Metabolic engineering of a robust Escherichia coli strain with a dual protection system

2019 ◽  
Vol 116 (12) ◽  
pp. 3333-3348 ◽  
Author(s):  
Xiao‐Yang Ou ◽  
Xiao‐Ling Wu ◽  
Fei Peng ◽  
Ying‐Jie Zeng ◽  
Hui‐Xian Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Coli Strain ◽  
Protection System ◽  
Dual Protection

Designing an Escherichia coli Strain for Phenylalanine Overproduction by Metabolic Engineering

2017 ◽  
Vol 59 (4-5) ◽  
pp. 168-178 ◽  
Author(s):  
Neetu Tyagi ◽  
Deepti Saini ◽  
Richa Guleria ◽  
Krishna Jyoti Mukherjee
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Coli Strain

scholarly journals Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system

2010 ◽  
Vol 9 (1) ◽  
pp. 21 ◽  
Author(s):  
Adelfo Escalante ◽  
Rocío Calderón ◽  
Araceli Valdivia ◽  
Ramón de Anda ◽  
Georgina Hernández ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Shikimic Acid ◽  
Coli Strain ◽  
Phosphotransferase System

scholarly journals Enhancement of β-Alanine Biosynthesis in Escherichia coli Based on Multivariate Modular Metabolic Engineering

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1017
Author(s):  
Jian Xu ◽  
Li Zhou ◽  
Zhemin Zhou
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Industrial Production ◽  
Metabolic Flux ◽  
Coli Strain ◽  
Biosynthesis Pathway ◽  
Fed Batch ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Fed Batch Fermentation

β-alanine is widely used as an intermediate in industrial production. However, the low production of microbial cell factories limits its further application. Here, to improve the biosynthesis production of β-alanine in Escherichia coli, multivariate modular metabolic engineering was recruited to manipulate the β-alanine biosynthesis pathway through keeping the balance of metabolic flux among the whole metabolic network. The β-alanine biosynthesis pathway was separated into three modules: the β-alanine biosynthesis module, TCA module, and glycolysis module. Global regulation was performed throughout the entire β-alanine biosynthesis pathway rationally and systematically by optimizing metabolic flux, overcoming metabolic bottlenecks and weakening branch pathways. As a result, metabolic flux was channeled in the direction of β-alanine biosynthesis without huge metabolic burden, and 37.9 g/L β-alanine was generated by engineered Escherichia coli strain B0016-07 in fed-batch fermentation. This study was meaningful to the synthetic biology of β-alanine industrial production.

Metabolic Engineering of Escherichia coli for Lacto-N-triose II Production with High Productivity

2021 ◽  
Author(s):  
Yingying Zhu ◽  
Li Wan ◽  
Jiawei Meng ◽  
Guocong Luo ◽  
Geng Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
High Productivity

scholarly journals The Role of Dipeptidases in Cells of Escherichia coli Strain K-12

1966 ◽  
Vol 241 (11) ◽  
pp. 2502-2508 ◽  
Author(s):  
Sofia Simmonds
Keyword(s):  
Escherichia Coli ◽  
Coli Strain ◽  
K 12 ◽  
In Cells

scholarly journals Regulation of Expression of the TIR-Containing Protein C Gene of the Uropathogenic Escherichia coli Strain CFT073

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 549
Author(s):  
Julia Ittensohn ◽  
Jacqueline Hemberger ◽  
Hannah Griffiths ◽  
Maren Keller ◽  
Simone Albrecht ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein C ◽  
Interleukin 1 ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Reporter Construct ◽  
Regulation Of Expression ◽  
E Coli ◽  
Strain Cft073 ◽  
Myeloid Differentiation Factor

The uropathogenic Escherichia coli strain CFT073 causes kidney abscesses in mice Toll/interleukin-1 receptor domain-containing protein C (TcpC) dependently and the corresponding gene is present in around 40% of E. coli isolates of pyelonephritis patients. It impairs the Toll-like receptor (TLR) signaling chain and the NACHT leucin-rich repeat PYD protein 3 inflammasome (NLRP3) by binding to TLR4 and myeloid differentiation factor 88 as well as to NLRP3 and caspase-1, respectively. Overexpression of the tcpC gene stopped replication of CFT073. Overexpression of several tcpC-truncation constructs revealed a transmembrane region, while its TIR domain induced filamentous bacteria. Based on these observations, we hypothesized that tcpC expression is presumably tightly controlled. We tested two putative promoters designated P1 and P2 located at 5′ of the gene c2397 and 5′ of the tcpC gene (c2398), respectively, which may form an operon. High pH and increasing glucose concentrations stimulated a P2 reporter construct that was considerably stronger than a P1 reporter construct, while increasing FeSO4 concentrations suppressed their activity. Human urine activated P2, demonstrating that tcpC might be induced in the urinary tract of infected patients. We conclude that P2, consisting of a 240 bp region 5′ of the tcpC gene, represents the major regulator of tcpC expression.

Sign in / Sign up

Export Citation Format

Share Document