Flux redistribution of central carbon metabolism for efficient production of L‐tryptophan in Escherichia coli

2021 ◽  
Author(s):  
Bo Xiong ◽  
Yongduo Zhu ◽  
Daoguang Tian ◽  
Shuai Jiang ◽  
Xiaoguang Fan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Efficient Production ◽  
Central Carbon ◽  
Flux Redistribution

Genetic manipulation of Escherichia coli central carbon metabolism for efficient production of fumaric acid

2018 ◽  
Vol 270 ◽  
pp. 96-102 ◽  
Author(s):  
Huan Liu ◽  
Ruirui Song ◽  
Yue Liang ◽  
Ting Zhang ◽  
Li Deng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fumaric Acid ◽  
Carbon Metabolism ◽  
Genetic Manipulation ◽  
Central Carbon Metabolism ◽  
Efficient Production ◽  
Central Carbon

Improvement of hydrogen production by metabolic engineering of Escherichia coli: Modification on both the PTS system and central carbon metabolism

2020 ◽  
Vol 45 (9) ◽  
pp. 5687-5696 ◽  
Author(s):  
Victor E. Balderas-Hernandez ◽  
Kathya P. Landeros Maldonado ◽  
Arturo Sánchez ◽  
Adam Smoliński ◽  
Antonio De Leon Rodriguez
Keyword(s):  
Escherichia Coli ◽  
Metabolic Engineering ◽  
Hydrogen Production ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Central Carbon

Modeling the interaction between the central carbon metabolism of Escherichia coli and bioreactor culture media

2020 ◽  
Vol 163 ◽  
pp. 107753
Author(s):  
Fabián A. Ortega-Quintana ◽  
Mauricio A. Trujillo-Roldán ◽  
Héctor Botero-Castro ◽  
Hernan Alvarez
Keyword(s):  
Escherichia Coli ◽  
Carbon Metabolism ◽  
Culture Media ◽  
Central Carbon Metabolism ◽  
Bioreactor Culture ◽  
Central Carbon

scholarly journals The Role of Metabolites in the Link between DNA Replication and Central Carbon Metabolism in Escherichia coli

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 447
Author(s):  
Klaudyna Krause ◽  
Monika Maciąg-Dorszyńska ◽  
Anna Wosinski ◽  
Lidia Gaffke ◽  
Joanna Morcinek-Orłowska ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Dna Replication ◽  
Protein Interactions ◽  
Carbon Metabolism ◽  
Molecular Mechanisms ◽  
Central Carbon Metabolism ◽  
Replication Initiation ◽  
Cell Physiology ◽  
E Coli ◽  
Central Carbon

A direct link between DNA replication regulation and central carbon metabolism (CCM) has been previously demonstrated in Bacillus subtilis and Escherichia coli, as effects of certain mutations in genes coding for replication proteins could be specifically suppressed by particular mutations in genes encoding CCM enzymes. However, specific molecular mechanism(s) of this link remained unknown. In this report, we demonstrate that various CCM metabolites can suppress the effects of mutations in different replication genes of E. coli on bacterial growth, cell morphology, and nucleoid localization. This provides evidence that the CCM-replication link is mediated by metabolites rather than direct protein-protein interactions. On the other hand, action of metabolites on DNA replication appears indirect rather than based on direct influence on the replication machinery, as rate of DNA synthesis could not be corrected by metabolites in short-term experiments. This corroborates the recent discovery that in B. subtilis, there are multiple links connecting CCM to DNA replication initiation and elongation. Therefore, one may suggest that although different in detail, the molecular mechanisms of CCM-dependent regulation of DNA replication are similar in E. coli and B. subtilis, making this regulation an important and common constituent of the control of cell physiology in bacteria.

scholarly journals Development of an accurate kinetic model for the central carbon metabolism of Escherichia coli

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Nusrat Jahan ◽  
Kazuhiro Maeda ◽  
Yu Matsuoka ◽  
Yurie Sugimoto ◽  
Hiroyuki Kurata
Keyword(s):  
Escherichia Coli ◽  
Kinetic Model ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Central Carbon

scholarly journals Emergence of growth and dormancy from a kinetic model of the Escherichia coli central carbon metabolism

2022 ◽  
Author(s):  
Yusuke Himeoka ◽  
Namiko Mitarai
Keyword(s):  
Escherichia Coli ◽  
Kinetic Model ◽  
Carbon Metabolism ◽  
Wide Spectrum ◽  
Central Carbon Metabolism ◽  
Bacterial Cells ◽  
Futile Cycle ◽  
Dormant State ◽  
Dormant Cells ◽  
Central Carbon

Abstract Physiological states of bacterial cells exhibit a wide spectrum of timescale. Under nutrient-rich conditions, most of the cells in an isogenic bacterial population grow at certain rates, while a small subpopulation sometimes stays in a dormant state where the growth rates slow down by orders of magnitude. For revealing the origins of such heterogeneity of timescales, we studied the kinetic model of Escherichia coli central carbon metabolism including the dynamics of the energy currency molecules. We found that the model robustly exhibits both the growing- and the dormant state. In order to unveil the mechanism of distinct behaviours, we developed a recursive method to simplify the model without changing the qualitative feature of the dynamics. Analytical and numerical studies of the 2-variable minimal model revealed the necessary conditions for the distinct behaviour, namely, the depletion of energy due to the futile cycle and its non-uniform impact on the kinetics because of the coexistence of the energy currency-coupled and uncoupled reactions as well as branching of the network. The result is consistent with the experimental reports that the dormant cells commonly exhibit low ATP levels, and provides a possible explanation for the appearance of dormant cells that causes antibiotic persistence.

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