High-throughput screening techniques for rapid PEG-based precipitation of IgG4 mAb from clarified cell culture supernatant

2009 ◽  
Vol 26 (3) ◽  
pp. 697-705 ◽  
Author(s):  
Carol Knevelman ◽  
Jim Davies ◽  
Lee Allen ◽  
Nigel J. Titchener-Hooker
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
High Throughput Screening ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Screening Techniques

High-throughput quantitation of Fc-containing recombinant proteins in cell culture supernatant by fluorescence polarization spectroscopy

2017 ◽  
Vol 534 ◽  
pp. 49-55 ◽  
Author(s):  
Ben Thompson ◽  
Jerry Clifford ◽  
Mike Jenns ◽  
Andrew Smith ◽  
Ray Field ◽  
...  
Keyword(s):  
Cell Culture ◽  
Recombinant Proteins ◽  
High Throughput ◽  
Fluorescence Polarization ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Polarization Spectroscopy

Comparison among three anion exchange chromatographic supports to capture erythropoietin from cell culture supernatant Lourdes

2015 ◽  
Vol 33 (6) ◽  
pp. 642 ◽  
Author(s):  
HERNÁNDEZ Lourdes ◽  
STEWART Diobel ◽  
ZUMALACÁRREGUI Lourdes ◽  
AMARO Daniel
Keyword(s):  
Cell Culture ◽  
Anion Exchange ◽  
Culture Supernatant ◽  
Cell Culture Supernatant

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

2013 ◽  
Vol 8 (3) ◽  
pp. 352-362 ◽  
Author(s):  
Paula A. J. Rosa ◽  
Ana M. Azevedo ◽  
S. Sommerfeld ◽  
Martina Mutter ◽  
Werner Bäcker ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

An ultrasensitive immunoassay for human IgE measurement in cell-culture supernatant

1990 ◽  
Vol 26 (3) ◽  
pp. 283-284 ◽  
Author(s):  
R.G. Vasilov ◽  
E.N. Tsitsikov
Keyword(s):  
Cell Culture ◽  
Culture Supernatant ◽  
Cell Culture Supernatant

scholarly journals Development of NS3/4A Protease-Based Reporter Assay Suitable for Efficiently Assessing Hepatitis C Virus Infection

2009 ◽  
Vol 53 (11) ◽  
pp. 4825-4834 ◽  
Author(s):  
Kao-Lu Pan ◽  
Jin-Ching Lee ◽  
Hsing-Wen Sung ◽  
Teng-Yuang Chang ◽  
John T.-A. Hsu
Keyword(s):  
Cell Culture ◽  
Life Cycle ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Infection ◽  
High Throughput ◽  
High Throughput Screening ◽  
Reporter System ◽  
Viral Protease ◽  
Peptide Cleavage

ABSTRACT A cell culture system for the production of hepatitis C virus (HCV) whole virions has greatly accelerated studies of the virus life cycle and the discovery of anti-HCV agents. However, the quantification of the HCV titers in a whole-virus infection/replication system currently relies mostly on reverse transcription-PCR or immunofluorescence assay, which would be cumbersome for high-throughput drug screening. To overcome this problem, this study has generated a novel cell line, Huh7.5-EG(Δ4B5A)SEAP, that carries a dual reporter, EG(Δ4B5A)SEAP. The EG(Δ4B5A)SEAP reporter is a viral protease-cleavable fusion protein in which the enhanced green fluorescence protein is linked to secreted alkaline phosphatase (SEAP) in frame via Δ4B5A, a short peptide cleavage substrate for NS3/4A viral protease. This study demonstrates that virus replication/infection in the Huh7.5-EG(Δ4B5A)SEAP cells can be quantitatively indicated by measuring the SEAP activity in cell culture medium. The levels of SEAP released from HCV-infected Huh7.5-EG(Δ4B5A)SEAP cells correlated closely with the amounts of HCV in the inocula. The Huh7.5-EG(Δ4B5A)SEAP cells were also shown to be a suitable host for the discovery of anti-HCV inhibitors by using known compounds that target multiple stages of the HCV life cycle. The Z′-factor of this assay ranged from 0.64 to 0.74 in 96-well plates, indicating that this reporter system is suitable for high-throughput screening of prospective anti-HCV agents.

Patterned superhydrophobic surfaces to process and characterize biomaterials and 3D cell culture

2018 ◽  
Vol 5 (3) ◽  
pp. 379-393 ◽  
Author(s):  
A. I. Neto ◽  
P. A. Levkin ◽  
J. F. Mano
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
High Throughput Screening ◽  
3D Cell Culture ◽  
Superhydrophobic Surfaces ◽  
Technological Breakthrough ◽  
Large Numbers

Microarrays are a technological breakthrough for high-throughput screening of large numbers of assays.

Precipitation of VEGF from mesenchymal stem cell culture supernatant using the PCA process

2019 ◽  
Vol 151 ◽  
pp. 40-48 ◽  
Author(s):  
Hee Jeong Park ◽  
Tae Jun Yoon ◽  
Won-Su Son ◽  
Chan-Ju Lee ◽  
Si-na Kim ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Culture Supernatant ◽  
Cell Culture Supernatant ◽  
Mesenchymal Stem Cell Culture ◽  
Stem Cell Culture
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