Spectrum–effect relationship between GC‐QTOF‐MS fingerprint and antioxidant, anti‐inflammatory activities of Schizonepeta tenuifolia essential oil

Farfarae Flos (FF) is the dried flower bud of Tussilago farfara L, which has antitussive, expectorant, and anti-inflammatory effects. However, little research on the main active composition of FF has been reported. The purpose of this study is to find the main active compounds responsible for the three pharmacological effects (i.e., antitussive, expectorant, and anti-inflammatory effects) of Farfarae Flos, based on the spectrum–effect relationship combined with chemometrics. First, this study uses the UPLC-QDA method to establish the chromatography fingerprint of Farfarae Flos, which is combined with chemometrics to analyze 18 batches of samples. Then, we study the antitussive, expectorant, and anti-inflammatory effects of Farfarae Flos. Finally, the spectrum–effect relationship between the fingerprint and the three pharmacological effects are studied by grey correlation analysis and partial least squares regression. The results show that four, four, and three main active constituents were found for the antitussive, expectorant, and anti-inflammatory pharmacological effects, respectively. In conclusion, we found the main active compounds corresponding to the main pharmacodynamic effects of Farfarae Flos. To our knowledge, this is the first time that spectrum–effect relationships in FF have been established using both raw and processed samples, which provides an experimental basis for further studies on the pharmacodynamic material basis of Farfarae Flos, as well as providing reference for the comprehensive evaluation of Farfarae Flos quality and the development of substitute resources.

Download Full-text

Identification of anti-inflammatory components of raw and steamed Panax notoginseng root by analyses of spectrum-effect relationship

RSC Advances ◽

10.1039/c9ra00906j ◽

2019 ◽

Vol 9 (31) ◽

pp. 17950-17958 ◽

Cited By ~ 1

Author(s):

Zejun Zhang ◽

Lijuan Chen ◽

Xiuming Cui ◽

Yiming Zhang ◽

Yupiao Hu ◽

...

Keyword(s):

Panax Notoginseng ◽

Effect Relationship ◽

Anti Inflammatory ◽

Spectrum Effect

The differentiated components related to the anti-inflammatory effects of raw and steamed Panax notoginseng were uncovered by the analysis of spectrum-effect relationship.

Download Full-text

Study on spectrum-effect relationship between fingerprint of essential oil and of anti-tumor effect from Curcuma kwangsiensis

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp11.840 ◽

2012 ◽

Vol 6 (18) ◽

Cited By ~ 2

Author(s):

Jianhong Zeng

Keyword(s):

Essential Oil ◽

Effect Relationship ◽

Curcuma Kwangsiensis ◽

Spectrum Effect

Download Full-text

Spectrum–effect relationship of antioxidant and anti‐inflammatory activities of Laportea bulbifera based on multivariate statistical analysis

Biomedical Chromatography ◽

10.1002/bmc.4734 ◽

2020 ◽

Vol 34 (2) ◽

Cited By ~ 1

Author(s):

Yinrui Chen ◽

Shuhan Zou ◽

Wenfen Xu ◽

Qingwen Sun ◽

Li Yun

Keyword(s):

Statistical Analysis ◽

Multivariate Statistical Analysis ◽

Multivariate Statistical ◽

Effect Relationship ◽

Anti Inflammatory ◽

Relationship Of ◽

Spectrum Effect

Download Full-text

Antifungal and anti-inflammatory claims for wild carrot essential oil

Planta Medica ◽

10.1055/s-0034-1394792 ◽

2014 ◽

Vol 80 (16) ◽

Author(s):

J Valente ◽

R Resende ◽

M Zuzarte ◽

MJ Gonçalves ◽

MC Lopes ◽

...

Keyword(s):

Essential Oil ◽

Wild Carrot ◽

Anti Inflammatory

Download Full-text

Comparative Analysis of In-Vitro Biological Activities of Methyl Eugenol Rich Cymbopogon khasianus Hack., Leaf Essential Oil with Pure Methyl Eugenol Compound

Current Pharmaceutical Biotechnology ◽

10.2174/1389201021666200217113921 ◽

2020 ◽

Vol 21 (10) ◽

pp. 927-938 ◽

Cited By ~ 7

Author(s):

Roktim Gogoi ◽

Rikraj Loying ◽

Neelav Sarma ◽

Twahira Begum ◽

Sudin K. Pandey ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Biological Activities ◽

Methyl Eugenol ◽

Dpph Assay ◽

Pure Compound ◽

Allium Cepa Assay ◽

Anti Inflammatory ◽

Lemongrass Essential Oil

Background: The essential oil of methyl eugenol rich Cymbopogon khasianus Hack. was evaluated and its bioactivities were compared with pure methyl eugenol. So far, methyl eugenol rich essential oil of lemongrass was not studied for any biological activities; hence, the present study was conducted. Objective: This study examined the chemical composition of essential oil of methyl eugenol rich Cymbopogon khasianus Hack., and evaluated its antioxidant, anti-inflammatory, antimicrobial, and herbicidal properties and genotoxicity, which were compared with pure compound, methyl eugenol. Material and Methods: Methyl eugenol rich variety of Cymbopogon khasianus Hack., with registration no. INGR18037 (c.v. Jor Lab L-9) was collected from experimental farm CSIR-NEIST, Jorhat, Assam (26.7378°N, 94.1570°E). The essential oil wasobtained by hydro-distillation using a Clevenger apparatus. The chemical composition of the essential oil was evaluated using GC/MS analysis and its antioxidant (DPPH assay, reducing power assay), anti-inflammatory (Egg albumin denaturation assay), and antimicrobial (Disc diffusion assay, MIC) properties, seed germination effect and genotoxicity (Allium cepa assay) were studied and compared with pure Methyl Eugenol compound (ME). Results: Major components detected in the Essential Oil (EO) through Gas chromatography/mass spectroscopy analysis were methyl eugenol (73.17%) and β-myrcene (8.58%). A total of 35components were detected with a total identified area percentage of 98.34%. DPPH assay revealed considerable antioxidant activity of methyl eugenol rich lemongrass essential oil (IC50= 2.263 μg/mL), which is lower than standard ascorbic acid (IC50 2.58 μg/mL), and higher than standard Methyl Eugenol (ME) (IC50 2.253 μg/mL). Methyl eugenol rich lemongrass EO showed IC50 38.00 μg/mL, ME 36.44 μg/mL, and sodium diclofenac 22.76 μg/mL, in in-vitro anti-inflammatory test. Moderate antimicrobial activity towards the 8 tested microbes was shown by methyl eugenol rich lemongrass essential oil whose effectiveness against the microbes was less as compared to pure ME standard. Seed germination assay further revealed the herbicidal properties of methyl eugenol rich essential oil. Moreover, Allium cepa assay revealed moderate genotoxicity of the essential oil. Conclusion: This paper compared the antioxidant, anti-inflammatory, antimicrobial, genotoxicity and herbicidal activities of methyl eugenol rich lemongrass with pure methyl eugenol. This methyl eugenol rich lemongrass variety can be used as an alternative of methyl eugenol pure compound. Hence, the essential oil of this variety has the potential of developing cost-effective, easily available antioxidative/ antimicrobial drugs but its use should be under the safety range of methyl eugenol and needs further clinical trials.

Download Full-text