scholarly journals Regulation of Expression of the TIR-Containing Protein C Gene of the Uropathogenic Escherichia coli Strain CFT073

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 549
Author(s):  
Julia Ittensohn ◽  
Jacqueline Hemberger ◽  
Hannah Griffiths ◽  
Maren Keller ◽  
Simone Albrecht ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein C ◽  
Interleukin 1 ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Reporter Construct ◽  
Regulation Of Expression ◽  
E Coli ◽  
Strain Cft073 ◽  
Myeloid Differentiation Factor

The uropathogenic Escherichia coli strain CFT073 causes kidney abscesses in mice Toll/interleukin-1 receptor domain-containing protein C (TcpC) dependently and the corresponding gene is present in around 40% of E. coli isolates of pyelonephritis patients. It impairs the Toll-like receptor (TLR) signaling chain and the NACHT leucin-rich repeat PYD protein 3 inflammasome (NLRP3) by binding to TLR4 and myeloid differentiation factor 88 as well as to NLRP3 and caspase-1, respectively. Overexpression of the tcpC gene stopped replication of CFT073. Overexpression of several tcpC-truncation constructs revealed a transmembrane region, while its TIR domain induced filamentous bacteria. Based on these observations, we hypothesized that tcpC expression is presumably tightly controlled. We tested two putative promoters designated P1 and P2 located at 5′ of the gene c2397 and 5′ of the tcpC gene (c2398), respectively, which may form an operon. High pH and increasing glucose concentrations stimulated a P2 reporter construct that was considerably stronger than a P1 reporter construct, while increasing FeSO4 concentrations suppressed their activity. Human urine activated P2, demonstrating that tcpC might be induced in the urinary tract of infected patients. We conclude that P2, consisting of a 240 bp region 5′ of the tcpC gene, represents the major regulator of tcpC expression.

scholarly journals Uropathogenic Escherichia coli strain CFT073 disrupts NLRP3 inflammasome activation

2016 ◽  
Vol 126 (7) ◽  
pp. 2425-2436 ◽  
Author(s):  
Anna Waldhuber ◽  
Manoj Puthia ◽  
Andreas Wieser ◽  
Christine Cirl ◽  
Susanne Dürr ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Nlrp3 Inflammasome ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Inflammasome Activation ◽  
Nlrp3 Inflammasome Activation ◽  
Strain Cft073

scholarly journals Pectic Oligosaccharides from Cranberry Prevent Quiescence and Persistence in the Uropathogenic Escherichia coli CFT073

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jiadong Sun ◽  
Robert W. Deering ◽  
Zhiyuan Peng ◽  
Laila Najia ◽  
Christina Khoo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Chemical Compounds ◽  
Uropathogenic Escherichia Coli ◽  
Cranberry Juice ◽  
Persister Cells ◽  
E Coli ◽  
Pectic Oligosaccharides ◽  
Bladder Cells ◽  
Strain Cft073 ◽  
Tract Infections

AbstractUrinary tract infections (UTIs) caused by Escherichia coli create a large burden on healthcare and frequently lead to recurrent infections. Part of the success of E. coli as an uropathogenic bacterium can be attributed to its ability to form quiescent intracellular reservoirs in bladder cells and its persistence after antibiotic treatment. Cranberry juice and related products have been used for the prevention of UTIs with varying degrees of success. In this study, a group of cranberry pectic oligosaccharides (cPOS) were found to both inhibit quiescence and reduce the population of persister cells formed by the uropathogenic strain, CFT073. This is the first report detailing constituents of cranberry with the ability to modulate these important physiological aspects of uropathogenic E. coli. Further studies investigating cranberry should be keen to include oligosaccharides as part of the ‘active’ cocktail of chemical compounds.

scholarly journals Regulation of Type 1 Fimbriae by Unlinked FimB- and FimE-Like Recombinases in Uropathogenic Escherichia coli Strain CFT073

2006 ◽  
Vol 74 (2) ◽  
pp. 1072-1083 ◽  
Author(s):  
Andrew Bryan ◽  
Paula Roesch ◽  
Lindsay Davis ◽  
Rebecca Moritz ◽  
Shahaireen Pellett ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Similarity ◽  
Epidemiological Survey ◽  
Coli Strain ◽  
Amino Acid Sequences ◽  
Reciprocal Relationship ◽  
E Coli ◽  
Normal Human ◽  
Strain Cft073

ABSTRACT Genomic DNA sequence analysis of the uropathogenic Escherichia coli strain CFT073 revealed that besides the fimB and fimE recombinase genes that control the type 1 pilus fim phase switch, there are three additional fimB- and fimE-like genes: ipuA, ipuB, and ipbA. Alignment of the predicted amino acid sequences showed that the five recombinases range in sequence similarity from 63 to 70%. An epidemiological survey indicates that ipuA and ipuB are present and linked next to the dsdCXA locus in 24 of 67 uropathogenic E. coli strains but are found in only 1 of 15 normal human fecal isolates. The ipbA sequence located next to the betABIT locus was found in 42 of 67 uropathogenic isolates and 8 of 15 of the commensal strains. We show that two of these recombinases, those encoded by ipuA and ipbA, can function at the type 1 pilus fim switch. In a CFT073 deletion mutant lacking all five recombinase genes, recombinant ipuA or ipbA provided in trans inverted the fim element from the off state to the on state. When a fim OFF CFT073 ΔfimBE mutant was used to infect the urinary tracts of mice, a switch to the fim on state was detected within 24 h in bacteria recovered from urine, the bladder, and the kidneys. A fim OFF CFT073 ΔfimBE ipuB ipbA mutant also demonstrated the ability to switch from the fim off state to the on state during mouse infection. CFT073 recombinase mutants derived from isolates in either the fim on or off state showed a reciprocal relationship for motility. Switches from a nonmotile to a motile phenotype and from a fim on to off genotype were observed in fim ON CFT073 ΔfimBE ipuAB ipbA mutants when ipuA or fimB was provided in trans. Together these results indicate that ipuA has fimB-like on-to-off and off-to-on fim switching activity and that ipbA has the ability to switch fim from the off to the on orientation.

The Pix pilus adhesin of the uropathogenic Escherichia coli strain X2194 (O2 : K− : H6) is related to Pap pili but exhibits a truncated regulatory region

Microbiology ◽  
2003 ◽  
Vol 149 (6) ◽  
pp. 1387-1397 ◽  
Author(s):  
Andreas Lügering ◽  
Inga Benz ◽  
Sabine Knochenhauer ◽  
Michael Ruffing ◽  
M. Alexander Schmidt
Keyword(s):  
Escherichia Coli ◽  
Regulatory Region ◽  
Coli Strain ◽  
Open Reading Frames ◽  
Transcriptional Level ◽  
E Coli ◽  
Strain Cft073 ◽  
State Growth ◽  
Chelatobacter Heintzii ◽  
Tract Infections

Adhesins provide a major advantage for uropathogenic Escherichia coli in establishing urinary tract infections (UTIs). A novel gene cluster responsible for the expression of a filamentous adhesin of the pyelonephritogenic E. coli strain X2194 has been identified, molecularly cloned, and characterized. The ‘pix operon’ contains eight open reading frames which exhibit significant sequence homology to corresponding genes in the pap operon encoding P pili, the prevalent E. coli adhesins in non-obstructive acute pyelonephritis in humans. Although a pixB gene corresponding to the PapB regulator was identified, a papI homologue could not be found in the pix operon. Instead, a fragment of the R6 gene of the highly uropathogenic E. coli strain CFT073 was identified upstream of pixB. The R6 gene is located in a pathogenicity island containing several pilus-encoding sequences and shows homology to a transposase of Chelatobacter heintzii. In a pixA–lacZ fusion system it was demonstrated that the expression of Pix pili is regulated at the transcriptional level by the R6 gene sequence. A significantly reduced transcription was observed by deleting this fragment and by lowering the growth temperature from 37 to 26 °C. In contrast to other filamentous adhesin systems, Pix pili are mainly expressed in the steady state growth phase and were not repressed by the addition of glucose.

scholarly journals Analysis of the Virulence of Uropathogenic Escherichia coli Strain CFT073 in the Murine Urinary Tract

BIO-PROTOCOL ◽  
2017 ◽  
Vol 7 (3) ◽  
Author(s):  
Anna Waldhuber ◽  
Manoj Puthia ◽  
Andreas Wieser ◽  
Catharina Svanborg ◽  
Thomas Miethke
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Strain Cft073

scholarly journals Draft Whole-Genome Sequence of a Uropathogenic Escherichia coli Strain Carrying the eae Gene

2019 ◽  
Vol 8 (43) ◽  
Author(s):  
Tiago Barcelos Valiatti ◽  
Fernanda Fernandes Santos ◽  
Ana Carolina de Mello Santos ◽  
Rosa Maria Silva ◽  
Eneas Carvalho ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Whole Genome Sequence ◽  
Intestinal Cells ◽  
Whole Genome ◽  
Content Type ◽  
E Coli ◽  
Tract Infections

Uropathogenic Escherichia coli (UPEC) strains are responsible for most cases of urinary tract infections worldwide. We present the draft whole-genome sequence of the UPEC 252 strain, which carries the eae gene that encodes the intimin adhesin. Intimin promotes intimate adherence of enteropathogenic E. coli and enterohemorrhagic E. coli to intestinal cells.

scholarly journals Draft Genome Sequence of Uropathogenic Escherichia coli Strain NB8

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Xing-bei Weng ◽  
Zu-huang Mi ◽  
Chun-xin Wang ◽  
Jian-ming Zhu
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Genome Sequence ◽  
Draft Genome ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Draft Genome Sequence ◽  
Beta Lactams ◽  
E Coli ◽  
Genes Encoding

Escherichia coli NB8 is a clinical pyelonephritis isolate. Here, we report the draft genome sequence of uropathogenic E. coli NB8, which contains drug resistance genes encoding resistance to beta-lactams, aminoglycosides, quinolones, macrolides, colistin, sulfonamide-trimethoprim, and tetracycline. NB8 infects the kidney and bladder, making it an important tool for studying E. coli pathogenesis.

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