Crosslinking of Streptavidin–Biotinylated Bovine Serum Albumin Studied with Fluorescence Correlation Spectroscopy

2020 ◽  
Author(s):  
Sujin Lee ◽  
Hahkjoon Kim
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Correlation Spectroscopy ◽  
Bovine Serum ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

Tracking structural transitions of bovine serum albumin in surfactant solutions by fluorescence correlation spectroscopy and fluorescence lifetime analysis

Soft Matter ◽  
2015 ◽  
Vol 11 (12) ◽  
pp. 2512-2518 ◽  
Author(s):  
Xuzhu Zhang ◽  
Andrzej Poniewierski ◽  
Sen Hou ◽  
Krzysztof Sozański ◽  
Agnieszka Wisniewska ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Fluorescence Lifetime ◽  
Fluorescence Correlation Spectroscopy ◽  
Bovine Serum ◽  
Structural Changes ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Surfactant Solutions ◽  
Lifetime Analysis

Sudden structural changes of BSA in surfactant solutions are observed from FCS curves.

scholarly journals The application of two-dimensional fluorescence correlation spectroscopy on the interaction between bovine serum albumin and prulifloxacin

2009 ◽  
Vol 23 (5-6) ◽  
pp. 257-263 ◽  
Author(s):  
Mao-Yun Xue ◽  
Ai-Ping Yang ◽  
Mei-Hua Ma ◽  
Xiao-Hua Li
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fluorescence Spectra ◽  
Resolution Enhancement ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Fluorescence Correlation ◽  
Enthalpy Changes ◽  
2D Correlation Spectroscopy

The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.

scholarly journals Design and development of paclitaxel-loaded bovine serum albumin nanoparticles for brain targeting

2011 ◽  
Vol 61 (2) ◽  
pp. 141-156 ◽  
Author(s):  
Amit Bansal ◽  
Deepak Kapoor ◽  
Rishi Kapil ◽  
Neha Chhabra ◽  
Sanju Dhawan
Keyword(s):  
Electron Microscopy ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Correlation Spectroscopy ◽  
Brain Targeting ◽  
Release Mechanism ◽  
Design And Development ◽  
Albumin Nanoparticles

Design and development of paclitaxel-loaded bovine serum albumin nanoparticles for brain targeting Bovine serum albumin (BSA) nanoparticles loaded with paclitaxel (PTX) were prepared using a desolvation technique. A 32 full factorial design (FFD) was employed to formulate nanoparticles. Nanoparticles were characterized for particle size by photon correlation spectroscopy and surface morphology by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Encapsulation efficiency, zeta potential and particle yield were also determined. Response surface linear modelling (RSLM) was used to predict the optimal formulation. Various models were applied to determine the release mechanism from PTX nanoparticles. The effect of drug-polymer ratio on the release profile of formulations was observed and was applied to determine the suitability of the predicted optimal formulation. A preliminary study to determine the feasibility of targeting the prepared nanoparticles to brain was also carried out using mice as in vivo models.

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