Engineering the filamentous fungusNeurospora crassafor lipid production from lignocellulosic biomass

2014 ◽  
Vol 111 (6) ◽  
pp. 1097-1107 ◽  
Author(s):  
Christine M. Roche ◽  
N. Louise Glass ◽  
Harvey W. Blanch ◽  
Douglas S. Clark
Keyword(s):  
Lignocellulosic Biomass ◽  
Lipid Production

scholarly journals High‐yield lipid production from lignocellulosic biomass using engineered xylose‐utilizing Yarrowia lipolytica

GCB Bioenergy ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 670-679 ◽  
Author(s):  
Sang Do Yook ◽  
Jiwon Kim ◽  
Gyeongtack Gong ◽  
Ja Kyong Ko ◽  
Youngsoon Um ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
High Yield

scholarly journals Engineering the Oleaginous Yeast Rhodosporidium toruloides for Improved Resistance Against Inhibitors in Biomass Hydrolysates

2021 ◽  
Vol 9 ◽  
Author(s):  
Liting Lyu ◽  
Yadong Chu ◽  
Sufang Zhang ◽  
Yue Zhang ◽  
Qitian Huang ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cell Mass ◽  
Peroxidase Gene ◽  
The Past ◽  
Yeast Strains ◽  
Biomass Hydrolysates ◽  
Improved Performance ◽  
Inhibitor Resistance

Conversion of lignocellulosic biomass into lipids and related chemicals has attracted much attention in the past two decades, and the oleaginous yeast Rhodosporidiumtoruloides has been widely used in this area. While R. toruloides species naturally have physiological advantages in terms of substrate utilization, lipid accumulation, and inhibitor resistance, reduced lipid production and cell growth are noticed when biomass hydrolysates are used as feedstocks. To improve the robustness of R. toruloides, here, we devised engineered strains by overexpressing genes responsible for phenolic compound degradation. Specifically, gene expression cassettes of the manganese peroxidase gene (MNP) and versatile peroxidase gene (VP) were constructed and integrated into the genome of R. toruloides NP11. A series of engineered strains were evaluated for lipid production in the presence of typical phenolic inhibitors. The results showed that R. toruloides strains with proper expression of MNP or VP indeed grew faster in the presence of vanillin and 5-hydroxymethylfurfural than the parental strain. When cultivated in concentrated mode biomass hydrolysates, the strain VP18 had improved performance as the cell mass and lipid content increased by 30% and 25%, respectively. This study provides more robust oleaginous yeast strains for microbial lipid production from lignocellulosic biomass, and similar efforts may be used to devise more advanced lipid producers.

scholarly journals Techno-Economic Analysis of Biodiesel Production from Microbial Oil Using Cardoon Stalks as Carbon Source

Energies ◽  
2021 ◽  
Vol 14 (5) ◽  
pp. 1473
Author(s):  
Marco Castellini ◽  
Stefano Ubertini ◽  
Diego Barletta ◽  
Ilaria Baffo ◽  
Pietro Buzzini ◽  
...  
Keyword(s):  
Economic Analysis ◽  
Lignocellulosic Biomass ◽  
Production Cost ◽  
Lipid Production ◽  
Lipid Extraction ◽  
Production Costs ◽  
Raw Material ◽  
Biodiesel Production ◽  
Microbial Oil ◽  
Lipid Yield

Today one of the most interesting ways to produce biodiesel is based on the use of oleaginous microorganisms, which can accumulate microbial oil with a composition similar to vegetable oils. In this paper, we present a thermo-chemical numerical model of the yeast biodiesel production process, considering cardoon stalks as raw material. The simulation is performed subdividing the process into the following sections: steam explosion pre-treatment, enzymatic hydrolysis, lipid production, lipid extraction, and alkali-catalyzed transesterification. Numerical results show that 406.4 t of biodiesel can be produced starting from 10,000 t of lignocellulosic biomass. An economic analysis indicates a biodiesel production cost of 12.8 USD/kg, thus suggesting the need to increase the capacity plant and the lipid yield to make the project economically attractive. In this regard, a sensitivity analysis is also performed considering an ideal lipid yield of 22% and 100,000 t of lignocellulosic biomass. The biodiesel production costs related to these new scenarios are 7.88 and 5.91 USD/kg, respectively. The large capacity plant combined with a great lipid yield in the fermentation stage shows a biodiesel production cost of 3.63 USD/kg making the product competitive on the current market of biofuels by microbial oil.

scholarly journals Strategies for Improvement of Lipid Production by Yeast Trichosporon oleaginosus from Lignocellulosic Biomass

2021 ◽  
Vol 7 (11) ◽  
pp. 934
Author(s):  
Marina Grubišić ◽  
Katarina Mihajlovski ◽  
Ana Marija Gruičić ◽  
Sunčica Beluhan ◽  
Božidar Santek ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Low Cost ◽  
Lipid Production ◽  
Tween 80 ◽  
Biodiesel Production ◽  
Hydrolysis Rate ◽  
Efficient Production ◽  
Fed Batch ◽  
Batch Mode ◽  
Lipid Yield

Microbial lipids have similar fatty acid composition to plant oils, and therefore, are considered as an alternative feedstock for biodiesel production. Oleaginous yeasts accumulate considerable amounts of lipids intracellularly during growth on low-cost renewable feedstocks such as lignocellulosic biomass. In this study, we cultivated yeast Trichosporon oleaginosus on hydrolysate of alkaline pretreated corn cobs. Different process configurations were evaluated and compared, including separate hydrolysis and fermentation (SHF) with cellulase recycle and simultaneous saccharification and fermentation (SSF) in batch and fed-batch mode. At low enzyme loading, the highest lipid concentration of 26.74 g L−1 was reached in fed-batch SSF fed with 2.5% (g g−1) substrate. Batch SHF was conducted for four rounds with recycling the cellulase adsorbed on unhydrolyzed lignocellulosic biomass. Thirty percent of cellulase saving was achieved for rounds 2–4 without compromising productivity and lipid yield. The addition of Tween 80 to lignocellulosic slurry improved the hydrolysis rate of structural carbohydrates in pretreated lignocellulosic biomass. Furthermore, supplementing the growth medium with Tween 80 improved lipid yield and productivity without affecting yeast growth. Oleaginous yeast T. oleaginosus is a promising strain for the sustainable and efficient production of lipids from renewable lignocellulosic feedstock.

Lignocellulosic biomass as a carbohydrate source for lipid production by Mortierella isabellina

2013 ◽  
Vol 128 ◽  
pp. 385-391 ◽  
Author(s):  
Jijiao Zeng ◽  
Yubin Zheng ◽  
Xiaochen Yu ◽  
Liang Yu ◽  
Difeng Gao ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Lipid Production ◽  
Carbohydrate Source ◽  
Mortierella Isabellina

A survey of yeast from the Yarrowia clade for lipid production in dilute acid pretreated lignocellulosic biomass hydrolysate

2016 ◽  
Vol 101 (8) ◽  
pp. 3319-3334 ◽  
Author(s):  
Josh Quarterman ◽  
Patricia J. Slininger ◽  
Cletus P. Kurtzman ◽  
Stephanie R. Thompson ◽  
Bruce S. Dien
Keyword(s):  
Lignocellulosic Biomass ◽  
Lipid Production ◽  
Dilute Acid ◽  
Biomass Hydrolysate

scholarly journals Comparative lipid production by oleaginous yeasts in hydrolyzates of lignocellulosic biomass and process strategy for high titers

2016 ◽  
Vol 113 (8) ◽  
pp. 1676-1690 ◽  
Author(s):  
Patricia J. Slininger ◽  
Bruce S. Dien ◽  
Cletus P. Kurtzman ◽  
Bryan R. Moser ◽  
Erica L. Bakota ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Lipid Production ◽  
Oleaginous Yeasts ◽  
Process Strategy
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