Dietary supplements based on modified and enriched with amino acids zeolite in the cultivation of young turkeys

Relevance. Modern turkey crosses have a genetically determined high growth rate. These farm birds are very sensitive to even minor fluctuations in their diet, are susceptible to a deficiency of amino acids, protein, mineral elements and vitamins, which can contribute to a decrease in their productivity, the development of pathological processes and diseases. The use of modern technologies and scientific achievements has made it possible to prepare a new product — modified zeolite. This natural mineral is already free of impurities and microbes, with open pore windows, has a high ability to selectively exchange, and is obtained by various methods of activating zeolite in industrial conditions. At the same time, Ulyanovsk specialists have mastered the technology of enriching modified zeolite with useful substances.Methods. The aim of the work was to study the effect on the body of turkeys and the level of their productivity of additives based on modified zeolite enriched with amino acids. The experiment was organized for 60 days on 250 turkeys of the breed Hybrid Cread-mayker aged 55–60 days in the conditions of the Ulyanovsk region. The control group received the main ration (MR), the experimental group was given once a day in addition to the MR in the morning feeding in a mixture with mixed feed 5% additives of modified zeolite enriched with amino acids.Results. The inclusion of a supplement based on modified zeolite enriched with amino acids in the diet of turkeys does not have a negative effect on the hematological parameters of their blood, all indicators corresponded to the physiological norm. During the experiment in the 2nd group, the live weight of poultry was obtained larger by 8.70%, which amounted to 12,296 kg, respectively, more profit was obtained from the sale of turkey meat, total revenue was 133,210, and additional profit was 27,960 rubles, 2.41 rubles of profit was received for 1 ruble of costs. Тhe data indicate the normalization of mineral homeostasis in the body of turkeys and better absorption of nutrients in the feed diet.

One Year Monitoring of Ecological Interaction of Silurus glanis in a Novel Invaded Oligotrophic Deep Lake (Lake Maggiore)

The European catfish (Silurus glanis) was introduced in Italy during the last century for aquaculture purposes, and now it is well-established. S. glanis is an invasive species and a top predator that can deplete prey supply in the surrounding habitat, leading to changes in the aquatic food web. Consequently, its presence is considered a threat to native fish populations. Its presence in the Lake Maggiore (Northern Italy) is recent and there is a lack of knowledge about its ability to completely exploit this new ecosystem throughout the year. This study corroborated the ability of European catfish to exploit both pelagic and littoral habitats, promoting trophic interactions in both habitats. Over 2019, multiple sampling approaches have been applied by collecting S. glanis and analysing its stomach contents with the aim of inferring interactions with the freshwater community. Its diet was mainly based on crayfish (Orconectes limosus), followed by six prey fish and the genus Corbicula; two fish species (Padogobius bonelli and Salaria fluviatilis) were added to the list of known prey fish. Notably and alarmingly for the early potential top-down pressure towards all trophic levels, young individuals were proved to also feed on fish and crayfish. S. glanis showed the ability to hunt at deep depths (>60 m) and a high growth rate, despite Lake Maggiore being oligotrophic.

Hairy Roots

Agrobacterium rhizogenes induces hairy root disease in plants. The neoplastic (cancerous) roots produced by A. rhizogenes infection, when cultured in hormone free medium, show high growth rate and genetic stability. These genetically transformed root cultures can produce levels of secondary metabolites comparable to that of intact plants. Several elicitation methods can be used to further enhance the production and accumulation of secondary metabolites. Thus, hairy root culture offer promise for high production and productivity of valuable secondary metabolites in many plants. Hairy roots can also produce recombinant proteins from transgenic roots, and thereby hold immense potential for pharmaceutical industry. Hairy root cultures can be used to elucidate the intermediates and key enzymes involved in the biosynthesis of secondary metabolites, and for phytoremediation due to their abundant neoplastic root proliferation property. Various applications of hairy root cultures and potential problems associated with them are discussed in this chapter.

Assessment of Breeding Practice and Statues of Estrus Synchronization and Mass Insemination Conception Rate of Dairy Cattle in North Shewa Zone, Ethiopia.

BackgroundAssisted reproductive biotechnology like oestrus synchronization mass insemination(OSMI), and artificial insemination (AI) are the most important bio techniques for improving the reproductive and productive performance of dairy cattle including enhancing overall profit in Ethiopia. In North Shewa zone different study were conducted. However, there is no study conducted on breeding practice, and status of OSMI conception rate of dairy cattle. Therefore the aim of this study to assess breeding practice, and status of OSMI conception rate of dairy cattle in North Shewa zone.MethodsOut of 27 districts, three district and 135 respondents were selected purposive followed by random sampling techniques per each district. Data were analyzed using SPSS version 20 and Ms-Excel (2010).ResultMilk yield, High growth rate, body weight, fertility and udder size were the major traits perceived by farmers. Breed preference of the respondents were HHFC and HFC in Basonaworena and Angolelanatera ranked first. Breeding objective and rearing system of cattle were milk production with sale of calves and all cattle categories reared together except HHFC and lactating cows respectively. Most of the respondents were used AI mating system due to rapid genetic improvement. HHFC and HJERC bulls breed were not available in the study area as a result alternative strategies taken by the respondents was take cows in other kebele. Reproductive performance of dairy cattle per district and breeds were statistically significant. 86.6% of respondents were not maintained mating and pedigree records due to lack of awareness. Heat detection problem and AIT efficiency were the major factor that affect CR in OSMI program. The perception and satisfaction of farmer on CR of OSMI (34.4%) was not good and (67.1%) not satisfied respectively. The selection criteria of cows for OSMI program (58.9%) of respondents were not aware. The status of CR and NSPC per district, breed and year were vary (p<0.05) in table16 in OSMI program.ConclusionIn conclusion that the status of CR was increasing starting 2013/14-2015/16 in OSMI. In addition creation of farmer’s awareness on breeding aspects as well as OSMI is mandatory. Finally empowering the AI technician efficiency and procurement of the necessary facilities should be in place before implementing an OSMI.

Peculiarities of the disease and prevalence of chronic cystitis among the female population of Ukraine in the regional aspect

The study uses data from state and industry official statistics for 2008-2017. Absolute and relative indicators of morbidity and prevalence of chronic cystitis among the female population of Ukraine, its regions and areas were analyzed taking into account two five-year periods for comparative assessment of the nature and intensity of dynamic processes. It is revealed that the number of patients with chronic cystitis (СC) registered in Ukraine is at the expense of women, which are 3-3.5 times more in number than men, with their characteristic more intensive growth (for 10 years by 3.6% against 0.4% among the adult population in the country). The first three places in the structure belong to the Southeastern region, Kyiv, Western region, the next – Central, Southern, Northeastern regions. Levels of the prevalence of the disease among women (100 thousand) are higher than the average in Ukraine and have a high growth rate (for 10 years by 13.5% from 232.2 to 263.6 against 9.3% from 171.5 to 187.5, respectively). Typical for Ukraine persistent increase in patients with the first diagnosis of chronic cystitis (0.8%, 2.1% and 2.8%, respectively, in the first, second periods and 10 years to 15112 in 2017) is also formed by this category (women's growth was 3.4%, 12.4%, and 5.0%, respectively, to 11.295). A similar situation was also identified in the analysis of the level of morbidity (per 100 thousand). In Ukraine, its growth rate for the last five years was 9.6% against 1.8% for the previous year, for 10 years – 11.9%, and the value reached 43.4 in 2017 against 38.8 in 2008. Among women, its levels are higher than the average in Ukraine (in 2017 – 59.2 against 56.1 in 2008), and the increase was more intense (by 6.2% and 11.3% over the periods; for 10 years – by 11.98%).

The structure of productivity elements of durum wheat varieties of Saratov selection

The objects of the study were plants of 14 varieties of durum wheat Triticum durum Desf., permitted for use at different times in the period from 1975 to 2014. An analysis of the elements of ear productivity in durum wheat plants of Saratov varieties under the conditions of 2020 was carried out. The varietal features of the development of the spike of the main shoot were revealed according to a number of characteristics: the number of spikelets in a spike, the number of grains in an ear, the weight of a grain, the number of ungrained and grained spikelets. The height of the plant and the length of the spike were measured. On the basis of the data obtained, the selection indices were calculated – the Canadian index, the Mexican index, the index of the linear density of the ear, the morphogenetic index of productivity. A cultivar with a balanced type of morphogenetic systems was revealed in terms of the elements of ear productivity – the number of spikelets, the number of caryopses and their weight – Luch 25. This cultivar is characterized by the most developed embryonic shoot among the studied cultivars, as well as the maximum length of the embryonic root system and a high growth rate root system of the seedling.

Exploring environmental connection: ecological network opportunities in the Shahdagh National Park, Azerbaijan

The high growth rate of consumption of natural resources by people in the last century have seriously and negatively affected ecosystems and led to the loss of biodiversity. At present, it is important to take the necessary measures to ensure sustainable development and biodiversity conservation. Ecological networks play an important role in maintaining and controlling the stability and balance of communication between ecosystem complexes. For this purpose, the possibility of creating an ecological network that includes core areas, buffer zones, ecological corridors and restoration areas in the section of Shamakhi district of the Shahdagh National Park was explored. The designated buffer zone of the national park includes 14 villages and 1 settlement type administrative unit. Mountainous landscapes predominate in the area, and the absolute height ranges from 500-200 m. Riverbeds, intermountain depression, mountain passes, trails and forests which should be planted in the areas we offer, were considered as main factors during the construction of ecological corridors in the national park and the buffer zones. The materials obtained from the literature and our field studies, also, vector and raster data were used during the site identification. The data was processed in software such as ArcGIS 10, ERDAS Imagine, Global Mapper, Google Earth, etc. Existing ecosystems were identified, and areas were calculated in separate elements of the ecological network in the study area.

Evaluation of Antioxidant and Antifungal Activity of the Whole Part of Dactyloctenium aegyptium Weed on Bengal Gram

Background: This study was conducted about the effectiveness of weed Dactyloctenium aegyptium. Weedsare familiar dominant, redundant, adverse and weed that contend with sophisticated crop for water, nutrient and sunlight and another several reasons such as, high growth rate, high reproductive rate and produce harmful or beneficial allelopathical effect of cultivated crops. The stems of Dactyloctenium aegyptium are willowy, geniculate and leaves are found roughly. Methods: This Investigation was done in 2018-19 to 2020-21. Different part of Dactyloctenium aegyptium as leaves and seed was taken from the near area of Sardar Vallabh Bhai Patel Agriculture and Technology University Modipuram Meerut. The samples were shade dry for 24 to 48 hours and then grind in the powder form. The extract were prepare in different organic solvent as Methanol, Ethyl acetate, Butyl alcohol, Benzene and Water at 1:10 ratio. Antioxidant activity of weed extracts by three methods named DPPH FRAP and ABTS methods. Result: All part of this weed show effectiveness due to the presence of active compound, who responsible for the positive result. Extract mixed with media at a particular concentration i.e. 5%, 10% and 15% show effect on soil borne fungal phytopathogens and then over the surface of petriplate the growth was appear reduce when we increase the concentration of extract. At the end we conclude that the 15% extract concentration of both part of Dactyloctenium reduce the growth of all the soil borne fungal phytopathogens.

Influence of prefoldin subunit 4 on the tolerance of Kluyveromyces marxianus to lignocellulosic biomass-derived inhibitors

Background Kluyveromyces marxianus is a potentially excellent host for microbial cell factories using lignocellulosic biomass, due to its thermotolerance, high growth rate, and wide substrate spectrum. However, its tolerance to inhibitors derived from lignocellulosic biomass pretreatment needs to be improved. The prefoldin complex assists the folding of cytoskeleton which relates to the stress tolerance, moreover, several subunits of prefoldin have been verified to be involved in gene expression regulation. With the presence of inhibitors, the expression of a gene coding the subunit 4 of prefoldin (KmPFD4), a possible transcription factor, was significantly changed. Therefore, KmPFD4 was selected to evaluate its functions in inhibitors tolerance. Results In this study, the disruption of the prefoldin subunit 4 gene (KmPFD4) led to increased concentration of intracellular reactive oxygen species (ROS) and disturbed the assembly of actin and tubulin in the presence of inhibitors, resulting in reduced inhibitor tolerance. Nuclear localization of KmPFD4 indicated that it could regulate gene expression. Transcriptomic analysis showed that upregulated gene expression related to ROS elimination, ATP production, and NAD+ synthesis, which is a response to the presence of inhibitors, disappeared in KmPFD4-disrupted cells. Thus, KmPFD4 impacts inhibitor tolerance by maintaining integration of the cytoskeleton and directly or indirectly affecting the expression of genes in response to inhibitors. Finally, overexpression of KmPFD4 enhanced ethanol fermentation with a 46.27% improvement in productivity in presence of the inhibitors. Conclusion This study demonstrated that KmPFD4 plays a positive role in the inhibitor tolerance and can be applied for the development of inhibitor-tolerant platform strains.