Rapid Determination of Costunolide and Dehydrocostuslactone in Human Plasma Sample and Chinese Patent Medicine Xiang Sha Yang Wei Capsule Using HPLC-DAD Coupled with Second-order Calibration

2012 ◽  
Vol 30 (5) ◽  
pp. 1137-1143 ◽  
Author(s):  
Yajuan Liu ◽  
Hailong Wu ◽  
Shaohua Zhu ◽  
Chao Kang ◽  
Hui Xu ◽  
...  
Keyword(s):  
Human Plasma ◽  
Plasma Sample ◽  
Rapid Determination ◽  
Second Order ◽  
Human Plasma Sample ◽  
Chinese Patent Medicine ◽  
Chinese Patent ◽  
Second Order Calibration ◽  
Patent Medicine

scholarly journals Micellar Enhanced Three-Dimensional Excitation-Emission Matrix Fluorescence for Rapid Determination of Antihypertensives in Human Plasma with Aid of Second-Order Calibration Methods

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Hai-Yan Fu ◽  
He-Dong Li ◽  
Chuang Ni ◽  
Tian-Ming Yang ◽  
Yao Fan ◽  
...  
Keyword(s):  
Human Plasma ◽  
Rapid Determination ◽  
Confidence Region ◽  
Three Dimensional ◽  
Second Order ◽  
Amlodipine Besylate ◽  
Promising Alternative ◽  
Calibration Methods ◽  
Second Order Calibration

A highly sensitive three-dimensional excitation-emission fluorescence method was proposed to determine antihypertensives including valsartan and amlodipine besylate in human plasma with the aid of second-order calibration methods based on parallel factor analysis (PARAFAC) and alternating trilinear decomposition (ATLD) algorithms. Antihypertensives with weak fluorescent can be transformed into a strong fluorescent property by changing microenvironment in samples using micellar enhanced surfactant. Both the adopted algorithms with second-order advantage can improve the resolution and directly attain antihypertensives concentration even in the presence of potential strong intrinsic fluorescence from human plasma. The satisfactory results can be achieved for valsartan and amlodipine besylate in complicated human plasma. Furthermore, some statistical parameters and figures of merit were evaluated to investigate the performance of the proposed method, and the accuracy and precision of the proposed method were also validated by the elliptical joint confidence region (EJCR) test and repeatability analysis of intraday and interday assay. The proposed method could not only light a new avenue to directly determine valsartan or amlodipine besylate in human plasma, but also hold great potential to be extended as a promising alternative for more practical applications in the determination of weak fluorescent drugs.

scholarly journals Simultaneous and Direct Determination of Vancomycin and Cephalexin in Human Plasma by Using HPLC-DAD Coupled with Second-Order Calibration Algorithms

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Le-Qian Hu ◽  
Chun-Ling Yin ◽  
Ya-Hui Du ◽  
Zhi-Peng Zeng
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Complex Analysis ◽  
Direct Determination ◽  
Second Order ◽  
Decomposition Algorithms ◽  
Statistical Comparison ◽  
Trilinear Decomposition ◽  
Second Order Calibration

A simple, rapid, and sensitive method for the simultaneous determination of vancomycin and cephalexin in human plasma was developed by using HPLC-DAD with second-order calibration algorithms. Instead of a completely chromatographic separation, mathematical separation was performed by using two trilinear decomposition algorithms, that is, PARAFAC-alternative least squares (PARAFAC-ALSs) and self-weight-alternative-trilinear-decomposition- (SWATLD-) coupled high-performance liquid chromatography with DAD detection. The average recoveries attained from PARAFAC-ALS and SWATLD with the factor number of 4 (N=4) were101±5% and102±4% for vancomycin, and96±3% and97±3% for cephalexininde in real human samples, respectively. The statistical comparison between PARAFAC-ALS and SWATLD is demonstrated to be similar. The results indicated that the combination of HPLC-DAD detection with second-order calibration algorithms is a powerful tool to quantify the analytes of interest from overlapped chromatographic profiles for complex analysis of drugs in plasma.

scholarly journals Determination of Psoralen in Human Plasma Using Excitation-Emission Matrix Fluorescence Coupled to Second-order Calibration

2008 ◽  
Vol 24 (9) ◽  
pp. 1171-1176 ◽  
Author(s):  
A-Lin XIA ◽  
Hai-Long WU ◽  
Shao-Hua ZHU ◽  
Qing-Juan HAN ◽  
Yan ZHANG ◽  
...  
Keyword(s):  
Human Plasma ◽  
Second Order ◽  
Excitation Emission Matrix ◽  
Second Order Calibration
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