ChemInform Abstract: NUCLEIC ACID COMPONENTS AND THEIR ANALOGUES PART 163, AMINO ACIDS AND PEPTIDES PART 119, S-(PYRIMIDIN-2-YL)-L-CYSTEINE, CHEMICAL SYNTHESIS AND BIOSYNTHESIS IN ESCHERICHIA COLI

1974 ◽  
Vol 5 (18) ◽  
pp. no-no
Author(s):  
A. HOLY ◽  
I. VOTRUBA ◽  
K. JOST
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Nucleic Acid ◽  
Chemical Synthesis

Ultraviolet Resonance Raman Spectra of Escherichia Coli with 222.5–251.0 nm Pulsed Laser Excitation

1988 ◽  
Vol 42 (5) ◽  
pp. 782-788 ◽  
Author(s):  
K. A. Britton ◽  
R. A. Dalterio ◽  
W. H. Nelson ◽  
D. Britt ◽  
J. F. Sperry
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Nucleic Acid ◽  
Raman Spectra ◽  
Resonance Raman ◽  
Aromatic Amino Acids ◽  
Selective Excitation ◽  
Exciting Wavelength ◽  
Resonance Raman Spectra ◽  
Ultraviolet Resonance Raman

Resonance Raman spectra of the gram-negative organism, Escherichia coli, have been obtained with 222.5-, 230.6-, and 251.0-nm excitation, and the results have been compared with those reported earlier for 242.4-nm excitation. Major changes in bacterial spectra have been observed with changes in exciting wavelength. The origins of the major peaks in each spectrum have been explained primarily in terms of contributions of nucleic acid bases and aromatic amino acids. As an aid in making assignments, spectra of aromatic amino acids, nucleosides, and mixtures of the two have been obtained at each wavelength used to excite bacterial spectra. Background fluorescence has been observed to be negligible below 251 nm. Selective excitation of bacterial nucleic acid and protein components has been done with ease. Results suggest that an extension of the exciting wavelength range to 190–220 nm will allow the selective excitation of additional cell components.

Capacité de la méthionine, de la thiamine ou du pantothénate à renverser la toxicité, pour Escherichia coli, d'acides α-aminés artificiels homologues, dont la norleucine; mise en evidence du rôle probable de la méthionine dans la biosynthèse des deux vitamines

1973 ◽  
Vol 51 (5) ◽  
pp. 673-685
Author(s):  
G. Planet ◽  
C. J. Abshire
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Chemical Synthesis ◽  
Linear Groups ◽  
Biosynthetic Enzymes ◽  
E Coli ◽  
Mechanism Of Toxicity ◽  
Methionine Pathway

We have previously made the chemical synthesis of artificial α-amino acids substituted by one or two alkylated linear groups on the α-carbon. Our present results indicate that they have a capacity to stop the growth of E. coli 9723. When they have toxicity, the inhibition is competitively reversed by L-methionine and noncompetitively by pantothenate or thiamine; these compounds act as methionine analogues. We have concluded that the mechanism of toxicity is due in part to the repression of biosynthetic enzymes of the methionine pathway and partly to the inhibition of homoserine-O-transsuccinylase, which is the first enzyme of this pathway. We think that the consequence is an intracellular deficiency of methionine which, in turn, causes a lack of pantothenate and thiamine. Our results, therefore, indicate that methionine is necessary for the biosynthesis of pantothenate and thiamine.

Infection of Escherichia coli with bacteriophages

1969 ◽  
Vol 27 ◽  
pp. 370-371
Author(s):  
Manfred E. Bayer
Keyword(s):  
Escherichia Coli ◽  
Nucleic Acid ◽  
Cell Surface ◽  
Virus Type ◽  
Infection Process ◽  
Adsorption Site ◽  
The Other ◽  
Specific Receptors ◽  
Bacterial Receptors

The first step in the infection of a bacterium by a virus consists of a collision between cell and bacteriophage. The presence of virus-specific receptors on the cell surface will trigger a number of events leading eventually to release of the phage nucleic acid. The execution of the various "steps" in the infection process varies from one virus-type to the other, depending on the anatomy of the virus. Small viruses like ØX 174 and MS2 adsorb directly with their capsid to the bacterial receptors, while other phages possess attachment organelles of varying complexity. In bacteriophages T3 (Fig. 1) and T7 the small conical processes of their heads point toward the adsorption site; a welldefined baseplate is attached to the head of P22; heads without baseplates are not infective.

Microbe Engineering of Guaia-6,10(14)-diene as a Building Block for the Semisynthetic Production of Plant-Derived (−)-Englerin A

2019 ◽  
Author(s):  
Thomas Siemon ◽  
Zhangqian Wang ◽  
Guangkai Bian ◽  
Tobias Seitz ◽  
Ziling Ye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Synthetic Biology ◽  
Chemical Synthesis ◽  
Building Block ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Economical Method ◽  
Englerin A ◽  
Transient Receptor

Herein, we report the semisynthetic production of the potent transient receptor potential canonical (TRPC) channel agonist (−)-englerin A (EA), using guaia-6,10(14)-diene as the starting material. Guaia-6,10(14)-diene was systematically engineered in Escherichia coli and Saccharomyces cerevisiae using the CRISPR/Cas9 system and produced with high titers. This provided us the opportunity to execute a concise chemical synthesis of EA and the two related guaianes (−)-oxyphyllol and (+)-orientalol E. The potentially scalable approach combines the advantages of synthetic biology and chemical synthesis and provides an efficient and economical method for producing EA as well as its analogs.

Microbe Engineering of Guaia-6,10(14)-diene as a Building Block for the Semisynthetic Production of Plant-Derived (−)-Englerin A

2019 ◽  
Author(s):  
Thomas Siemon ◽  
Zhangqian Wang ◽  
Guangkai Bian ◽  
Tobias Seitz ◽  
Ziling Ye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Synthetic Biology ◽  
Chemical Synthesis ◽  
Building Block ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Economical Method ◽  
Englerin A ◽  
Transient Receptor

Herein, we report the semisynthetic production of the potent transient receptor potential canonical (TRPC) channel agonist (−)-englerin A (EA), using guaia-6,10(14)-diene as the starting material. Guaia-6,10(14)-diene was systematically engineered in Escherichia coli and Saccharomyces cerevisiae using the CRISPR/Cas9 system and produced with high titers. This provided us the opportunity to execute a concise chemical synthesis of EA and the two related guaianes (−)-oxyphyllol and (+)-orientalol E. The potentially scalable approach combines the advantages of synthetic biology and chemical synthesis and provides an efficient and economical method for producing EA as well as its analogs.

scholarly journals ANTIPODAL SPECIFICITY IN THE INHIBITION OF GROWTH OF ESCHERICHIA COLI BY AMINO ACIDS

1948 ◽  
Vol 174 (2) ◽  
pp. 391-398
Author(s):  
Yutaka. Kobayashi ◽  
Marguerite. Fling ◽  
Sidney W. Fox
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Inhibition Of Growth
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