High-resolution three-dimensional imaging of the lateral plasma membrane of cochlear outer hair cells by atomic force microscopy

2002 ◽  
Vol 451 (1) ◽  
pp. 62-69 ◽  
Author(s):  
Christian Le Grimellec ◽  
Marie-C�cile Giocondi ◽  
Marc Lenoir ◽  
Marianne Vater ◽  
G�rard Sposito ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Hair Cells ◽  
Three Dimensional ◽  
Outer Hair Cells ◽  
Three Dimensional Imaging ◽  
Force Microscopy ◽  
Atomic Force

Influence of Different Liquid Environments on Atomic Force Microscopy Detection of Living bEnd.3 Cells

2021 ◽  
Author(s):  
Jin Yan ◽  
Baishun Sun ◽  
Chenchen Xie ◽  
Yan Liu ◽  
Zhengxun Song ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Atomic Force Microscope ◽  
Three Dimensional ◽  
Biomedical Science ◽  
Three Dimensional Imaging ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscope (AFM) is one of the most important tools in the field of biomedical science, which can be used to perform the high-resolution three-dimensional imaging of samples in...

scholarly journals Acoustic subsurface-atomic force microscopy: Three-dimensional imaging at the nanoscale

2021 ◽  
Vol 129 (3) ◽  
pp. 030901
Author(s):  
Hossein J. Sharahi ◽  
Mohsen Janmaleki ◽  
Laurene Tetard ◽  
Seonghwan Kim ◽  
Hamed Sadeghian ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Force Microscopy ◽  
Atomic Force

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

Three-dimensional imaging of undercut and sidewall structures by atomic force microscopy

2011 ◽  
Vol 82 (2) ◽  
pp. 023707 ◽  
Author(s):  
Sang-Joon Cho ◽  
Byung-Woon Ahn ◽  
Joonhui Kim ◽  
Jung-Min Lee ◽  
Yueming Hua ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Force Microscopy ◽  
Atomic Force

Imaging of the cortical cytoskeleton of guinea pig outer hair cells using atomic force microscopy

2004 ◽  
Vol 187 (1-2) ◽  
pp. 51-62 ◽  
Author(s):  
Hiroshi Wada ◽  
Kei Kimura ◽  
Takashi Gomi ◽  
Michiko Sugawara ◽  
Yukio Katori ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Guinea Pig ◽  
Hair Cells ◽  
Outer Hair Cells ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cortical Cytoskeleton

STUDIES ON MAGNETIC NANOMATERIALS BY ATOMIC FORCE MICROSCOPY WITH HIGH RESOLUTION

2005 ◽  
Vol 19 (09n10) ◽  
pp. 469-472 ◽  
Author(s):  
X. H. YANG ◽  
Y. F. WANG ◽  
A. P. LIU ◽  
H. Z. XIN ◽  
J. C. LIU
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Magnetic Materials ◽  
Scanning Tunneling Microscope ◽  
Three Dimensional ◽  
Magnetic Nanomaterials ◽  
Scanning Tunneling ◽  
Tunneling Microscope ◽  
Force Microscopy ◽  
Atomic Force

Studies on magnetic nanomaterials by atomic force microscopy (AFM) with high resolution are introduced in this paper. We have developed AFM.IPC-208B to observe the microsurface of magnetic fluid and doped cadmium sulfide (CdS·X) , which are two new types of magnetic nanomaterials. By using scanning tunneling microscope to detect the fluctuation of cantilever, we have obtained AFM three-dimensional images of samples, and analyzed the microstructures of the magnetic materials and their magnetism characteristics.

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