
pp. 1097-1107 ◽  
Author(s):  
Christine M. Roche ◽  
N. Louise Glass ◽  
Harvey W. Blanch ◽  
Douglas S. Clark
Keyword(s):  
Lignocellulosic Biomass ◽  
Lipid Production

Microbial lipid production from pectin-derived carbohydrates by oleaginous yeasts

2015 ◽  
Vol 50 (7) ◽  
pp. 1097-1102 ◽  
Author(s):  
Yandan Wang ◽  
Zhiwei Gong ◽  
Xiaobing Yang ◽  
Hongwei Shen ◽  
Qian Wang ◽  
...  
Keyword(s):  
Lipid Production ◽  
Microbial Lipid ◽  
Oleaginous Yeasts ◽  
Microbial Lipid Production

Recycling microbial lipid production wastes to cultivate oleaginous yeasts

2015 ◽  
Vol 175 ◽  
pp. 91-96 ◽  
Author(s):  
Xiaobing Yang ◽  
Guojie Jin ◽  
Zhiwei Gong ◽  
Hongwei Shen ◽  
Fengwu Bai ◽  
...  
Keyword(s):  
Lipid Production ◽  
Microbial Lipid ◽  
Oleaginous Yeasts ◽  
Production Wastes ◽  
Microbial Lipid Production
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