scholarly journals High‐yield lipid production from lignocellulosic biomass using engineered xylose‐utilizing Yarrowia lipolytica

GCB Bioenergy ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 670-679 ◽  
Author(s):  
Sang Do Yook ◽  
Jiwon Kim ◽  
Gyeongtack Gong ◽  
Ja Kyong Ko ◽  
Youngsoon Um ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
High Yield

Direct conversion of cellulose to high-yield methyl lactate over Ga-doped Zn/H-nanozeolite Y catalysts in supercritical methanol

2017 ◽  
Vol 19 (8) ◽  
pp. 1969-1982 ◽  
Author(s):  
Deepak Verma ◽  
Rizki Insyani ◽  
Young-Woong Suh ◽  
Seung Min Kim ◽  
Seok Ki Kim ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Value Added ◽  
Direct Conversion ◽  
High Yield ◽  
Supercritical Methanol ◽  
Methyl Lactate ◽  
High Yields

For realizing sustainable bio-based refineries, it is crucial to obtain high yields of value-added chemicalsviadirect conversion of cellulose and lignocellulosic biomass.

Increased Lipid Production in Yarrowia lipolytica from Acetate through Metabolic Engineering and Cosubstrate Fermentation

2021 ◽  
Author(s):  
Lin Chen ◽  
Wei Yan ◽  
Xiujuan Qian ◽  
Minjiao Chen ◽  
Xiaoyu Zhang ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Yarrowia Lipolytica ◽  
Lipid Production

scholarly journals pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose

2019 ◽  
Vol 290 ◽  
pp. 10-15 ◽  
Author(s):  
Shuyan Zhang ◽  
Sujit Sadashiv Jagtap ◽  
Anshu Deewan ◽  
Christopher V. Rao
Keyword(s):  
Citric Acid ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Limited Growth

scholarly journals Engineering the Oleaginous Yeast Rhodosporidium toruloides for Improved Resistance Against Inhibitors in Biomass Hydrolysates

2021 ◽  
Vol 9 ◽  
Author(s):  
Liting Lyu ◽  
Yadong Chu ◽  
Sufang Zhang ◽  
Yue Zhang ◽  
Qitian Huang ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cell Mass ◽  
Peroxidase Gene ◽  
The Past ◽  
Yeast Strains ◽  
Biomass Hydrolysates ◽  
Improved Performance ◽  
Inhibitor Resistance

Conversion of lignocellulosic biomass into lipids and related chemicals has attracted much attention in the past two decades, and the oleaginous yeast Rhodosporidiumtoruloides has been widely used in this area. While R. toruloides species naturally have physiological advantages in terms of substrate utilization, lipid accumulation, and inhibitor resistance, reduced lipid production and cell growth are noticed when biomass hydrolysates are used as feedstocks. To improve the robustness of R. toruloides, here, we devised engineered strains by overexpressing genes responsible for phenolic compound degradation. Specifically, gene expression cassettes of the manganese peroxidase gene (MNP) and versatile peroxidase gene (VP) were constructed and integrated into the genome of R. toruloides NP11. A series of engineered strains were evaluated for lipid production in the presence of typical phenolic inhibitors. The results showed that R. toruloides strains with proper expression of MNP or VP indeed grew faster in the presence of vanillin and 5-hydroxymethylfurfural than the parental strain. When cultivated in concentrated mode biomass hydrolysates, the strain VP18 had improved performance as the cell mass and lipid content increased by 30% and 25%, respectively. This study provides more robust oleaginous yeast strains for microbial lipid production from lignocellulosic biomass, and similar efforts may be used to devise more advanced lipid producers.

Harnessing the Effect of pH on Lipid Production in Batch Cultures of Yarrowia lipolytica SKY7

2017 ◽  
Vol 184 (4) ◽  
pp. 1332-1346 ◽  
Author(s):  
Mathiazhakan Kuttiraja ◽  
Ayed Dhouha ◽  
Rajeshwar Dayal Tyagi
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Effect Of Ph ◽  
Batch Cultures

scholarly journals Enhanced lipid production by Yarrowia lipolytica cultured with synthetic and waste-derived high-content volatile fatty acids under alkaline conditions

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Ruiling Gao ◽  
Zifu Li ◽  
Xiaoqin Zhou ◽  
Wenjun Bao ◽  
Shikun Cheng ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Yarrowia Lipolytica ◽  
Butyric Acid ◽  
Lipid Content ◽  
Volatile Fatty Acids ◽  
Lipid Production ◽  
Initial Ph ◽  
Alkaline Conditions

Abstract Background Volatile fatty acids (VFAs) can be effective and promising alternate carbon sources for microbial lipid production by a few oleaginous yeasts. However, the severe inhibitory effect of high-content (> 10 g/L) VFAs on these yeasts has impeded the production of high lipid yields and their large-scale application. Slightly acidic conditions have been commonly adopted because they have been considered favorable to oleaginous yeast cultivation. However, the acidic pH environment further aggravates this inhibition because VFAs appear largely in an undissociated form under this condition. Alkaline conditions likely alleviate the severe inhibition of high-content VFAs by significantly increasing the dissociation degree of VFAs. This hypothesis should be verified through a systematic research. Results The combined effects of high acetic acid concentrations and alkaline conditions on VFA utilization, cell growth, and lipid accumulation of Yarrowia lipolytica were systematically investigated through batch cultures of Y. lipolytica by using high concentrations (30–110 g/L) of acetic acid as a carbon source at an initial pH ranging from 6 to 10. An initial pH of 8 was determined as optimal. The highest biomass and lipid production (37.14 and 10.11 g/L) were obtained with 70 g/L acetic acid, whereas cultures with > 70 g/L acetic acid had decreased biomass and lipid yield due to excessive anion accumulation. Feasibilities on high-content propionic acid, butyric acid, and mixed VFAs were compared and evaluated. Results indicated that YX/S and YL/S of cultures on butyric acid (0.570, 0.144) were comparable with those on acetic acid (0.578, 0.160) under alkaline conditions. The performance on propionic acid was much inferior to that on other acids. Mixed VFAs were more beneficial to fast adaptation and lipid production than single types of VFA. Furthermore, cultures on food waste (FW) and fruit and vegetable waste (FVW) fermentate were carried out and lipid production was effectively improved under this alkaline condition. The highest biomass and lipid production on FW fermentate reached 14.65 g/L (YX/S: 0.414) and 3.20 g/L (YL/S: 0.091) with a lipid content of 21.86%, respectively. By comparison, the highest biomass and lipid production on FVW fermentate were 11.84 g/L (YX/S: 0.534) and 3.08 g/L (YL/S: 0.139), respectively, with a lipid content of 26.02%. Conclusions This study assumed and verified that alkaline conditions (optimal pH 8) could effectively alleviate the lethal effect of high-content VFA on Y. lipolytica and significantly improve biomass and lipid production. These results could provide a new cultivation strategy to achieve simple utilizations of high-content VFAs and increase lipid production. Feasibilities on FW and FVW-derived VFAs were evaluated, and meaningful information was provided for practical applications.

scholarly journals High-throughput fermentation screening for the yeast Yarrowia lipolytica with real-time monitoring of biomass and lipid production

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Alexandre Back ◽  
Tristan Rossignol ◽  
François Krier ◽  
Jean-Marc Nicaud ◽  
Pascal Dhulster
Keyword(s):  
Real Time ◽  
Yarrowia Lipolytica ◽  
High Throughput ◽  
Lipid Production ◽  
Real Time Monitoring ◽  
Yeast Yarrowia Lipolytica

Engineering the filamentous fungusNeurospora crassafor lipid production from lignocellulosic biomass

2014 ◽  
Vol 111 (6) ◽  
pp. 1097-1107 ◽  
Author(s):  
Christine M. Roche ◽  
N. Louise Glass ◽  
Harvey W. Blanch ◽  
Douglas S. Clark
Keyword(s):  
Lignocellulosic Biomass ◽  
Lipid Production
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