Experimental determination of flux control distribution in biochemical systems: In vitro model to analyze transient metabolite concentrations

Information about the elimination and the adequate dosing of levofloxacin during renal replacement therapy is scarce. The aim of this study was to characterize in vitro the elimination of levofloxacin during continuous venovenous hemodialysis (CVVHD) and to investigate whether the CVVHD clearances of creatinine and urea are correlated with the levofloxacin clearance in order to facilitate dosage adjustments. An in vitro model of CVVHD was established using five dialyzer membranes at varying dialysate flow rates applied in the clinical setting (8, 16, 25, 33 and 41 ml/min). Plasma and dialysate samples were drawn for determination of levofloxacin, creatinine and urea concentrations to evaluate clearances by CVVHD. During CVVHD, the clearance of levofloxacin varied between 9.02 and 33.30 ml/min, depending on the chosen setup. Positive correlations (p<0.001) were received for: dialysate flow rate (QD) and creatinine/urea clearances (R>0.93); QD and levofloxacin clearance (R 0.59–0.71); levofloxacin and creatinine clearance (R 0.69–0.75); and levofloxacin and urea clearance (R 0.56–0.75) as well. When dosing critically ill patients, therefore, extracorporeal as well as total clearance of levofloxacin should be considered.

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Comparison of Two Different In Vivo Models and an In Vitro Model for Caloric Determination of Four Novel Fiber Ingredients

Journal of Agricultural and Food Chemistry ◽

10.1021/jf402385s ◽

2013 ◽

Vol 61 (50) ◽

pp. 12374-12379 ◽

Cited By ~ 3

Author(s):

Sarah Cervantes-Pahm ◽

Brenda K. Knapp ◽

Beob G. Kim ◽

Yanhong Liu ◽

Carl M. Parsons ◽

...

Keyword(s):

In Vitro Model ◽

In Vivo Models ◽

Vitro Model

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Determination of the pharmacodynamic activity of clinically achievable tigecycline serum concentrations against clinical isolates of Escherichia coli with extended-spectrum -lactamases, AmpC -lactamases and reduced susceptibility to carbapenems using an in vitro model

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/dkp254 ◽

2009 ◽

Vol 64 (4) ◽

pp. 824-828 ◽

Cited By ~ 3

Author(s):

G. G. Zhanel ◽

P. J. Baudry ◽

F. Tailor ◽

L. Cox ◽

D. J. Hoban ◽

...

Keyword(s):

Escherichia Coli ◽

In Vitro Model ◽

Clinical Isolates ◽

Serum Concentrations ◽

Extended Spectrum ◽

Vitro Model

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Volume-rendered, three-dimensional echocardiographi determination of the size, shape, and position of atrial septal defects: Validation in an in vitro model

American Heart Journal ◽

10.1016/s0002-8703(96)90436-6 ◽

1996 ◽

Vol 132 (2) ◽

pp. 376-381 ◽

Cited By ~ 27

Author(s):

Giuseppina Magni ◽

Qi-Ling Cao ◽

Lissa Sugeng ◽

Alain Delabays ◽

Gerald Marx ◽

...

Keyword(s):

In Vitro Model ◽

Three Dimensional ◽

Septal Defects ◽

Atrial Septal Defects ◽

Vitro Model

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In vivo determination of surface tension in the horse trachea and in vitro model studies

Respiration Physiology ◽

10.1016/s0034-5687(97)84032-7 ◽

1997 ◽

Vol 109 (1) ◽

pp. 81-93 ◽

Cited By ~ 51

Author(s):

V Im Hof ◽

P Gehr ◽

V Gerber ◽

M.M Lee ◽

S Schürch

Keyword(s):

Surface Tension ◽

In Vitro Model ◽

Model Studies ◽

Vitro Model

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Effect of vanadium(IV) on the growth of Artemisia tilesii “hairy” root culture

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v25.1177 ◽

2019 ◽

Vol 25 ◽

pp. 277-280

Author(s):

N. A. Matvieieva ◽

O. A. Gavryliuk ◽

V. P. Duplij

Keyword(s):

Hairy Root ◽

In Vitro Model ◽

Root Culture ◽

Hairy Root Culture ◽

Transgenic Roots ◽

Vitro Model ◽

Mass Increase ◽

Plant Shoots

Aim. Determination of the effect of toxic metals on plants is of considerable interest, since contamination of the environment with toxicants is a threat to the safety of agriculture and human health. Vanadium increased content in soil leads to the suppression of plant growth. The aim of the work was to determine the peculiarities of the influence of V(IV) (neutral solution [V4+ cit]) on Artemisia tilesii Ledeb plants in the in vitro model system and to compare this effect on the growth of “hairy” root culture and plants. Methods. Plant shoots and transgenic roots were cultivated on a solidified Murashige and Skoog medium with V(IV) at 1, 5, 10, 50 and 100 mg/L. Mass increase was measured after 4 weeks. Results. It was found that V(IV) at 50 mg/L concentration completely inhibited root formation and led to shoot death. Transgenic roots survived even at 100 mg/L of vanadium. Conclusions. The results of the work demonstrated that A. tilesii “hairy” roots were characterized by greater resistance to V(IV) than control plants. In addition, differences in the sensitivity of different root lines to this compound were detected. This fact probably could be explained by the transformation, the transfer of agrobacterial genes, and changes in the secondary metabolism in transgenic roots. Keywords: vanadium(IV), Artemisia tilesii Ledeb., “hairy” root culture.

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