scholarly journals Gene Expression Profiling on Global cDNA Arrays Gives Hints Concerning Potential Signal Transduction Pathways Involved in Cardiac Fibrosis of Renal Failure

2003 ◽  
Vol 4 (6) ◽  
pp. 571-583 ◽  
Author(s):  
Kerstin Amann ◽  
Heidrun Ridinger ◽  
Christiane Rutenberg ◽  
Eberhard Ritz ◽  
Gerhard Mall ◽  
...  
Keyword(s):  
Gene Expression ◽  
Renal Failure ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Cardiac Remodelling ◽  
Cardiomyocyte Hypertrophy ◽  
Specific Gene ◽  
Interstitial Tissue ◽  
Sham Operation ◽  
Cdna Arrays

Cardiac remodelling with interstitial fibrosis in renal failure, which so far is only poorly understood on the molecular level, was investigated in the rat model by a global gene expression profiling analysis. Sprague–Dawley rats were subjected to subtotal nephrectomy (SNX) or sham operation (sham) and followed for 2 and 12 weeks, respectively. Heart-specific gene expression profiling, with RZPD Rat Unigene-1 cDNA arrays containing about 27 000 gene and EST sequences revealed substantial changes in gene expression in SNX compared to sham animals. Motor protein genes, growth and differentiation markers, and extracellular matrix genes were upregulated in SNX rats. Obviously, not only genes involved in cardiomyocyte hypertrophy, but also genes involved in the expansion of non-vascular interstitial tissue are activated very early in animals with renal failure. Together with earlier findings in the SNX model, the present data suggest the hypothesis that the local renin–angiotensin system (RAS) may be activated by at least two pathways: (a) via second messengers and Gproteins (short-term signalling); and (b) via motor proteins, actins and integrins (longterm signalling). The study documents that complex hybridization analysis yields reproducible and promising results of patterns of gene activation pointing to signalling pathways involved in cardiac remodelling in renal failure. The complete array data are available via http://www.rzpd.de/cgi-bin/services/exp/viewExpressionData.pl.cgi

Gene expression profiling of human satellite cells during muscular aging using cDNA arrays

Gene ◽  
2003 ◽  
Vol 321 ◽  
pp. 145-154 ◽  
Author(s):  
Sylvie Bortoli ◽  
Valérie Renault ◽  
Eric Eveno ◽  
Charles Auffray ◽  
Gill Butler-Browne ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Satellite Cells ◽  
Expression Profiling ◽  
Cdna Arrays

scholarly journals Gene expression profiling of the Notch-AhR-IL22 axis at homeostasis and in response to tissue injury

2017 ◽  
Vol 37 (6) ◽  
Author(s):  
Marc Weidenbusch ◽  
Severin Rodler ◽  
Shangqing Song ◽  
Simone Romoli ◽  
Julian A. Marschner ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Tissue Injury ◽  
Expression Profiles ◽  
Notch Pathway ◽  
Gene Expression Profiles ◽  
Sterile Inflammation ◽  
Specific Gene ◽  
Specific Gene Expression

Notch and interleukin-22 (IL-22) signaling are known to regulate tissue homeostasis and respond to injury in humans and mice, and the induction of endogenous aryl hydrocarbon receptor (Ahr) ligands through Notch links the two pathways in a hierarchical fashion. However in adults, the species-, organ- and injury-specific gene expression of the Notch-AhR-IL22 axis components is unknown. We therefore performed gene expression profiling of DLL1, DLL3, DLL4, DLK1, DLK2, JAG1, JAG2, Notch1, Notch2, Notch3, Notch4, ADAM17/TNF-α ADAM metalloprotease converting enzyme (TACE), PSEN1, basigin (BSG)/CD147, RBP-J, HES1, HES5, HEY1, HEYL, AHR, ARNT, ARNT2, CYP1A1, CYP24A1, IL-22, IL22RA1, IL22RA2, IL10RB, and STAT3 under homeostatic conditions in ten mature murine and human organs. Additionally, the expression of these genes was assessed in murine models of acute sterile inflammation and progressive fibrosis. We show that there are organ-specific gene expression profiles of the Notch-AhR-IL22 axis in humans and mice. Although there is an overall interspecies congruency, specific differences between human and murine expression signatures do exist. In murine tissues with AHR/ARNT expression CYP1A1 and IL-22 were correlated with HES5 and HEYL expression, while in human tissues no such correlation was found. Notch and AhR signaling are involved in renal inflammation and fibrosis with specific gene expression changes in each model. Despite the presence of all Notch pathway molecules in the kidney and a model-specific induction of Notch ligands, IL-22 was only up-regulated in acute inflammation, but rapidly down-regulated during regeneration. This implies that for targeting injury responses, e.g. via IL-22, species-specific differences, injury type and time points have to be considered.

scholarly journals Novel role of AGT gene in aplastic anaemia among paediatric patients based on gene expression profiling

2020 ◽  
Author(s):  
Sarmistha Adhikari ◽  
PARAMITA MANDAL
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Aplastic Anemia ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Patient Specific ◽  
Specific Gene ◽  
Healthy Controls ◽  
Agt Gene

Abstract ObjectivesSevere aplastic anemia is characterized by a hypocellular bone marrow and peripheral cytopenia. Mesenchymal stem cells (MSCs) play a crucial role in haematopoietic stem cells (HSCs) development and the microenvironment suitable for haematopoiesis. Investigation of the therapeutic targets by paediatric patient-specific gene expression analysis of the MSCs can be important for diagnosis.MethodsThe study was based on freely available miRNA and host gene expression in NCBI GEO dataset. Microarray based gene expression profiles (GSE33812) of MSCs for five paediatric aplastic anaemia patients and healthy controls were generated using Agilent-014850 platform and the data was downloaded from the database.ResultsMSCs gene expression profiling distinguished between healthy controls, children with aplastic anemia. Angioteninogen (AGT) gene involved in ERK1/ERK2 cascade, cyotokine secretion, metabolic processes was strongly down-regulated among all the patients with aplastic anemia. Emerging role of various transcription factors binding to this gene was identified as a new avenue of therapeutic application.ConclusionsAs a potential diagnostic tool, patient-specific gene expression profiling of MSCs made it possible to make the difficult diagnosis of most patients with aplastic anemia.

scholarly journals Breed, sex and anatomical location-specific gene expression profiling of the porcine skeletal muscles

BMC Genetics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 53 ◽  
Author(s):  
Jie Zhang ◽  
Chaowei Zhou ◽  
Jideng Ma ◽  
Lei Chen ◽  
Anan Jiang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Skeletal Muscles ◽  
Specific Gene ◽  
Anatomical Location ◽  
Specific Gene Expression
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