Direct analysis of rat bile for acetaminophen and two of its conjugated metabolites via thermospray liquid chromatography/mass spectrometry

1987 ◽  
Vol 14 (12) ◽  
pp. 705-709 ◽  
Author(s):  
L. D. Betowski ◽  
W. A. Korfmacher ◽  
J. O. Lay ◽  
D. W. Potter ◽  
J. A. Hinson
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Direct Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Rat Bile ◽  
Conjugated Metabolites

Method development for the determination of 1,1-dimethylhydrazine by the high-performance liquid chromatography–mass spectrometry technique

2018 ◽  
Vol 24 (4) ◽  
pp. 352-359 ◽  
Author(s):  
Igors Susinskis ◽  
Peteris Mekss ◽  
Juris Hmelnickis
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Method Development ◽  
Direct Analysis ◽  
High Reactivity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
The Cost

Unsymmetrical dimethyl hydrazine is highly toxic, carcinogenic compound, widely used for organic synthesis and drug development. Therefore, due to its high reactivity, direct analysis is problematic. Current study proposes to use derivatization reaction to increase selectivity and sensitivity of high-performance liquid chromatography–mass spectrometry method. Different derivatization agents were tested and optimal reaction media was found. Derivatization was performed by using small amounts of reagents to lower the cost of analysis. The full validation of the method was performed and it can be used in a routine control in pharmaceutical analysis. Method sensitivity is 0.15 ppm, and linearity range is 0.15–2.70 ppm.

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