Host cell protein adsorption characteristics during protein a chromatography

2012 ◽  
Vol 28 (4) ◽  
pp. 1037-1044 ◽  
Author(s):  
Richard D. R. Tarrant ◽  
M. Lourdes Velez-Suberbie ◽  
Andrew S. Tait ◽  
C. Mark Smales ◽  
Daniel G. Bracewell
Keyword(s):  
Host Cell ◽  
Protein Adsorption ◽  
Protein A ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Adsorption Characteristics

The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody purification process

2012 ◽  
Vol 110 (1) ◽  
pp. 240-251 ◽  
Author(s):  
Catherine E.M. Hogwood ◽  
Andrew S. Tait ◽  
Nadejda Koloteva-Levine ◽  
Daniel G. Bracewell ◽  
C. Mark Smales
Keyword(s):  
Host Cell ◽  
Protein Profile ◽  
Protein A ◽  
Cell Protein ◽  
Purification Process ◽  
Host Cell Protein ◽  
Antibody Purification

scholarly journals Residual on column host cell protein analysis during lifetime studies of protein A chromatography

2016 ◽  
Vol 1461 ◽  
pp. 70-77 ◽  
Author(s):  
Katherine Lintern ◽  
Mili Pathak ◽  
C. Mark Smales ◽  
Kevin Howland ◽  
Anurag Rathore ◽  
...  
Keyword(s):  
Host Cell ◽  
Protein A ◽  
Protein Analysis ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Lifetime Studies

Studying host cell protein interactions with monoclonal antibodies using high throughput protein A chromatography

2012 ◽  
Vol 7 (10) ◽  
pp. 1233-1241 ◽  
Author(s):  
Vikram N. Sisodiya ◽  
Joshua Lequieu ◽  
Maricel Rodriguez ◽  
Paul McDonald ◽  
Kathlyn P. Lazzareschi
Keyword(s):  
Monoclonal Antibodies ◽  
Host Cell ◽  
High Throughput ◽  
Protein Interactions ◽  
Protein A ◽  
Cell Protein ◽  
Host Cell Protein

scholarly journals The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Nicholas M. Negretti ◽  
Christopher R. Gourley ◽  
Prabhat K. Talukdar ◽  
Geremy Clair ◽  
Courtney M. Klappenbach ◽  
...  
Keyword(s):  
Host Cell ◽  
Campylobacter Jejuni ◽  
Foodborne Pathogen ◽  
Small Gtpase ◽  
Host Cells ◽  
Cell Protein ◽  
Host Cell Protein ◽  
Cell Binding ◽  
Actin Reorganization ◽  
Cell Cytosol

AbstractCampylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrained activity of the small GTPase Rac1, which is known to have roles in actin reorganization and internalization of C. jejuni. Our results represent the identification of a host cell protein targeted by a flagellar secreted effector protein and demonstrate that C. jejuni-stimulated Rac signaling is dependent on IQGAP1.

Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics

2016 ◽  
Vol 18 (6) ◽  
pp. 1439-1452 ◽  
Author(s):  
Vibha Jawa ◽  
Marisa K. Joubert ◽  
Qingchun Zhang ◽  
Meghana Deshpande ◽  
Suminda Hapuarachchi ◽  
...  
Keyword(s):  
Host Cell ◽  
Cell Protein ◽  
Host Cell Protein
Sign in / Sign up

Export Citation Format

Share Document